1887

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Volume 69, Issue 2

Red Clover Mottle Comovirus B-RNA Spreads between Cells in Tobamovirus-infected Tissues Free

Abstract

Summary

B component RNA (B-RNA) of red clover mottle comovirus (RCMV) was not transported between cells of inoculated leaf tissue unless it was co-inoculated with the M component. However when host plants were infected with sunn-hemp mosaic tobamovirus (SHMV) or mutant Ni118 of tobacco mosaic virus (TMV) before superinoculation, RCMV B-RNA was transported readily between cells. Plants were infected with SHMV or Ni118 and, 5 days after superinoculation with RCMV B component, protoplasts were isolated from the infected leaves and inoculated with RCMV M component. About 30% of such protoplasts multiplied RCMV (identified by immunofluorescence microscopy) whereas only 3 to 5% of protoplasts from similarly treated plants not initially infected with SHMV or Ni118 multiplied RCMV. Thus B-RNA spread from mixedly infected (SHMV + B-RNA or Ni118 + B-RNA) cells in the absence of M-RNA to the neighbouring cells. RCMV B-RNA spread in leaves infected with the temperature-sensitive coat protein TMV mutant Ni118 and grown at non-permissive temperature. Thus TMV coat protein is not involved in enabling RCMV RNA to be transported.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): RCMV , TMV and virus transport
© Society for General Microbiology 1988
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1988-02-01
2024-04-26
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References

