1887

Abstract

Summary

plants were examined by electron microscopy 5 months after inoculation with Sonchus yellow net virus (SYNV). No virions were observed in leaf or root cells, but cells in sections of calyx tissue contained large numbers of virus particles. Most of these particles were only 73 to 86% as long as particles of standard SYNV. inoculated with sap extracted from chronically infected calyx became systemically infected but exhibited chlorotic mottling, instead of the normal vein-clearing symptoms. Most virus particles in these plants were short, and when purified, sedimented more slowly than standard SYNV. Purified short particles were not infective, but plants inoculated with a mixture of short and standard particles developed mottling symptoms and yielded predominantly short particles. Proteins from short particles were electrophoretically and antigenically identical to those from standard virus. RNA from short particles was 77% the size of RNA from standard SYNV and hybridized to cloned SYNV cDNA. These short particles have all the characteristics of defective interfering particles.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-5-999
1988-05-01
2022-08-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/5/JV0690050999.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-5-999&mimeType=html&fmt=ahah

References

  1. ADAM G., GAEDIGK K., MUNDRY K. W. 1983; Alterations of a plant rhabdovirus during successive mechanical transfers. Zeitschrift für Pflanzenkrankheit und Pflanzenschutz 90:28–35
    [Google Scholar]
  2. BLACK L. M. 1979; Vector-cell monolayers and plant viruses. Advances in Virus Research 25:196–266
    [Google Scholar]
  3. BOWEN B., STEINBERG J., LAEMMLI J. K., WEINTRAUB H. 1980; The detection of DNA-binding proteins by protein-blotting. Nucleic Acids Research 8:1–20
    [Google Scholar]
  4. CHRISTIE S. R., CHRISTIE R. G., EDWARDSON J. R. 1974; Transmission of a bacilliform virus of sowthistle and Bidens pilosa. Phytopathology 64:840–845
    [Google Scholar]
  5. CROWLEY N. C, HARRISON B. D., FRANCKI R. I. B. 1965; Partial purification of lettuce necrotic yellows virus. Virology 26:290–296
    [Google Scholar]
  6. FALK B., PURCIFULL D. E., CHRISTIE S. R. 1986; Natural occurrence of Sonchus yellow net virus in Florida lettuce. Plant Disease 70:591–593
    [Google Scholar]
  7. FAULKNER G. P., LAZZARINI R. A. 1980 Homologous interference by defective virus particles. Rhabdoviruses 2163–176 Edited by Bishop D. H. L. Boca Raton: CRC Press;
    [Google Scholar]
  8. FRANCKI R. I. B., RANDLES J. W. 1980 Rhabdoviruses infecting plants. Rhabdoviruses 3135–165 Edited by Bishop D. H. L. Boca Raton: CRC Press;
    [Google Scholar]
  9. GUSTAFSON G. D., MILNER J. J., MCFARLAND J. E., PEDERSON K., LARKINS B. A., JACKSON A. O. 1982; Investigation of the complexity of barley stripe mosaic virus RNAs with recombinant DNA clones. Virology 120:182–193
    [Google Scholar]
  10. HAWKES R., NIDGY E., GORDON J. 1982; A dot-immunobinding assay for monoclonal and other antibodies. Analytical Biochemistry 119:142–147
    [Google Scholar]
  11. HARRISON B. D. 1970; Tobacco rattle virus. CMI/AAB Descriptions of Plant Viruses12
    [Google Scholar]
  12. HEATON L. A., HILLMAN B. I., ZUIDEMA D., JACKSON A. O. 1987; Sonchus yellow net virus: a plant rhabdovirus with six genes. Seventh International Congress for Virology Abstract No. R33.7 225
    [Google Scholar]
  13. HOLLAND J. J., KENNEDY I. T., SEMLER B. L., JONES C L., ROUX L., GRABAU E. A. 1980 Defective interfering RNA viruses and the host cell response. Comprehensive Virology 16137–192 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  14. HUANG A. S. 1973; Defective-interfering viruses. Annual Review of Microbiology 27:101–117
    [Google Scholar]
  15. HUANG A. S., BALTIMORE D. 1977 Defective-interfering animal viruses. Comprehensive Virology 1073–116 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  16. ISMAIL I. D., HAMILTON I. D., ROBERTSON E., MILNER J. J. 1987; Movement and intracellular location of Sonchus yellow net virus within infected Nicotiana edwardsonii. Journal of General Virology 68:2429–2438
    [Google Scholar]
  17. JACKSON A. O., CHRISTIE S. R. 1977; Purification and some physicochemical properties of Sonchus yellow net virus. Virology 77:344–355
    [Google Scholar]
  18. JOHNSON L. D., LAZZARINI R. A. 1980 RNA synthesis by defective interfering vesicular stomatitis virus particles. Rhabdoviruses 2117–139 Edited by Bishop D. H. L. Boca Raton: CRC Press;
    [Google Scholar]
  19. LAEMMLI U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  20. LIN M. J., CAMPBELL R. N. 1972; Characterization of broccoli necrotic yellows virus. Virology 48:30–40
    [Google Scholar]
  21. PETERS D. 1981; Plant rhabdovirus group. CMI/AAB Descriptions of Plant Viruses244
    [Google Scholar]
  22. REZAIAN M. A., HEATON L. A., PEDERSON K., MILNER J. J., JACKSON A. O. 1983; Size and complementarity of polyadenylated RNAs induced in tobacco infected with Sonchus yellow net virus. Virology 144:342–351
    [Google Scholar]
  23. SHIRAKO Y., EHARA Y. 1986; Comparison of the in vitro translation products of wild-type and a deletion mutant of soil-borne wheat mosaic virus. Journal of General Virology 67:1237–1245
    [Google Scholar]
  24. TOWBIN H., STAEHELIN T., GORDON J. 1979; Electrophoretic transfer of proteins from Polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, U.S.A 76:4350–4354
    [Google Scholar]
  25. VERKLEU F. N., PETERS D. 1983; Characterization of a defective form of tomato spotted wilt virus. Journal of General Virology 64:677–686
    [Google Scholar]
  26. YOUNGNER J. S., PREBLE O. T. 1980 Viral persistence: evolution of viral populations. Comprehensive Virology 1673–136 Edited by Fraenkel-Conrat H., Wagner R. R. New York: Plenum Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-5-999
Loading
/content/journal/jgv/10.1099/0022-1317-69-5-999
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error