1887

Abstract

SUMMARY

Prostaglandin J (PGJ) was found to suppress dramatically Sendai virus replication in African green monkey kidney cells in culture. PGJ was not toxic at the active dose to uninfected cells and did not significantly inhibit macromolecular synthesis, but it specifically stimulated the synthesis of a polypeptide of 74000 mol. wt. In Sendai virus-infected cells, PGJ partially inhibited virus protein synthesis and caused an alteration in the mobility of the virus glycoprotein HN in SDS-PAGE, corresponding to a decrease of about 4000 in its mol. wt. We propose that the PGJ-induced alteration in the molecular structure of the HN protein prevents the insertion of this protein into the cell membrane thereby blocking virus maturation. The -unsaturated carbonyl group in the cyclopentane ring of the PGJ molecule may be necessary for antiviral activity.

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1987-04-01
2022-08-08
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References

  1. Alitalo K., Keski-OJA J., Vaheri A. 1981; Extracellular matrix proteins characterize human tumor cell lines. International Journal of Cancer 27:755–761
    [Google Scholar]
  2. Ankel H., Mittnacht S., Jacobsen H. 1985; Antiviral activity of prostaglandin A on encephalomyocarditis virus-infected cells: a unique effect unrelated to interferon. Journal of General Virology 66:2355–2364
    [Google Scholar]
  3. Benavente J., Esteban M., Jaffe B. M., Santoro M. G. 1984; Selective inhibition of viral gene expression as the mechanism of the antiviral action of PGA1 in vaccinia virus-infected cells. Journal of General Virology 65:599–608
    [Google Scholar]
  4. Crosby W. H., Forth F. W. 1956; A modification of the benzidine method for measurement of hemoglobin in plasma and urine. Blood 11:380–386
    [Google Scholar]
  5. Fukushima M., Kato T., Ota K., Aray Y., Narumiya S., Hayatshi O. 1982a; Prostaglandin D2, a potential antineoplastic agent. Biochemical and Biophysical Research Communications 105:956–964
    [Google Scholar]
  6. Fukushima M., Kato T., Ota K., Aray Y., Narumiya S., Hayaishi O. 1982b; 9-Deoxy-△9-prostaglandin D2, a prostaglandin D2 derivative with potent antineoplastic and weak smooth muscle-contracting activities. Biochemical and Biophysical Research Communications 109:626–633
    [Google Scholar]
  7. Goodwin J. S., Webb D. R. 1980; Regulation of the immune response by prostaglandins. Clinical Immunology and Immunopathology 15:106–122
    [Google Scholar]
  8. Honn K. V., Marnett L. J. 1985; Requirement of a reactive αβ-unsaturated carbonyl for inhibition of tumor growth and induction of differentiation by “A” series prostaglandins. Biochemical and Biophysical Research Communications 129:34–40
    [Google Scholar]
  9. Honn K. v., Romine M., Skoff A. 1981; Prostaglandin analogs as inhibitors of tumor cell DNA synthesis. Proceedings of the Society for Experimental Biology and Medicine 166:562–567
    [Google Scholar]
  10. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  11. Pottathil R., Chandrabose K. A., Cuatrecasas P., Lang P. J. 1980; Establishment of the interferon-mediated antiviral state: role of fatty acid cyclooxygenase. Proceedings of the National Academy of Sciences U.S. A.: 775437–5446
    [Google Scholar]
  12. Santoro M. G., Jaffe B. M. 1987; Prostaglandins and differentiation of Friend erythroleukemia cells. In Prostaglandins and Tumor Cell Proliferation and Differentiation Hammarstrom S. Edited by The Hague: Martinus Nijhoff; in press
    [Google Scholar]
  13. Santoro M. G., Benedetto A., Carruba G., Garaci E. 1980; Prostaglandin A compounds as antiviral agents. Science 209:1032–1034
    [Google Scholar]
  14. Santoro M. G., Carruba G., Garaci E., Jaffe B. M., Benedetto A. 1981; Prostaglandins of the A series inhibit Sendai virus replication in cultured cells. Journal of General Virology 53:75–83
    [Google Scholar]
  15. Santoro M. G., Jaffe B. M., Garaci E., Esteban M. 1982a; Antiviral effects of prostaglandins of the A series: inhibition of vaccinia virus replication in cultured cells. Journal of General Virology 63:435–440
    [Google Scholar]
  16. Santoro M. G., Jaffe B. M., Elia G., Benedetto A. 1982b; Prostaglandin Ai induces the synthesis of a new protein in cultured AGMK cells. Biochemical and Biophysical Research Communications 107:1179–1181
    [Google Scholar]
  17. Santoro M. G., Jaffe B. M., Esteban M. 1983; Prostaglandin A inhibits the replication of vesicular stomatitis virus: effect on virus glycoprotein. Journal of General Virology 64:2797–2801
    [Google Scholar]
  18. Santoro M. G., Crisari A., Benedetto A., Amici C. 1986; Modulation of the growth of a human erythroleukemic cell line (K562) by prostaglandins: antiproliferative action of prostaglandin A. Cancer Research 46:6073–6077
    [Google Scholar]
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