1887

Abstract

Summary

Time-resolved fluoroimmunoassay (TR-FIA) was used to detect potato virus Y in potato leaf extracts diluted at least 10-fold, and in extracts of secondarily infected dormant tubers diluted 10- or 10-fold depending on the virus strain used. TR-FIA was also used to detect potato virus X in extracts of secondarily infected tubers diluted up to 10-fold. The response in the TR-FIA using a two-incubation method was linearly related to the virus concentration in a series of dilutions and the method can therefore be used for quantitative analysis. TR-FIA was found to be five to 100 times more sensitive than conventional double antibody sandwich ELISA.

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1987-05-01
2022-01-20
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References

  1. Banttari E. E., Franc G. D. 1982; Enzyme-linked immunosorbent assay with single or combined antisera for viruses S and X in potato tubers and plants. American Potato Journal 59:375–387
    [Google Scholar]
  2. Barbara D. J., Clark M. F. 1982; Simple indirect ELISA using F(ab′)2 fragments of immunoglobulin. Annals of Applied Biology 58:315–322
    [Google Scholar]
  3. Bar-Joseph M., Malinkson M. 1980; Hen egg yolk as a source of antiviral antibodies in the enzyme-linked immunosorbent assay (ELISA): a comparison of two plant viruses. Journal ofVirological Methods 1:179–183
    [Google Scholar]
  4. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  5. Daniel G., Hunnius W. 1980; Nachweis der Kartoffelviren M, S, X und Y in Presssäften sekundärinfizierten Kartolfelpflanzen mit ELISA (enzyme-linked immunosorbent assay). Gesunde Pflanzen 32:118–127
    [Google Scholar]
  6. De Bokx J. A., Piron P. G., Cother E. 1980; Enzyme-linked immunosorbent assay (ELISA) for the detection of potato viruses S and M in potato tubers. Netherlands Journal of Plant Pathology 86:285–290
    [Google Scholar]
  7. Hemmilä I., Dakubu S., Mukkala V.-M., Siitari H., Lövgren T. 1984; Europium as a label in time-resolved immunofluorometric assays. Analytical Biochemistry 137:335–343
    [Google Scholar]
  8. Hill S. A., Jackson E. A. 1984; An investigation of the reliability of ELISA as a practical test for detecting potato leaf roll virus and potato virus Y in tubers. Plant Pathology 33:21–26
    [Google Scholar]
  9. Kurppa A. 1983; Potato viruses in Finland and their identification. Journal of the Scientific Agricultural Society of Finland 55:183–301
    [Google Scholar]
  10. Kurppa A., Korhonen K. 1984; ELISA reagents for potato virus Y strains with significantly low non-specific reactions. Journal of Agricultural Science of Finland 56:89–95
    [Google Scholar]
  11. Lommel S. A., McCain A. H., Morris T. J. 1982; Evaluation of indirect enzyme-linked immunosorbent assay for the detection of plant viruses. Phytopathology 72:1018–1022
    [Google Scholar]
  12. Maat D. Z., De Bokx J. A. 1978; Enzyme-linked immunosorbent assay (ELISA) for the detection of potato viruses A and Y in potato leaves and sprouts. Netherlands Journal of Plant Pathology 84:167–174
    [Google Scholar]
  13. Mikola H., Mukkala V. -M., Hemmilä I. 1984; Chelating compound. International Patent ApplicationPLT/WO 84/03698
    [Google Scholar]
  14. Siitari H., Hemmilä I., Soini E., Lövgren T., Koistinen V. 1983; Detection of hepatitis B surface antigen using time-resolved fluoroimmunoassay. Nature; London: 301258–260
    [Google Scholar]
  15. Siitari H., Lövgren T., Halonen P. 1986; Detection of viral antigens by direct one-incubation time-resolved fluoroimmunoassay. In Developments in Applied Biology: Developments and Applications in Virus Testing 1 pp. 155–164 Jones R. A. C., Torrance L. Edited by Wellesbourne: Association of Applied Biologists;
    [Google Scholar]
  16. Soini E., Kojola H. 1983; Time-resolved fluorometer for lanthanide chelates - a new generation of nonisotopic immunoassays. Clinical Chemistry 29:65–68
    [Google Scholar]
  17. Torrance L. 1981; Use of Clq enzyme-linked immunosorbent assay to detect plant viruses and their serologically different strains. Annals of Applied Biology 99:291–299
    [Google Scholar]
  18. Torrance L., Jones R. A. c. 1982; Increased sensitivity of detection of plant viruses obtained by using a fluorogenic substrate in enzyme-linked immunosorbent assay. Annals of Applied Biology 101:501–509
    [Google Scholar]
  19. Van Regenmortel M. H. v., Burckard J. 1980; Detection of a wide spectrum of tobacco mosaic virus strains by indirect enzyme-linked immunosorbent assays (ELISA). Virology 106:327–334
    [Google Scholar]
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