1887

Abstract

Summary

Viral genomic RNA was purified from BHK-21 cells infected with epizootic haemorrhagic disease virus and the 10 dsRNA genome segments were isolated by polyacrylamide gel electrophoresis. These genome segments were translated using the rabbit reticulocyte lysate system and the synthesized proteins were detected by immune precipitation and gel electrophoresis. This allowed the assignment of protein coding to the genome segments and the identification of two additional virus-specified proteins not readily detectable in lysates of virus-infected cells.

Keyword(s): dsRNA , EHDV and translation
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/content/journal/jgv/10.1099/0022-1317-69-6-1255
1988-06-01
2019-10-22
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-6-1255
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