Recombinant plasmids containing dimeric inserts of tomato golden mosaic virus (TGMV) DNA component A (pA2), DNA component B (pB2) and both DNA components (pA2B2) were constructed. When inoculated onto plants, mixtures of uncut pA2 and pB2, or uncut pA2B2 alone, induced symptoms typical of TGMV infection. Infections induced by the uncut dimeric clones were very similar to those induced by the excised monomeric clones, as judged by symptom development, production of capsid protein, immunological reaction with antiserum to TGMV, production of circular double-stranded DNA and single-stranded DNA of genome size, and restriction analysis of supercoiled DNA. Infection with the dimeric DNA clones gave rise to a subgenomic DNA, derived from DNA A, which was not observed in infections with monomeric clones. The infectivity of excised monomeric DNA was shown to be dependent on the cloning site, but the uncut dimeric clones were as infectious as the most infectious monomeric clones.


Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error