1887

Abstract

Summary

Using both selection enrichment and site-directed mutagenesis, a herpes simplex virus type 1 (HSV-1) strain 17 genome lacking all four I sites has been generated. The site at 0.45 map units which lies within the gene coding for a polypeptide of 28 000 molecular weight was removed by selection enrichment, while the site at 0.29 map units which lies within the gene coding for glycoprotein H was removed by site-directed mutagenesis. The parental virus from which these two I sites were deleted had previously had the sites at 0.07 and 0.63 map units removed through selection enrichment. The variant devoid of I sites (X4) showed normal growth characteristics; its phenotype was normal apart from the absence of the thymidine kinase protein, which is believed to be unrelated to the loss of I sites.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-4-1165
1987-04-01
2019-11-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-4-1165
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error