1887

Abstract

SUMMARY

The effect of rabbit anti-rubella virus (RV) serum on the course of RV infection of human lymphoid cells has been examined. The antibody was found to abolish viral replication such that no virus progeny could be detected either extracellularly (after removal of the antiserum) or intracellularly, in the treated cells. Viral protein synthesis was also found to be totally inhibited in the presence of anti-RV but resumed immediately on removal of the antibody block at levels suggesting that viral RNA had been accumulating in the cell. Infectious focus assays indicated that the effect could be explained in part by the restriction by antiviral antibody of cell-to-cell spread in the indicator monolayer. Thus by 48 h when 100 % of lymphoid cells infected with virus alone were capable of forming infectious foci, only 40 to 50 % of cells infected in the presence of antiviral antibody produced foci. However this restriction imposed by antibody did not adequately explain the total inhibition of viral protein synthesis that occurred under these conditions. Pretreatment of RV with excess neutralizing antiserum reduced the virus titre > 99·9 % but did not eliminate infectious virus. The ‘non-neutralized’ fraction (10 to 10 p.f.u./ml) brought about a productive infection with final yields of 10 to 25 % of control titres (> 10 p.f.u./ml). Incubation of virus with both anti-RV and goat anti-rabbit Ig eliminated viral infectivity, indicating that the ‘non-neutralized’ fraction was in the form of infectious immune complexes. The results suggest two major modes of action for anti-RV antibody, one in neutralizing extracellular virus, the second exerted on the infected cells, totally inhibiting viral translation and thus preventing the virus from completing its replicative cycle.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-5-1277
1987-05-01
2022-01-21
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/5/JV0680051277.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-5-1277&mimeType=html&fmt=ahah

References

  1. Cardosa M. J., Porterfield J. S., Gordon S. 1983; Complement receptor mediates enhanced flavivirus replication in macrophages. Journal of Experimental Medicine 158:258–263
    [Google Scholar]
  2. Chantler J. K. 1979; Rubella virus: intracellular polypeptide synthesis. Virology 98:275–278
    [Google Scholar]
  3. Chantler J. K. 1981; Permissiveness of human T and B lymphocytes to rubella virus. Abstracts, Fifth International Congress of Virology (Strasbourg) p. 383
    [Google Scholar]
  4. Chantler J. K., Tingle A. J. 1980; Replication and expression of rubella virus in human lymphocyte populations. Journal of General Virology 50:317–328
    [Google Scholar]
  5. Chantler J. K., Ford D. K., Tingle A. J. 1982; Persistent rubella infection and rubella-associated arthritis. Lancet i:1323–1325
    [Google Scholar]
  6. Cooper L. z., Buimovici-Klein E. 1985; Rubella. In Virology pp. 1005–1020 Fields B. N., Knipe D. M., Chanock R. M., Melnick J. L., Roizman B., Shope R. E. Edited by New York: Raven Press;
    [Google Scholar]
  7. Fujinami R. S., Oldstone M. B. A. 1979; Antiviral antibody reacting on the plasma membrane after measles virus expression inside the cell. Nature; London: 279529–530
    [Google Scholar]
  8. Fujinami R. s., Oldstone M. B. A. 1980; Alterations in expression of measles virus polypeptides by antibody: molecular events in antibody-induced antigenic modulation. Journal of Immunology 125:78–85
    [Google Scholar]
  9. Fujinami R. s., Oldstone M. B. A. 1984; Antibody initiates virus persistence: immune modulation and measles virus infection. In Concepts in Viral Pathogenesis pp. 187–193 Notkins A. L., Oldstone M. B. A. Edited by Wien & New York: Springer-Verlag;
    [Google Scholar]
  10. Gorman N. T., Lachmann P. J. 1982; In vitro modulation of viral cell surface glycoproteins by antiviral antibody in the presence of complement. Clinical and Experimental Immunology 50:507–514
    [Google Scholar]
  11. Halstead S. B., O’Rourke E. J. 1977; Dengue viruses and mononuclear phagocytes. 1. Infection enhancement by non-neutralising antibody. Journal of Experimental Medicine 146:201–217
    [Google Scholar]
  12. Horta-Barbosa L., Hamilton R., Wittig B., Fuccillo D. A., Sever J. L. 1971; Subacute sclerosing panencephalitis: isolation of suppressed measles virus from lymph node biopsies. Science 173:840–841
    [Google Scholar]
  13. Hudson L., Hay F. c. 1980; Isolation and structure of immunoglobulins. In Practical Immunology, 2nd edn. pp. 156–202 Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  14. Kouri G., Aquilera A., Rodriguez P., Korolev M. 1974; A study of microfoci and inclusion bodies produced by rubella virus in the RK-13 cell line. Journal of General Virology 22:73–80
    [Google Scholar]
  15. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  16. Mandel B. 1979; Interactions of viruses with neutralising antibodies. In Comprehensive Virology 15 pp. 37–121 Fraenkel-Conrat H., Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  17. Oldstone M. B. A., Tishan A. 1978; Immunological injury in measles virus infection. IV. Antigenic modulation and abrogation of lymphocyte lysis of virus-infected cells. Clinical Immunology and Immunopathology 9:55–62
    [Google Scholar]
  18. Payne F. E., Baublis J. V., Itabashi H. H. 1969; Isolation of measles virus from cell cultures of brain from a patient with subacute sclerosing panencephalitis. New England Journal of Medicine 281:583–589
    [Google Scholar]
  19. Peiris J. S. M., Porterfield J. S. 1979; Antibody mediated enhancement of flavivirus replication in macrophage-like cell lines. Nature; London: 282509–511
    [Google Scholar]
  20. Perlmann P. 1984; Antibody-dependent cellular cytotoxicity (ADCC) mediated by human killer cells. In Concepts in Viral Pathogenesis pp. 58–64 Notkins A. L., Oldstone M. B. A. Edited by Wien & New York: Springer-Verlag;
    [Google Scholar]
  21. Porterfield J. s., Cardosa M. J. 1985; Host range and tissue tropisms: antibody-dependent mechanisms. In Concepts in Viral Pathogenesis pp. 117–123 Notkins A. L., Oldstone M. B. A. Edited by Wien & New York: Springer-Verlag;
    [Google Scholar]
  22. Wrzos H., Kulczycki J., Laskowski Z., Matacz D., Brzosko W. J. 1979; Detection of measles antigen(s) in peripheral lymphocytes from patients with subacute sclerosing panencephalitis. Archives of Virology 60:291–297
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-5-1277
Loading
/content/journal/jgv/10.1099/0022-1317-68-5-1277
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error