1887

Abstract

Summary

DNA representing RNA segment 3 of bluetongue virus (BTV) serotype 17, corresponding to the gene that codes for a group-specific antigen VP3, has been inserted into a baculovirus transfer vector in lieu of the 5′ coding region of the polyhedrin gene of nuclear polyhedrosis virus (AcNPV). After cotransfection of cells with wild-type AcNPV DNA in the presence of the derived recombinant transfer vector DNA, polyhedrin-negative recombinant baculoviruses were recovered. When cells were infected with one of these recombinant viruses, a protein that was similar in size and antigenic properties to the BTV VP3 protein was synthesized. Antibodies raised in mice or rabbits to the baculovirus-expressed VP3 protein immunoprecipitated the VP3 protein of BTV-17 as well as that of BTV-10. The expressed antigen reacted with antisera representing four U.S.A. BTV serotypes in an indirect ELISA test.

Keyword(s): antigen VP3 , baculovirus and BTV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-6-1627
1987-06-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/6/JV0680061627.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-6-1627&mimeType=html&fmt=ahah

References

  1. Brown M., Faulkner P. 1977; A plaque assay for nuclear polyhedrosis viruses using a solid overlay. Journal of General Virology 36:361–364
    [Google Scholar]
  2. Denhardt D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  3. Ghiasi H., Purdy M. A., Roy P. 1985; The complete sequence of bluetongue virus serotype 10 segment 3 and its predicted VP3 polypeptide compared with those of BTV serotype 17. Virus Research 3:181–190
    [Google Scholar]
  4. Gorman B. M., Taylor J., Walker P. J. 1983; Orbiviruses. In The Reoviridae pp 287–357 Joklik W. K. Edited by London: Plenum;
    [Google Scholar]
  5. Hooft Van Iddenkinge B. J. L., Smith G. E., Summers M. D. 1983; Nucleotide sequence of the polyhedrin gene of Autographa californica nuclear polyhedrosis virus. Virology 131:561–565
    [Google Scholar]
  6. Howard S. C., Ayres M. D., Possee R. D. 1986; Mapping the 5′ and 3′ ends of Autographa californica polyhedrin mRNA. Virus Research 5:109–119
    [Google Scholar]
  7. Huismans H. 1979; Protein synthesis in bluetongue virus-infected cells. Virology 92:385–396
    [Google Scholar]
  8. Huismans H., Erasmus B. J. 1981; Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort Journal of Veterinary Research 48:51–58
    [Google Scholar]
  9. Huismans H., Bremer C. W., Barber T. L. 1979; The nucleic acid and proteins of epizootic haemorrhagic disease virus. Onderstepoort Journal of Veterinary Research 46:95–104
    [Google Scholar]
  10. Inumaru S., Roy P. 1987; Production and characterization of the neutralization antigen VP2 of bluetongue virus serotype 10. Virology 157:472–479
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 277680–685
    [Google Scholar]
  12. Martin S. A., Zweerink H. J. 1972; Isolation and characterization of two types of bluetongue virus particles. Virology 50:495–506
    [Google Scholar]
  13. Matsuura Y., Possee R. D., Bishop D. H. L. 1986; Expression of the S-coded genes of lymphocytic choriomeningitis arenavirus using a baculovirus vector. Journal of General Virology 67:1515–1529
    [Google Scholar]
  14. Maxam A. M., Gilbert W. 1980; Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  15. Mertens P. P. C., Brown F., Sangar D. V. 1984; Assignment of the genome segments of bluetongue virus type 1 to the proteins which they encode. Virology 135:207–217
    [Google Scholar]
  16. Possee R. D. 1986; Cell-surface expression of influenza virus haemagglutinin in insect cells using a baculovirus vector. Virus Research 5:43–49
    [Google Scholar]
  17. Purdy M. A., Petre J., Roy P. 1984; Cloning of the bluetongue virus L3 gene. Journal of Virology 51:754–759
    [Google Scholar]
  18. Roy P., Ritter G. D. Jr Akashi H., Collisson E., Inaba Y. A. 1985; A genetic probe for identifying bluetongue virus infections in vivo and in vitro . Journal of General Virology 66:1613–1619
    [Google Scholar]
  19. Sangar D. V., Mertens P. P. C. 1983; Comparisons of type 1 bluetongue virus protein synthesis in vivo and in vitro . In Double-Stranded RNA Viruses pp 183–191 Compans R. W., Bishop D. H. L. Edited by New York: Elsevier;
    [Google Scholar]
  20. Smith G. E., Summers M. D., Fraser M. J. 1983; Production of human beta interferon in insect cells infected with a baculovirus expression vector. Molecular and Cellular Biology 3:2156–2165
    [Google Scholar]
  21. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–518
    [Google Scholar]
  22. Vlak J. M., Smith G. E., Summers M. D. 1981; Hybridization selection and in vitro translation of Autographa californica nuclear polyhedrosis virus mRNA. Journal of Virology 40:762–771
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-6-1627
Loading
/content/journal/jgv/10.1099/0022-1317-68-6-1627
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error