1887

Abstract

Summary

We have investigated the molecular basis for differences that we observed in the biological activities of genetically related but distinguishable human papillomavirus type 6 (HPV-6) subtypes. To analyse tissue-specific differences in replication and transcription, and to identify viral gene products important in the benign transformation of epithelial cells, we have modified procedures utilizing guanidinium isothiocyanate and density gradient centrifugation to facilitate the extraction of relatively undegraded DNA and RNA from 21 biopsy specimens of respiratory tract papillomata. Southern transfer analysis was used to characterize the viral genome, and to demonstrate that relative quantities of viral DNA in lesions varied but that the range was similar in lesions induced by HPV-6c, -6d, -6e and -6f. Dot blot analysis of the amount of viral RNA in comparison with the amount of 28S ribosomal RNA indicated that the relative level of viral RNA in each lesion varied considerably and that on average there was approximately twice as much viral RNA in HPV-6c-induced lesions as in HPV-6d-, -6e- or -6f-induced lesions. In dot blot and Northern analyses, hybridization of RNA from HPV-6c-induced lesions with HPV-6c DNA gave a stronger signal than hybridization of the same RNA with an HPV-6e probe, and vice versa. These differences in hybridization intensities with subtype-specific probes indicate that the most abundant RNA species are transcribed from parts of the genome that show sequence divergence between these two subtypes. Northern analysis demonstrated the predominant viral transcript to be about 1200 nucleotides in length in lesions induced by each of the four subtypes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-7-1671
1988-07-01
2022-01-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/7/JV0690071671.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-7-1671&mimeType=html&fmt=ahah

