1887

Abstract

SUMMARY

Hybridomas producing human monoclonal antibodies (MAbs) against human cytomegalovirus (CMV) were generated by fusion of human spleen cells and mouse myeloma cells. Two of the six MAbs obtained neutralized viral infectivity even at concentrations lower than 1 μg/ml. One MAb required complement for neutralization but the other did not. Both MAbs recognized viral proteins of M 130000 and 55000. Furthermore, these neutralizing MAbs bound to the surface membrane of CMV- infected cells. These results suggest that human MAbs may provide a new means of passive immunization against CMV infection in humans.

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/content/journal/jgv/10.1099/0022-1317-68-5-1457
1987-05-01
2022-08-17
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References

  1. Britt W. J., Auger D. 1986; Synthesis and processing of the envelope gp55-l 16 complex of human cytomegalovirus. Journal of Virology 58:185–191
    [Google Scholar]
  2. Burnett K. G., Masuho Y., Hernandez R., Maeda T., King M. P., Martinis J. 1985; Human monoclonal antibodies to breast tumor cells. In Monoclonal Antibodies: Diagnosis and Therapeutic Use in Tumor and Transplantation pp. 47–61 Chatterjee S. N. Edited by Littleton: PSG Publishing Co. Ltd;
    [Google Scholar]
  3. Cole S. p. c., Campling B. G., Atlaw T., Kozbor D., Roder J. C. 1984; Human monoclonal antibodies. Molecular and Cellular Biochemistry 62:109–120
    [Google Scholar]
  4. Condie R. M., O’Reilley R. J. 1984; Prevention of cytomegalovirus infection by prophylaxis with an intravenous, hyperimmune, native, unmodified cytomegalovirus globulin. Randomized trial in bone marrow transplant recipients. American Journal of Medicine 76(3A):134–141
    [Google Scholar]
  5. Farrar G. H., Oram J. D. 1984; Characterization of the human cytomegalovirus envelope glycoproteins. Journal of General Virology 65:1991–2001
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 221680–685
    [Google Scholar]
  7. Kettering J. D., Schmidt N. j., Galls D., Lennette E. H. 1977; Anti-complement immunofluorescent test for antibodies to human cytomegalovirus. Journal of Clinical Microbiology 6:627–632
    [Google Scholar]
  8. Masuho Y., Sugano T., Matsumoto Y., Sawada S., Tomibe K. 1986; Generation of hybridomas producing human monoclonal antibodies against herpes simplex virus after in vitro stimulation. Biochemical and Biophysical Research Communications 135:495–500
    [Google Scholar]
  9. Matsumoto Y., Sugano T., Miyamoto C., Masuho Y. 1986; Establishment of hybridomas producing human monoclonal antibodies against human cytomegalovirus. Biochemical and Biophysical Research Communications 137:273–280
    [Google Scholar]
  10. Meyers J. D., Flournoy N., Wade J. C., Hackman R. C., Mcdougall J. K., Neiman P. E., Thomas E. D. 1983; Biology of interstitial pneumonia after marrow transplantation. In Recent Advances in Bone Marrow Transplantation pp. 405–423 Gale R. P. Edited by New York: Alan R. Liss Inc;
    [Google Scholar]
  11. Pereira L. 1984; Glycoproteins specified by human cytomegalovirus. In The Herpesviruses 3 pp. 383–404 Roizman B. Edited by New York: Plenum Press;
    [Google Scholar]
  12. Quinnan G. V., Rook A. H., Frederick W. R., Manischewitz J. F., Epstein J. S., Siegel J., Masur H., Macher A. M., Mitchell C., Armstrong G., Djeu J. Y. 1984; Prevalence, clinical manifestations, and immunology of herpesvirus infections in the acquired immunodeficiency syndrome. Annals of the New York Academy of Sciences 437:200–206
    [Google Scholar]
  13. Rasmussen L. E., Nelson R. M., Kelsall N. D., Merigan T. C. 1984; Murine monoclonal antibody to a single protein neutralizes the infecti vity of human cytomegalovirus. Proceedings of the National Academy of Sciences, U.S.A 81:876–880
    [Google Scholar]
  14. Rasmussen L., Mullenax J., Nelson R., Merigan T. C. 1985; Viral polypeptides detected by a complement-dependent neutralizing murine monoclonal antibody to human cytomegalovirus. Journal of Virology 55:274–280
    [Google Scholar]
  15. Redmond M. J., Leyritz-Wills M., Winger L., Scraba D. G. 1986; The selection and characterization of human monoclonal antibodies to human cytomegalovirus. Journal of Virological Methods 14:9–24
    [Google Scholar]
  16. Sawada S., Kawamura T., Masuho Y., Tomibe K. 1985; Characterization of a human monoclonal antibody to lipopolysaccharides of Pseudomonas aeruginosa serotype 5: a possible candidate as an immunotherapeutic agent for infections with P. aeruginosa . Journal of Infectious Diseases 152:965–970
    [Google Scholar]
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