1887

Abstract

Summary

Human sera were examined by immunoblotting for antibodies against the polymerase (reverse transcriptase) believed to be encoded in the P open reading frame (ORF) of human hepatitis B virus (HBV). Sera from patients with self-limited and chronic hepatitis reacted specifically with fusion proteins containing different domains of the P ORF. The results indicate that this ORF is expressed, and that the corresponding proteins contain at least two immunogenic domains. In contrast to human immunodeficiency virus, induction of antibodies against reverse transcriptase appears to be less common for HBV, and may depend on long persistence of infection.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-69-3-689
1988-03-01
2022-01-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/69/3/JV0690030689.html?itemId=/content/journal/jgv/10.1099/0022-1317-69-3-689&mimeType=html&fmt=ahah

References

  1. DL MARZO VERONESE F., COPELAND T. D., DEVICO A. L., RAHMAN R., OROSZLAN S., GALLO R. C., SARNGADHARAN M. G. 1986; Characterization of highly immunogenic p66/p51 as the reverse transcriptase of HTLV-III/LAV. Science 231:1289–1291
    [Google Scholar]
  2. ELFASSI E., HASELTINE W. A., DIENSTAG J. L. 1986; Detection of hepatitis B virus X product using an open reading frame Escherichia coli expression vector. Proceedings of the National Academy of Sciences, U.S.A 83:2219–2222
    [Google Scholar]
  3. GERSHONI J. M., PALADE G. E. 1983; Protein blotting: principles and applications. Analytical Biochemistry 131:1–15
    [Google Scholar]
  4. KAY A., MANDART E., TREPO C., GALIBERT F. 1985; The HBV HBx gene expressed in E. coli is recognised by sera from hepatitis patients. EMBO Journal 4:1287–1292
    [Google Scholar]
  5. KüPPER H., DELAMATER J., OTTO B., SCHALLER H. 1982 Expression of major foot and mouth disease antigen in E. coli. Proceedings of the Fourth International Symposium on Genetics of Industrial Microorganisms222–226 Edited by Ikeda Y., Beppu T. Kyoto: Kodansha;
    [Google Scholar]
  6. MEYERS M. L., VIVITSKI-TREPO L., NATH N., SNINSKY J. J. 1986; Hepatitis B polypeptides X: expression in Escherichia coli and identification of specific antibodies in sera from hepatitis B virus-infected humans. Journal of Virology 57:101–109
    [Google Scholar]
  7. MORIARTY A. M., ALEXANDER H., LERNER R. A., THORNTON G. B. 1985; Antibodies to peptides detect new hepatitis B antigen: serological correlation with hepatocellular carcinoma. Science 227:429–433
    [Google Scholar]
  8. PATARCA R., HASELTINE W. A. 1984; Sequence similarities among retroviruses. Nature, London 309:728
    [Google Scholar]
  9. REMAUT E., STANSSENS P., FIERS W. 1981; Plasmid vectors for high efficiency expression controlled by the PL promotor of coliphage lambda. Gene 15:81–93
    [Google Scholar]
  10. STANLEY K. K., LUZIO J. P. 1984; Construction of a new family of high efficiency bacterial expression vectors: identification of cDNA clones coding for human liver proteins. EMBO Journal 3:1429–1434
    [Google Scholar]
  11. STREBEL K., BECK E., STROHMAIER K., SCHALLER H. 1986; Characterization of foot-and-mouth disease virus gene products with antisera against bacterially synthesized fusion proteins. Journal of Virology 57:983–991
    [Google Scholar]
  12. SUMMERS J., MASON W. 1982; Replication of the genome of a hepatitis B-1ike virus by reverse transcription of an RNA intermediate. Cell 29:403–415
    [Google Scholar]
  13. TIOLLAIS P., POURCEL C., DEJEAN A. 1985; The hepatitis B virus. Nature, London 317:489–495
    [Google Scholar]
  14. TOH H., HAYASHIDA H., MIYATA T. 1983; Sequence homology between retroviral reverse transcriptase and putative polymerase of hepatitis B virus and cauliflower mosaic virus. Nature, London 305:827–829
    [Google Scholar]
  15. WILL H., SALFELD J., PFAFF E., MANSO C, THEILMANN L., SCHALLER H. 1986; Putative reverse transcriptase intermediates of human hepatitis B virus in primary liver carcinomas. Science 231:594–596
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-69-3-689
Loading
/content/journal/jgv/10.1099/0022-1317-69-3-689
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error