References

  1. AMTMANN E., SAUER G. 1982; Bovine papilloma virus transcription: polyadenylated RNA species and assessment of the direction of transcription. Journal of Virology 43:59–66
    [Google Scholar]
  2. BOIRON M., LEVY J. P., THOMAS M., FRIEDMAN J. C., BERNARD J. 1964; Some properties of bovine papillomavirus. Nature, London 201:423–424
    [Google Scholar]
  3. BROKER T. R., CHOW L. T. 1986 Human papillomaviruses of the genital mucosa: electron microscopic analyses of DNA heteroduplexes formed with HPV types 6,11, and 18. Cancer Cells 4 DNA Tumor Viruses 589–594 Edited by Botchan M., Grodzicker T., Sharp P. A. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  4. CHEN E. Y., HOWLEY P. M., LEVENSON A. D., SEEBERG P. H. 1982; The primary structure and genetic organization of the bovine papillomavirus type 1 genome. Nature, London 299:529–534
    [Google Scholar]
  5. CHIRGWIN J. M., PRZYBYLA A. E., MACDONALD R. J., ROTTER W. J. 1979; Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry 18:5294–5299
    [Google Scholar]
  6. CHOW L. T., PELLETIER A. G., GALLI R., BRINCKMANN U., CHIN M., ARVAN D., CAMPANELLI D., CHENG S., BROKER T. R. 1986 Transcription of human papillomavirus types 1 and 6. Cancer Cells 4 DNA Tumor Viruses 603–614 Edited by Botchan M., Grodzicker T., Sharp P. A. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  7. CHOW L. T., NASSERI M., WOLINSKY S. M., BROKER T. R. 1987; Human papillomavirus types 6 and 11 mRNAs from genital condylomata acuminata. Journal of Virology 61:2581–2588
    [Google Scholar]
  8. DARTMANN K., SCHWARZ E., GISSMANN L., ZUR HAUSEN H. 1986; The nucleotide sequence and genome organization of human papillomavirus type 11. Virology 151:124–130
    [Google Scholar]
  9. DENHARDT D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  10. DVORETZKY L, SHOBER R., CHATTOPADHYAY S. K., LOWY D. R. 1980; A quantitative in vitro focus assay in mouse cells for bovine papillomavirus. Virology 103:369–375
    [Google Scholar]
  11. ERICKSON J. M., RASHFORD C L., DORNEY D. J., WILSON G. N., SCHMICKEL R. D. 1981; Structure and variation of human ribosomal DNA: molecular analysis of cloned fragments. Gene 16:1–9
    [Google Scholar]
  12. FREESE U. K., SCHULTE P., PFISTER H. 1982; Papilloma virus-induced tumors contain a virus-specific transcript. Virology 117:257–261
    [Google Scholar]
  13. GISSMANN L., DUHL V., SCHULTZ-COULON H., ZUR HAUSEN H. 1982; Molecular cloning and characterization of human papillomavirus DNA from a laryngeal papilloma. Journal of Virology 44:393–400
    [Google Scholar]
  14. GLISIN V. R., CRKVENJAKOV R., BYUS C. 1974; Ribonucleic acid isolated by cesium chloride centrifugation. Biochemistry 13:2633–2637
    [Google Scholar]
  15. GOLDBERG D. A. 1980; Isolation and partial characterization of the drosophila alcohol dehydrogenase gene. Proceedings of the National Academy of Sciences, U.S.A 77:5794–5798
    [Google Scholar]
  16. HEILMAN C. A., ENGEL L., LOWY D. R., HOWLEY P. M. 1982; Virus specific transcription in bovine papillomavirus-transformed mouse cells. Virology 119:22–34
    [Google Scholar]
  17. LEHN H., ERNST T.-M., SAUER G. 1984; Transcription of episomal papillomavirus DNA in human condylomata acuminata and Buschke-Löwenstein tumours. Journal of General Virology 65:2003–2010
    [Google Scholar]
  18. LUSKY M., BOTCHAN M. R. 1985; Genetic analysis of the bovine papillomavirus type 1 trans-acting replication factors. Journal of Virology 53:955–965
    [Google Scholar]
  19. MOUNTS P., KASHIMA H. 1984; Association of human papillomavirus subtype and clinical course in respiratory papillomatosis. Laryngoscope 94:28–33
    [Google Scholar]
  20. MOUNTS P., KELLY T. I. 1984; Rearrangements of host and viral DNA in mouse cells transformed by simian virus 40. Journal of Molecular Biology 177:431–460
    [Google Scholar]
  21. MOUNTS P., SHAH K. V., KASHIMA H. 1982; Viral etiology of juvenile and adult onset squamous papilloma of the larynx. Proceedings of the National Academy of Sciences, U.S.A 79:5425–5429
    [Google Scholar]
  22. MOUNTS P., WU T.-C., LEVENTHAL B. G., KASHIMA H., DEDO H., SINGLETON G., GALL S., WECK P., WHISNANT J. 1985 Analysis of human papillomavirus type 6 in the respiratory and genital tracts during interferon therapy. UCLA Symposia on Molecular and Cellular Biology, New Series 32 Papillomaviruses: Molecular and Clinical Aspects 137–154 Edited by Howley P. M., Broker T. R. New York: Alan R. Liss;
    [Google Scholar]
  23. NASSERI M., HIROCHIKA R., BROKER T. R., CHOW L. T. 1987; A human papilloma virus type 11 transcript encoding an E1 E4 protein. Virology 159:433–439
    [Google Scholar]
  24. OLSEN C, GORDON D. E., ROBL M. G., LEE K. P. 1969; Oncogenicity of bovine papilloma virus. Archives of Environmental Health 19:827–837
    [Google Scholar]
  25. PEDEN K., PIPAS J. M., PEARSON-WHITE S., NATHANS D. 1980; Isolation of mutants of an animal virus in bacteria. Science 290:1392–1396
    [Google Scholar]
  26. PEDEN K., MOUNTS P., HAYWARD G. S. 1982; Homology between mammalian DNA sequences and human herpesvirus genomes detected by a hybridization procedure with high complexity probe. Cell 31:71–80
    [Google Scholar]
  27. SARVER N., RABSON M. S., YANG Y.-C., HOWLEY P. M. 1984; Localization and analysis of bovine papillomavirus type 1 transforming functions. Journal of Virology 52:377–388
    [Google Scholar]
  28. SCHWARZ E., DÜRST M., DEMANKOWSKI C, LATTERMAN O., ZECH R., WOLFSPERGER E., SUHAI S., ZUR HAUSEN H. 1983; DNA sequence and genome organization of genital human papillomavirus type 6b. EMBO Journal 2:2341–2348
    [Google Scholar]
  29. SOUTHERN E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  30. SPALHOLZ B. A., YANG Y.-C., HOWLEY P. M. 1985; Transactivation of a bovine papillomavirus transcriptional regulatory element by the E2 gene product. Cell 42:183–191
    [Google Scholar]
  31. STEINBERG B. M., TOPP W., SCHNEIDER P. S., ABRAMSON A. 1983; Laryngeal papillomavirus infection during clinical remission. New England Journal of Medicine 308:1261–1265
    [Google Scholar]
  32. SUISSA M. 1983; Spectrophotometric quantitation of silver grains eluted from autoradiograms. Analytical Biochemistry 133:511–514
    [Google Scholar]
  33. THOMAS P. S. 1980; Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose. Proceedings of the National Academy of Sciences, U.S.A 77:5201–5205
    [Google Scholar]
  34. WARD P., WU T.-C., MOUNTS P. 1987 RNA probes to analyze human papillomavirus gene expression in squamous papilloma of the respiratory tract. Cancer Cells 5 Papillomaviruses 73–78 Edited by Steinberg B. M., Brandsma J. L., Taichman L. B. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. WHITE B. A., BANCROFT F. C. 1982; Cytoplasmic dot hybridization. Journal of Biological Chemistry 257:8569–8572
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-7-1671
Loading
/content/journal/jgv/10.1099/0022-1317-69-7-1671
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error