1887

Abstract

Summary

Several retroviruses encode -acting factors which activate gene expression directed by long terminal repeat (LTR) sequences and play a role in the positive feedback regulation of virus replication. We have examined two Mason-Pfizer monkey virus (MPMV) strains for their ability to produce and respond to such factors. Plasmids with the LTR of either MPMV or type D retrovirus/New England (D/NE) were fused to the bacterial chloramphenicol acetyltransferase (CAT) gene. Introduction of these plasmids into several different human cell lines gave rise to significant CAT activity, demonstrating the strong transcriptional promoter activity of these LTRs. However, little or no increase in CAT activity was found upon transfection of these plasmids into MPMV-or D/NE-infected cell lines as compared with uninfected cell lines. Furthermore, CAT activity was not enhanced in uninfected cells by cotransfecting either a functional MPMV DNA clone, a plasmid expressing the human T-lymphotropic retrovirus -activator genes, -1 or -3. These data show that the property of -activation of LTR-mediated gene expression is a function in the replication of only certain retroviruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-8-2265
1987-08-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/8/JV0680082265.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-8-2265&mimeType=html&fmt=ahah

References

  1. Arya S. K., Guo C., Josephs S. F., Wong-Staal F. 1985; Trans-3.ctiv3.tor gene of human T-lymphotropic virus type III (HTLV-III). Science 229:69–73
    [Google Scholar]
  2. Barker C. S., Pickel J., Tainsky M., Hunter E. 1986; Molecular cloning of the Mason-Pfizer monkey virus genome: biological characterization of genome length clones and molecular comparisons to other retroviruses. Virology 153:201–214
    [Google Scholar]
  3. Broome S., Gelbert W. 1985; Rous sarcoma virus encodes a transcriptional activator. Cell 40:537–546
    [Google Scholar]
  4. Cann A. J., Rosenblatt J. D., Wachsman W., Shah N. P., Chen I. S. Y. 1985; Identification of the gene responsible for human T cell leukaemia virus transcriptional regulation. Nature; London: 318517–519
    [Google Scholar]
  5. Chen I. S. Y., Slamon D. J., Rosenblatt J. D., Shah N. P., Quan S. R., Wachsman W. 1985; The X gene IS essential for HTLV replication. Science 229:54–58
    [Google Scholar]
  6. Chopra H. C., Mason M. M. 1970; A new virus in a spontaneous mammary tumour of a rhesus monkey. Cancer Research 30:3081–3086
    [Google Scholar]
  7. Cullen B. R. 1986; Trans-activation of human immunodeficiency virus occurs via a bimodal mechanism. Cell 46:973–982
    [Google Scholar]
  8. Dayton A. I., Sodroski J. G., Rosen C. A., Goh W. C., Haseltine W. A. 1986; The tram-activator gene of the human T cell lymphotropic virus type III is required for replication. Cell 44:941–945
    [Google Scholar]
  9. Desrosiers R. C., Daniel M. D., Butler C. V., Schmidt D. K., Letvin N. L., Hunt R. D., King N. W., Barker C. S., Hunter E. 1985; Retrovirus D/New England and its relation to Mason-Pfizer monkey virus. Journal of Virology 54:552–560
    [Google Scholar]
  10. Dynan W. S., Than R. 1985; Control of eukaryotic messenger RNA synthesis by sequence-specific DNA- binding proteins. Nature; London: 316774–778
    [Google Scholar]
  11. Felber B. K., Paskalis H., Kleinman-Ewing C., Wong-Staal F., Pavlakis G. 1985; The pX protein of human T-cell leukemia virus-I is a transcriptional activator of its long terminal repeats. Science 229:675–679
    [Google Scholar]
  12. Fisher A. G., Feinberg M. B., Josephs S. F., Harper M. E., Marselle L. M., Reyes G., Gonda M. A., Aldovini A., Debouk C., Gallo R. C., Wong-Staal F. 1986; The tram-activator gene of HTLV-III is essential for virus replication. Nature; London: 320367–371
    [Google Scholar]
  13. Gorman C. M., Merlino G. T., Willingham M. C., Pastan I., Howard B. H. 1982a; The RoUS sarcoma virus long terminal repeat is a strong promoter when introduced into a variety of eukaryotic cells by DNA- mediated transfection. Proceedings of the National Academy of Sciences U.S.A.: 796777–6781
    [Google Scholar]
  14. Gorman C. M., Moffat L. F., Howard B. H. 1982b; Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells. Molecular and Cellular Biology 2:1044–1051
    [Google Scholar]
  15. Hess J. L., Clements J. E., Narayan O. 1986; Cis- and tram-acting transcriptional regulation of visna virus. Science 229:482–485
    [Google Scholar]
  16. Mcknight S., Tjian R. 1986; Transcriptional selectivity of viral genes in mammalian cells. Cell 46:795–805
    [Google Scholar]
