1887

Abstract

Summary

Non-cytopathic strains of bovine viral diarrhoea virus (BVDV) readily establish persistent infections in cells of bovine origin. The involvement of endogenous interferon (IFN) on the maintenance of the infection level, as well as the effect of exogenous IFN and tumour necrosis factor alpha (TNF-α), was studied. Although exogenous IFN suppressed the spread and replication of virus, it did not cure the infection, even when continuously present over many cell passages. TNF-α alone had no antiviral effect in this system. However, both TNF-α and IFN enhanced the cytopathic effect of cytopathic BVDV, and induced a BVDV-like cytopathic effect in cells infected with non-cytopathic BVDV. These data are discussed with regard to possible mechanisms of pathogenesis during fatal BVDV disease in cattle.

Keyword(s): BVDV , interferon (BoIFN) and TNF-α
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/content/journal/jgv/10.1099/0022-1317-69-6-1399
1988-06-01
2022-01-27
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References

  1. BIELEFELDT OHMANN H. 1988a; BVD virus antigens in tissues of persistently viraemic, clinically normal cattle: implications for the pathogenesis of clinically fatal disease. Acta veterinaria scandinavica in press
    [Google Scholar]
  2. BIELEFELDT OHMANN H. 1988b; In situ characterization of mononuclear leukocytes in skin and digestive tract persistently BVD virus-infected, clinically healthy calves and calves with mucosal disease. Veterinary Pathology in press
    [Google Scholar]
  3. BIELEFELDT OHMANN H., BLOCH B. 1982; Electron microscopic studies of bovine viral diarrhoea virus in tissues of diseased calves and in cell cultures. Archives of Virology 71:57–74
    [Google Scholar]
  4. BIELEFELDT OHMANN H., RONSHOLT L., BLOCH B. 1987; Demonstration of bovine viral diarrhoea virus in peripheral blood mononuclear cells of persistently infected, clinically normal cattle. Journal of General Virology 68:1971–1982
    [Google Scholar]
  5. BOLIN S. R., MCCLURKIN A. W., CUTLIP R. C., CORIA M. F. 1985; Response of cattle persistently infected with noncytopathic bovine viral diarrhea virus to vaccination for bovine viral diarrhea and to subsequent challenge exposure with cytopathic bovine viral diarrhea virus. American Journal of Veterinary Research 46:2467–2470
    [Google Scholar]
  6. BROWNLIE J., CLARKE M. C, HOWARD C. I., POCOCK D. H. 1987; Pathogenesis and epidemiology of bovine viral diarrhoea virus infection in cattle. Annales de Recherches Vétérinaires 18:157–166
    [Google Scholar]
  7. CAMPOS M., BIELEFELDT OHMANN H., HUGHES G., BABIUK L. A., LAWMAN M. J. P. 1987; Studies on the in vitro biological activities of recombinant bovine tumor necrosis factor (ᴦBoIFN) alpha. I. Synergistic antiviral efficacy of ᴦBoIFN alpha and recombinant bovine interferon gamma (ᴦBoIFN gamma). lmmunobiology 175:92
    [Google Scholar]
  8. CZARNIECKI C. W., HAMILTON E. B., FENNIE C. W., WOLF R. L. 1986; In vitro biological activities of E. coli-derived bovine interferon-α, -β and -γ. Journal of Interferon Research 6:20–37
    [Google Scholar]
  9. DONE J. F., TERLECKI S., RICHARDSON -C, HARKNESS I. W., SANDS I. J., PATTERSON D. S. P., SWEASEY D., SHAW I. G., WINKLER C. E., DUFFELL S. J. 1980; Bovine virus diarrhoea-mucosal disease virus: pathogenicity for the fetal calf following maternal infection. Veterinary Record 106:473–479
    [Google Scholar]
  10. DONIS R. O., DUBOVI E. J. 1987; Differences in virus-induced polypeptides in cells infected by cytopathic and noncytopathic biotypes of bovine virus diarrhea-mucosal disease virus. Virology 158:168–173
    [Google Scholar]
  11. IKEDA T., LEVER M. L., THOMAS H. C. 1986; Evidence for a deficiency of interferon production in patients with chronic hepatitis B virus infection acquired in adult life. Hepatology 6:962–965
    [Google Scholar]
  12. JACOBSON S., MCFARLAND H. F. 1982; Measles virus persistence in human lymphocytes: a role for virus-induced interferon. Journal of General Virology 63:351–357
    [Google Scholar]
  13. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. MIMS C. A. 1986; Interactions of viruses with the immune system. Clinical and Experimental Immunology 66:1–16
    [Google Scholar]
  15. PREBLE O. T., FRIEDMAN R. M. 1983; Biology of disease. Interferon-induced alterations in cells: relevance to viral and nonviral diseases. Laboratory Investigation 49:4–18
    [Google Scholar]
  16. TOWBIN H., STAEHELIN F., GORDON J. 1979; Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
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