  1. ATABEKOV J. G., DOROKHOV YU. L. 1984; Plant virus specific transport function and resistance of plants to viruses. Advances in Virus Research 29:313–364
     [Google Scholar]
  2. ATABEKOV J. C, NOVIKOV V. K., VISHNICHENKO V. K., KAFTANOVA A. S. 1970; Some properties of hybrid viruses reassembled in vitro. Virology 41:519–532
     [Google Scholar]
  3. ATABEKOV J. G., TALIANSKY M. E., DRAMPYAN A. H., KAPLAN I. B., TURKA I. E. 1984; Systemic infection by a phloem-restricted virus in parenchyma cells in a mixed infection. Biologicheskie Nauki 10:28–31 in Russian
    [Google Scholar]
  4. BARKER H. 1987; Invasion of non-phloem tissue in Nicotiana clevelandii by potato leafroll luteovirus is enhanced in plants also infected with potato Y potyvirus. Journal of General Virology 68:1223–1227
     [Google Scholar]
  5. BOSCH F. X., JOCKUSH H. 1972; Temperature-sensitive mutants of TMV: behaviour of a non-coat protein mutant in isolated tobacco cells. Molecular and General Genetics 116:95–98
     [Google Scholar]
  6. CARR R. J., KIM K. S. 1983; Evidence that bean golden mosaic virus invades non-phloem tissue in double infections with tobacco mosaic virus. Journal of General Virology 64:2489–2492
     [Google Scholar]
  7. FRANSSEN H., GOLDBACH R., BROEKHUUSEN M., MOERMAN M., VAN KAMMEN A. 1982; Expression of middle-component RNA of cowpea mosaic virus: in vitro generation of a precursor to both capsid proteins by a bottom-component RNA-encoded protease from infected cells. Journal of Virology 41:8–17
     [Google Scholar]
  8. GODEFROY-COLBURN T., GAGEY M.-J., BERNA A., STUSSI-GARAUD C. 1986; A non-structural protein of alfalfa mosaic virus in the walls of infected tobacco cells. Journal of General Virology 67:2233–2239
     [Google Scholar]
  9. GOLDBACH R., REZELMAN G., VAN KAMMEN A. 1980; Independent replication and expression of B-component RNA of cowpea mosaic virus. Nature, London 286:297–300
     [Google Scholar]
  10. HIBI T., REZELMAN G., VAN KAMMEN A. 1975; Infection of cowpea mesophyll protoplasts with cowpea mosaic virus. Virology 64:308–318
     [Google Scholar]
  11. HUBER R., REZELMAN G., HIBI T., VAN KAMMEN A. 1977; Cowpea mosaic virus infection of protoplasts from Samsun tobacco leaves. Journal of General Virology 34:315–323
     [Google Scholar]
  12. LAPCHIC L. G., KUZNETZOVA L. L., MELNICZENKO V. S., VOSELKO S. K., SJEDIN A. A. 1976a; Red clover mottle virus in Ukraine. Phytopathologische Zeitschrift 82:339–346
     [Google Scholar]
  13. LAPCHIC L. G., KUZNETZOVA L. L., MELNICZENKO V. S., KRASNOVA E. F. 1976b; Infectivity of the nucleoprotein components of red clover mottle virus. Phytopathologische Zeitschrift 85:1–6
     [Google Scholar]
  14. LEONARD D. H., ZAITLIN M. 1982; A temperature-sensitive strain of tobacco mosaic virus defective in cell-to-cell movement generates an altered viral-coded protein. Virology 117:416–420
     [Google Scholar]
  15. MALYSHENKO S. I., KAPLAN I. B., MUSHEGYAN A. R., TALIANSKY M. E., ATABEKOV J. G. 1985; Stimulation of potato virus X reproduction in the cells of susceptible and resistant plants. hvestia Akademii Nauk SSSR, Seria Biologicheskaya 3:339–343 in Russian
    [Google Scholar]
  16. MEYER M., HEMMER O., MAYO M. A., FRITSCH C. 1986; The nucleotide sequence of tomato black ring virus RNA-2. Journal of General Virology 67:1257–1271
     [Google Scholar]
  17. NISHIGUCHI M., MOTOYOSHI F., OSHIMA N. 1978; Behaviour of a temperature sensitive strain of tobacco mosaic virus in tomato leaves and protoplasts. Journal of General Virology 39:53–61
     [Google Scholar]
  18. OHNO T., TAKAMATSU N., MESHI T., OKADA Y., NISHLGUCHI M., KIHO Y. 1983; Single amino acid substitution in 30K protein of TMV defective in virus transport function. Virology 131:255–258
     [Google Scholar]
  19. REZELMAN G., FRANSSEN H. J., GOLDBACH R., IE T. S., VAN KAMMEN A. 1982; Limits to the independence of bottom component RNA of cowpea mosaic virus. Journal of General Virology 60:335–342
     [Google Scholar]
  20. SHANKS M., STANLEY J., LOMONOSSOFF G. P. 1986; The primary structure of red clover mottle virus middle component RNA. Virology 155:697–706
     [Google Scholar]
  21. TALIANSKY M. E., MALYSHENKO S. I., PSHENIKOVA E. S., KAPLAN I. B., ULANOVA E. F., ATABEKOV I. G. 1982a; Plant virus-specific transport function. I. Virus genetic control required for systemic spread. Virology 122:318–326
     [Google Scholar]
  22. TALIANSKY M. E., MALYSHENKO S. I., PSENIKOVA E. S., ATABEKOV J. G. 1982b; Plant virus-specific transport function. II. A factor controlling virus host range. Virology 122:327–332
     [Google Scholar]
  23. WATANABE J., OOSHIKA I., MESHI T., OKADA J. 1986; Subcellular localization of the 30K protein in TMV-inoculated tobacco protoplasts. Virology 152:414–420
     [Google Scholar]
  24. VAN WEZENBEEK P., VERVER J., HARMSEN J., VOS P., VAN KAMMEN A. 1983; Primary structure and gene organization of the middle component RNA of cowpea mosaic virus. EMBO Journal 2:941–946
     [Google Scholar]
  25. ZIMMERN D., HUNTER T. 1983; Point mutation in the 30K open reading frame of TMV implicated in temperature-sensitive assembly and local lesion spreading of mutant Ni2519. EMBO Journal1893–1900
     [Google Scholar]
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Red Clover Mottle Comovirus B-RNA Spreads between Cells in Tobamovirus-infected Tissues
J. Gen. Virol. 69, 407 (1988); https://doi.org/10.1099/0022-1317-69-2-407
/content/journal/jgv/10.1099/0022-1317-69-2-407
/content/journal/jgv/10.1099/0022-1317-69-2-407
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