1887

Abstract

SUMMARY

Antigenic interrelationships among paramyxoviruses were examined by immunoprecipitation of isotope-labelled virus-infected cell lysates with specific antisera against virions or virus components. Sendai virus, human parainfluenza virus type 1 (Pa-1) and human parainfluenza virus type 3 (Pa-3) belonged to one antigenic group, and human parainfluenza virus type 2 (Pa-2), human parainfluenza virus type 4 (Pa-4), mumps virus (MuV) and simian virus 5 to a second group. Furthermore, the human paramyxoviruses Pa-1, Pa-2, Pa-3, Pa-4 and MuV formed a single antigenic group which overlapped the above groups. Although Newcastle disease virus (NDV) belonged to a separate group it showed some cross-reaction with the human paramyxoviruses. In particular, certain batches of anti-NDV antisera reacted with the fusion (F) polypeptide of Pa-2 and the reciprocal reaction of anti-Pa-2 antiserum with NDV F was also found. The nucleoprotein showed the broadest cross-reactions among these paramyxoviruses, whereas the matrix polypeptide exhibited antigenic individuality. The nucleoprotein of MuV was most cross-reactive. Pa-2 haemagglutinin- neuraminidase (HN) and anti-MuV HN serum showed cross-reactivity with various antisera and antigens.

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/content/journal/jgv/10.1099/0022-1317-68-5-1289
1987-05-01
2022-11-28
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References

  1. Cook M. K., Andrews B. E., Fox H. H., Turner H. C., James W. D., Chanock R. M. 1959; Antigenic relationships among the “newer” myxoviruses (parainfluenza). American Journal of Hygiene 69:250–264
    [Google Scholar]
  2. Elango N., Coligan J. E., Jambou R. c., Venkatesan S. 1986; Human parainfluenza type 3 virus hemagglutinin-neuraminidase glycoprotein: nucleotide sequence of mRNA and limited amino acid sequence of the purified protein. Journal of Virology 57:481–489
    [Google Scholar]
  3. Goswami K. K. A., Russell W. C. 1982; A comparison of paramyxoviruses by immunoprecipitation. Journal of General Virology 60:177–183
    [Google Scholar]
  4. Goswami K. K. A., Russell W. C. 1983; Monoclonal antibodies against human paramyxovirus type 3 and against SV5 virus: preparation and preliminary characterization. Journal of General Virology 64:1663–1672
    [Google Scholar]
  5. Julkunen I. 1984; Serological diagnosis of parainfluenza virus infections by enzyme assay with special emphasis on purity of viral antigens. Journal of Medical Virology 14:177–187
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 221680–685
    [Google Scholar]
  7. Millar N. s., Chambers P., Emmerson P. T. 1986; Nucleotide sequence analysis of the haemagglutinin-neuraminidase gene of Newcastle disease virus. Journal of General Virology 67:1917–1927
    [Google Scholar]
  8. Ray R., Compans R. W. 1986; Monoclonal antibodies reveal extensive antigenic differences between the hemagglutinin-neuraminidase glycoproteins of human and bovine parainfluenza 3 viruses. Virology 148:232–236
    [Google Scholar]
  9. Sanchez A., Banerjee A. K., Furuichi Y., Richardson M. A. 1986; Conserved structures among the nucleocapsid proteins of the paramyxoviridae: complete nucleotide sequence of human parainfluenza virus type 3 NP mRNA. Virology 152:171–180
    [Google Scholar]
  10. Shioda T., Iwasaki K., Shibuta H. 1986; Determination of the complete nucleotide sequence of the Sendai virus genome RNA and the predicted amino acid sequences of the F, HN and L proteins. Nucleic Acids Research 14:1545–1563
    [Google Scholar]
  11. Spriggs M. K., Olmsted R. A., Venkatesan S., Coligan J. E., Collins P. L. 1986; Fusion glycoprotein of human parainfluenza virus type 3: nucleotide sequence of the gene, direct identification of the cleavage-activation site, and comparison with other paramyxoviruses. Virology 152:241–251
    [Google Scholar]
  12. Van Der Veen J., Sonderkamp H. J. A. 1965; Secondary antibody response of guinea pigs to parainfluenza and mumps viruses. Archiv für die gesamte Virusforschung 15:721–734
    [Google Scholar]
  13. Van Wyke Coelingh K. L., Winter C., Murphy B. R. 1985; Antigenic variation in the hemagglutinin- neuraminidase protein of human parainfluenza type 3 virus. Virology 143:569–582
    [Google Scholar]
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