1887

Abstract

Summary

The surface structure of foot-and-mouth disease virus (FMDV) and the interaction of the individual capsid proteins with the virus RNA have been examined using modification reagents. By measuring the extent of modification of the lysine residues of intact and disrupted virus particles and the 12S protein subunit with Bolton & Hunter reagent it was found that 54 of the residues of VP 1, 15 % of the residues of VP2 and 37 % of the residues of VP3, equivalent to five, two and four lysine residues respectively, are on the surface of the intact virus particle. Polypeptide VP4 was not modified in intact virus particles, indicating that it has no lysine residues on the surface of the virus. Modification with sodium metabisulphite, which causes a specific transamination reaction between cytidylic acid residues in ssRNA and closely associated basic amino acids, cross-linked all four structural proteins to the virus RNA. Both fragments of VP1, produced by treatment of the virus particle with trypsin, are also cross-linked to the RNA. These observations have been combined with the evidence that the immunogenic activity of VP1 may be contained in two discontinuous sites, at amino acids 141 to 160 and 200 to 213, in proposing a model for the arrangement of this polypeptide in the virus particle.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-6-1649
1987-06-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/6/JV0680061649.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-6-1649&mimeType=html&fmt=ahah

References

  1. Bachrach H. L. 1968; Foot-and-mouth disease. Annual Review of Microbiology 22:201–224
    [Google Scholar]
  2. Beneke T. W., Habermehl K.-O., Diefenthal W., Buchholz M. 1977; Iodination of poliovirus capsid proteins. Journal of General Virology 34:387–390
    [Google Scholar]
  3. Bittle J. L., Houghton R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A., Rowlands D. J., Brown F. 1982; Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature; London: 29830–33
    [Google Scholar]
  4. Bolton A. E. 1973; The labelling of proteins to high specific radioactivity by conjugation to a [125I]-containing acylating reagent. Biochemical Journal 133:529–539
    [Google Scholar]
  5. Boothroyd J. C., Harris T. J. R., Rowlands D. J., Lowe P. A. 1982; The nucleotide sequence of cDNA coding for the structural proteins of FMDV. Gene 17:153–161
    [Google Scholar]
  6. Brown F., Cartwright B. 1963; Purification of radioactive foot-and-mouth disease virus. Nature; London: 1991168–1170
    [Google Scholar]
  7. Burroughs J. N., Rowlands D. J., Sangar D. V., Talbot P., Brown F. 1971; Further evidence for multiple proteins in the foot-and-mouth disease virus particle. Journal of General Virology 13:73–84
    [Google Scholar]
  8. Cartwright B., Morrell D. J., Brown F. 1982; Nature of the antibody response to the foot-and-mouth disease virus particle, its 12S protein subunit and the isolated immunizing polypeptide, VP1. Journal of General Virology 63:375–381
    [Google Scholar]
  9. Cavanagh D., Sangar D. V., Rowlands D. J., Brown F. 1977; Immunogenic and cell attachment sites of FMDV : further evidence for their location in a single capsid polypeptide. Journal of General Virology 35:149–158
    [Google Scholar]
  10. Chou P. Y., Fasman P. D. 1974; Prediction of protein conformation. Biochemistry 13:222–224
    [Google Scholar]
  11. Cooper P. D., Agol V. I., Bachrach H. L., Brown F., Ghendon Y., Gibbs A. J., Gillespie J. H., Lonberg-Holm K., Mandel B., Melnick I. L., Mohanty S. B., Povey R. C., Rueckert R. R., Schaffer F. L., Tyrrell D. A. J. 1978; Picornaviridae: second report. Intervirology 10:165–180
    [Google Scholar]
  12. Denoya C. D., Scodeller E. A., Gimenez B. H., Vasquez C., Latorre J. L. 1978a; Foot-and-mouth disease virus. I. Stability of its ribonucleic acid. Virology 84:230–235
    [Google Scholar]
  13. Denoya C. D., Scodeller E. A., Vasquez C., La Torre J. L. 1978b; Foot-and-mouth disease virus. II. Endoribonuclease activity within purified virions. Virology 89:67–74
    [Google Scholar]
  14. Dimarchi R., Brooke G., Gale C., Cracknell V., Doel T., Mowat N. 1986; Protection of cattle against foot- and-mouth disease by a synthetic peptide. Science 232:639–641
    [Google Scholar]
  15. Ehresmann B., Briand J.-P., Reinbolt J., Witz J. 1980; Identification of binding sites of turnip yellow mosaic virus protein and RNA by cross-links induced in situ . European Journal of Biochemistry 108:123–129
    [Google Scholar]
  16. Garoff H., Frischauf A.-M., Simons K., Larach H., Delius H. 1980; The capsid protein of Semliki Forest virus has clusters of basic amino acids and prolines in its amino-terminal region. Proceedings of the National Academy of Sciences U.S.A.: 776376–6380
    [Google Scholar]
  17. Glazer A. N. 1982; The chemical modification of proteins by group-specific and site-specific reagents. In The Proteins vol5, 3rd edn. pp 1–103 Neurath H., Hill R. L. Edited by New York: Academic Press;
    [Google Scholar]
  18. Harris T. J. R., Brown F. 1977; Biochemical analysis of a virulent and an avirulent strain of foot-and-mouth disease virus. Journal of General Virology 34:87–105
