1887

Abstract

Summary

Replicative form (RF) DNA of the lymphotropic strain SL3 of Aleutian disease virus was isolated from infected cell cultures. A novel intermediate of about 7.6 kilobases was demonstrated in Hirt lysates in addition to single-stranded viral, double-stranded monomer and dimer RF DNA. The monomer RF DNA exhibited a length heterogeneity of 70 bp and 160 bp at its 3′ and 5′ termini. The two major monomer RF DNA species each contained hairpins in the extended or the foldback configurations. A central fragment between map units 0.15 and 0.88 was cloned into plasmid pUC18. The recombinant clone expressed virus-specific proteins ranging from 32000 to 74000 mol. wt.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-4-1041
1987-04-01
2019-11-17
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-4-1041
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error