  17. Majors J., Varmus H. E. 1983; A small region of the mouse mammary tumor virus long terminal repeat confers glucocorticoid hormone regulation on a linked heterologous gene. Proceedings of the National Academy of Sciences U.S.A.: 805866–5870
    [Google Scholar]
  18. Marx P. A., Pedersen N. C., Lerche N. W., Osborn K. G., Lowenstine L. G., Lackner A. A., Maul D. H., Kwang H.-S., Kluge J. D., Zaiss C. P., Sharpe V., Spinner A. P., Allison A. C., Gardner M. B. 1986; Prevention of simian acquired immune deficiency syndrome with a formalin-inactivated type D retrovirus vaccine. Journal of Virology 60:431–435
    [Google Scholar]
  19. Okamoto T., Wong-Staal F. 1986; Demonstration of virus-specific transcriptional activator(s) in cells infected with HTLV-III by an in vitro cell-free system. Cell 47:29–35
    [Google Scholar]
  20. Power M. D., Marx P. A., Bryant M. L., Gardner M. B., Barr P. J., Luciw P. 1986; Nucleotide sequence of SRV-1, a type D acquired immune deficiency syndrome retrovirus. Science 231:1567–1572
    [Google Scholar]
  21. Queen C., Baltimore D. 1983; Immunoglobulin gene transcription is activated by downstream sequence elements. Cell 33:741–748
    [Google Scholar]
  22. Rey M. A., Spire B., Dormont D., Barre-Sinoussi F., Montagnier L., Chermann J.-C. 1984; Characterization of the RNA dependent DNA polymerase of a new human T lymphotropic retrovirus (lymphadenopathy associated virus). Biochemical and Biophysical Research Communications 121:126–133
    [Google Scholar]
  23. Rho H. M., Poiesz B., Ruscetti F. W., Gallo R. C. 1981; Characterization of the reverse transcriptase from a new retrovirus (HTLV) produced by a human cutaneous T-cell lymphoma cell line. Virology 112:355–360
    [Google Scholar]
  24. Rosen C. A., Sodroski J. G., Kettman R., Burny A., Haseltihe W. A. 1985; Tram-activation of the bovine leukemia virus long terminal repeat in infected cells. Science 227:320–322
    [Google Scholar]
  25. Rosen C. A., Sodroski J. G., Goh W. C., Dayton A. I., Lippe J., Haseltine W. A. 1986; Post-transcriptional regulation accounts for the trans- activation of the human T-lymphotropic virus type III. Nature; London: 319555–559
    [Google Scholar]
  26. Seigel L. J., Ratner L., Josephs S. F., Derse D., Feinberg M. B., Reyes G. R., O’Brien S. J., Wong-Staal F. 1986; Tram-activation induced by human lymphotropic virus type III (HTLV-III) maps to a viral sequence encoding 58 amino acids and lacks tissue specificity. Virology 148:226–231
    [Google Scholar]
  27. Seiki M., Inoue J., Takeda T., Yoshida M. 1986; Direct evidence that p40x of human T-cell leukemia virus type I is a tram-acting transcriptional activator. EMBO Journal 5:561–565
    [Google Scholar]
  28. Sodroski J. G., Rosen C., Haseltine W. A. 1984; Tram-acting transcriptional activator of the long terminal repeat of human T lymphotropic viruses in infected cells. Science 225:381–385
    [Google Scholar]
  29. Sodroski J., Patarca R., Rosen C., Haseltine W. A. 1985a; Location of the tram-activating region on the genome of human T-cell lymphotropic virus type III. Science 229:74–77
    [Google Scholar]
  30. Sodroski J. G., Rosen C. A., Wong-Staal F., Salahuddin S. Z., Popovic M., Arya S., Gallo R. C., Haseltine W. A. 1985b; Tram-acting transcriptional regulation of human T-cell leukemia virus type III long terminal repeat. Science 227:171–173
    [Google Scholar]
  31. Sodroski J. G., Rosen C. A., Goh W. C., Haseltine W. A. 1985c; A transcriptional activator protein encoded by the x-lor region of the human T-cell leukemia virus. Science 228:1430–1434
    [Google Scholar]
  32. Sonigo P., Barker C., Hunter E., Wain-Hobson S. 1986; Nucleotide sequence of Mason-Pfizer monkey virus: an immunosuppressive D-type retrovirus. Cell 45:375–385
    [Google Scholar]
  33. Southern P. J., Berg P. 1982; Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter. Journal of Molecular and Applied Genetics 1:327–341
    [Google Scholar]
  34. Stromberg K., Benveniste R. E., Arthur L. O., Rabin H., Giddens W. E., Ochs H. D., Morton W. R., Tsai C.-C. 1984; Characterization of exogenous type D retrovirus from a fibroma of a macaque with simian AIDS and fibromatosis. Science 224:289–292
    [Google Scholar]
  35. Van Der Eb A. J., Graham F. L. 1980; Assay of transforming activity of tumor virus DNA. Methods in Enzymology 65:826–839
    [Google Scholar]
  36. Wright C. M., Felber B. K., Paskalis H., Pavlakis G. N. 1986; Expression and characterization of the trans- activator of the HTLV-III virus. Science 234:988–992
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-8-2265
Loading
/content/journal/jgv/10.1099/0022-1317-68-8-2265
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error