1887

Abstract

Summary

Twelve temperature-sensitive mutants were isolated from nuclear polyhedrosis virus grown in cells and were sorted into four groups by their properties in plaque assays at the non-permissive temperature (32 °C). The phenotypes of the four groups were as follows: (i) failure to make polyhedra, (ii) few polyhedra formed, (iii) reduced plaquing efficiency, (iv) small plaque size. Ten mutants had reduced plaque size and polyhedra formation at 32 °C. One mutant formed plaques without polyhedra, had a reduced infectious virus titre at 32 °C and also showed a defect in late gene function. Two mutants formed small plaques with few polyhedra and were temperature-sensitive with respect to production of extracellular non-occluded virions at 32 °C. Other phenotypes were also distinguished. The formation of polyhedra by all the mutants was 2 to 4 h faster at 32 °C than at 25 °C. After temperature shift-up from 25 °C to 32 °C at 12 h post-infection polyhedron formation was still 2 to 4 h faster. Complementation analyses based on polyhedron formation in double infections at 32 °C distinguished four complementation groups.

Keyword(s): baculovirus , NPV and ts mutants
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/content/journal/jgv/10.1099/0022-1317-69-6-1299
1988-06-01
2024-12-06
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References

  1. BROWN M., FAULKNER P. 1980; A partial genetic map of the baculovirus, Autographa californica nuclear polyhedrosis virus, based on recombination studies with ts mutants. Journal of General Virology 48:247–251
    [Google Scholar]
  2. BROWN M., CRAWFORD A. M., FAULKNER P. 1979; Genetic analysis of a baculovirus Autographa californica nuclear polyhedrosis virus. 1. Isolation of temperature-sensitive mutants and assortment into complementation groups. Journal of Virology 31:190–198
    [Google Scholar]
  3. CAIRNS I. 1960; The initiation of vaccinia infection. Virology 11:603–623
    [Google Scholar]
  4. CHOE Y. K., BYUN S. M., LEE H. H., CHUNG T. W. 1986; A nuclear polyhedrosis virus of Hyphantria cunea: replication in vitro. Biotechnology Letters 8:853–858
    [Google Scholar]
  5. DUNCAN R., FAULKNER P. 1982; Bromodeoxyuridine-induced mutants of Autographa californica nuclear polyhedrosis virus defective in occlusion body formation. Journal of General Virology 62:369–373
    [Google Scholar]
  6. GORDON J. D., CARSTENS E. B. 1984; Phenotypic characterization and physical mapping of a temperature-sensitive mutant of Autographa californica nuclear polyhedrosis virus defective in DNA synthesis. Virology 138:69–81
    [Google Scholar]
  7. HARRAP K. A. 1972; The structure of nuclear polyhedrosis viruses. III. Virus assembly. Virology 50:133–139
    [Google Scholar]
  8. HINK W. F., STRAUSS E. 1976; Replication and passage of alfalfa looper NPV plaque variants in cloned cell cultures and larval stages of four host species. Journal of Invertebrate Pathology 27:49–55
    [Google Scholar]
  9. KNUDSON D. L., TINSLEY T. W. 1974; Replication of a nuclear polyhedrosis virus in a continuous cell culture of Spodoptera frugiperda: purification, assay of infectivity, and growth characteristics of the virus. Journal of Virology 14:934–944
    [Google Scholar]
  10. KNUDSON D. L., HARRAP K. A. 1976; Replication of a nuclear polyhedrosis virus in a continuous cell culture of Spodoptera frugiperda: microscopy study of the sequence of events of the virus infection. Journal of Virology 17:254–268
    [Google Scholar]
  11. LEE H. H. 1987 Replication and cloning of Hyphantria cunea nuclear polyhedrosis virus in Spodoptera frugiperda cell line. Hanguk Journal of Genetic Engineering 21–6 Seoul: Kon Kuk University;
    [Google Scholar]
  12. LEE H. H., MILLER L. K. 1978; Isolation of genotypic variants of Autographa californica nuclear polyhedrosis virus. Journal of Virology 27:754–767
    [Google Scholar]
  13. LEE H. H., MILLER L. K. 1979; Isolation, complementation, and initial characterization of temperature-sensitive mutants of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 31:240–252
    [Google Scholar]
  14. MILLER L. K. 1981; Construction of a genetic map of the baculovirus Autographa californica nuclear polyhedrosis virus by marker rescue of temperature-sensitive mutants. Journal of Virology 39:973–980
    [Google Scholar]
  15. MILLER L. K., DAWES K. P. 1979; Physical map of the DNA genome of Autographa californica nuclear polyhedrosis virus. Journal of Virology 29:1044–1055
    [Google Scholar]
  16. MIN E. S., LEE H. H. 1982; Electron microscopic visualization of temperature-sensitivity of Autographa californica NPV ts-B1074 mutant. Journal of the Korean Society of Virology 12:49–54
    [Google Scholar]
  17. OH C. K., LEE H. H. 1987; Electron microscope studies of infection and multiplication of Autographa californica NPV clone L-1 and ts-B1074. 1. Infection and multiplication of A. californica NPV L-1. Journal of the Korean Society of Virology 17:61–72
    [Google Scholar]
  18. POTTER K. N., FAULKNER P., MACKINNON E. A. 1976; Strain selection during serial undiluted passage of Trichoplusia ni nuclear polyhedrosis virus. Journal of Virology 18:1040–1050
    [Google Scholar]
  19. ROHEL D. Z., COCHRAN M. A., FAULKNER P. 1983; Characterization of two abundant mRNAs of Autographa californica nuclear polyhedrosis virus present late in infection. Virology 124:357–365
    [Google Scholar]
  20. SMITH G. E., SUMMERS M. D. 1979; Restriction maps of five Autographa californica MNPV variants, Trichoplusia ni MNPV, and Galleria mellonella MNPV DNAs with endonucleases SmaI, KpnI, BamHI, Sacl, XhoI, and EcoRI. Journal of Virology 30:828–838
    [Google Scholar]
  21. SUMMERS M. D., ARNOTT H. J. 1969; Ultrastructural studies on inclusion formation and virus occlusion in nuclear polyhedrosis and granulosis virus-infected cells of Trichoplusia ni (Hübner). Journal of Ultrastructure Research 28:462–80
    [Google Scholar]
  22. SUMMERS M. D., SMITH G. E. 1978; Baculovirus structural polypeptides. Virology 84:390–402
    [Google Scholar]
  23. VAUGHN J. L., GOODWIN R. H., TOMKINS G. J., MCCAWLEY P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13:213–217
    [Google Scholar]
  24. VLAK I. M. 1981; Mapping of BamHI and SmaI DNA restriction sites on the genome of the nuclear polyhedrosis virus of the alfalfa looper, Autographa californica. Journal of Invertebrate Pathology 36:409–414
    [Google Scholar]
  25. VLAK J. M., SMITH G. E., SUMMERS M. D. 1981; Hybridization selection and in vitro translation of Autographa californica nuclear polyhedrosis virus mRNA. Journal of Virology 40:762–771
    [Google Scholar]
  26. VOLKMAN L. E., SUMMERS M. D. 1977; Autographa californica NPV: comparative infectivity of the occluded, alkali-liberated, and nonoccluded forms. Journal of Invertebrate Pathology 30:102–103
    [Google Scholar]
  27. VOLKMAN L. E., SUMMERS M. D., HSIEH C. H. 1976; Occluded and nonoccluded nuclear polyhedrosis virus grown in Trichoplusia ni: comparative neutralization, comparative infectivity, and in vitro growth studies. Journal of Virology 19:820–832
    [Google Scholar]
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