    [Google Scholar]
  19. Harrison S. C., Olson A.J, Vaara I., Kaman K, Fridberg K. 1980; Tomato bushy Stunt virus at 2.9A resolution. Nature; London: 276368–373
    [Google Scholar]
  20. Hogle J. M., Chow M., Filman D. J. 1985; Three dimensional structure of poliovirus at 2.9Å. Science 229:1358–1365
    [Google Scholar]
  21. Hopp T. P., Woods K. R. 1981; Prediction of protein antigenic determinants from amino acid sequences. Proceedings of the National Academy of Sciences U.S.A.: 783824–3828
    [Google Scholar]
  22. Jacrot B., Chauvin C., Witz J. 1977; Comparative neutron small-angle scattering study of small spherical RNA viruses. Nature; London: 266417–421
    [Google Scholar]
  23. King A. M. Q., Newman J. W. I. 1980; Temperature sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. I. Identification by electrofocusing. Journal of Virology 34:59–66
    [Google Scholar]
  24. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  25. Laporte J., Lenoir G. 1973; Structural proteins of foot-and-mouth disease virus. Journal of General Virology 20:161–168
    [Google Scholar]
  26. Laporte J., Grosclaude J., Wantyghem J., Bernard S. 1973; Neutralisation en culture cellulaire du pouvoir infectieux du virus de la fièvre aphteuse des sérums provenant de pores immunisés à l’aide d’une protein virale purifiée. Compte rendu hebdomadaire des Séances de I’Académie des sciences 276D:3399–3402
    [Google Scholar]
  27. Lonberg-Holm K., Butterworth B. E. 1976; Investigation of the structure of polio- and rhinovirions through the use of selective chemical reactivity. Virology 78:207–216
    [Google Scholar]
  28. Lund C. A., Ziola B. R., Salmi A., Scraba D. G. 1977; Structure of the Mengo virion. V. Distribution of the capsid polypeptides with respect to the surface of the virus particle. Virology 78:35–44
    [Google Scholar]
  29. Morrell D. J. 1983 Studies on the structure and immunogenicity of foot-and-mouth disease virus Ph.D. thesis University of Reading:
    [Google Scholar]
  30. Nomoto A., Omata T., Toyoda H., Kuge S., Horrie H., Kataoka Y., Genba Y., Nahano Y., Imura N. 1982; Complete nucleotide sequence of the attenuated poliovirus Sabin I strain genome. Proceedings of the National Academy of Sciences U.S.A.: 795793–5797
    [Google Scholar]
  31. Palmenberg A. C., Kirby E. W., Janda M. R., Drake M. L., Duke G. M., Poratz K. F., Collet M. S. 1984; The nucleotide and deduced amino acid sequence of the encephalomyocarditis viral protein coding region. Nucleic Acids Research 12:2969–2985
    [Google Scholar]
  32. Parry N., Ouldridge E., Barnett P., Rowlands D. J., Brown F., Bittle J., Houghten R., Lerner R. A. 1985; Identification of neutralizing epitopes of foot-and-mouth disease virus. In Vaccines 85 pp 211–216 Lemer R. A., Chanock M., Brown F. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  33. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H.-J., Johnson J. E., Kamer G., Lou M., Mosser A. G., Rueckert R. R., Sherry B., Vriend G. 1985; Structure of a human common cold virus and functional relationship to other picomaviruses. Nature; London: 317145–153
    [Google Scholar]
  34. Rowlands D. J., Sangar D. V., Brown F. 1975; A comparative chemical and serological study of the full and empty particles of foot-and-mouth disease virus. Journal of General Virology 26:227–238
    [Google Scholar]
  35. Sangar D. V., Rowlands D. J., Cavanagh D., Brown F. 1976; Characterization of the minor polypeptides in the foot-and-mouth disease virus particle. Journal of General Virology 31:35–46
    [Google Scholar]
  36. Shapiro R., Gasit G. 1977; Cross-linking of nucleic acids and proteins by bisulphite. In Protein Cross-linking: Biochemical and Molecular Aspects. Advances in Expeimental Medicine and Biology pp 633–640 Friedman M. Edited by New York: Plenum Press;
    [Google Scholar]
  37. Strohmaier K., Franze R., Adam K.-H. 1982; Location and characterization of the antigenic portion of the FMDV immunizing protein. Journal of General Virology 59:295–306
    [Google Scholar]
  38. Talbot P., Rowlands D. J., Burroughs J. N., Sangar D. V., Brown F. 1973; Evidence for a group protein in foot-and-mouth disease virus particles. Journal of General Virology 19:369–380
    [Google Scholar]
  39. Vande Woude C. G., Swaney J. B., Bachrach H. L. 1972; Chemical and physical properties of foot-and-mouth disease virus: a comparison with Maus-Eberfeld virus. Biochemical and Biophysical Research Communications 48:1222–1229
    [Google Scholar]
  40. Vasquez C., Denoya C. D., La Torre J. L., Palma E. L. 1979; Structure of foot-and-mouth disease virus capsid. Virology 97:195–200
    [Google Scholar]
  41. Wetz K., Habermehl K.-O. 1979; Topographical studies on poliovirus capsid proteins by chemical modification and cross-linking with bifunctional reagents. Journal of General Virology 44:525–534
    [Google Scholar]
  42. Wetz K., Habermehl K.-O. 1982; Specific cross-linking of capsid proteins to virus RNA by ultraviolet irradiation of poliovirus. Journal of General Virology 59:397–401
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-6-1649
Loading
/content/journal/jgv/10.1099/0022-1317-68-6-1649
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error