1887

Abstract

SUMMARY

Incubation period and survival time were determined in C57BL mice which had been injected stereotaxically with either the 139A, ME7 or 22L strain of scrapie in one of five different brain regions (cerebral cortex, caudate nucleus, thalamus, substantia nigra, cerebellum). The injection of 139A in the caudate nucleus, thalamus, substantia nigra or cerebellum resulted in significantly shorter incubation periods than following cerebral cortex injection. For ME7, mice injected in the thalamus and cerebellum had incubation periods approximately 2 weeks shorter than the cerebral cortex group. For 22L, the incubation periods after substantia nigra or cerebellum injection were significantly shorter than after cortex injection. The cerebellum injection group had a significantly shorter incubation period than the substantia nigra injection group. The survival times for mice injected with a particular scrapie strain were directly related to the incubation periods. The groups with the shortest incubation also had the shortest survival time (e.g. 22L in the cerebellum). On histological examination, 139A and ME7 produced brain lesions in all brain areas regardless of injection site. For the 22L strain, after cerebellum injection vacuolation was limited to the cerebellum, while injection into the cerebral cortex and other forebrain regions resulted in vacuolation in all brain regions examined. Despite the difference in the distribution of vacuolation seen in cerebellum- compared to cortex-injected (22L) mice, infectivity titres were similar in the cortex, cerebellum, cerebellar cortex and medulla plus pons.

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1987-03-01
2024-11-04
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References

  1. Bruce M. E., Fraser H. 1982; Focal and asymmetrical vacuolar lesions in the brains of mice infected with certain strains of scrapie. Acta neuropathologica 58:133–140
    [Google Scholar]
  2. Carp R. I., Callahan s. M. 1986; Scrapie incubation periods and end-point titers in mouse strains differing at the H-2D locus. Intervirology in press
    [Google Scholar]
  3. Carp R. I., Callahan S. M., Sersen E. A., Moretz R. C. 1984; Preclinical changes in weight of scrapie infected mice as a function of scrapie agent-mouse strain combination. Intervirology 21:61–69
    [Google Scholar]
  4. Carp R. I., Merz P. A., Moretz R. C., Somerville R. A., Callahan S. M., Wisniewski H. M. 1985; Biological properties of scrapie: an unconventional slow virus. In Subviral Pathogen of Plants and Animals: Viroids and Prions pp 425–463 Maramorosch K., Mckelvey J. J. Edited by New York: Academic Press;
    [Google Scholar]
  5. Cole s., Kimberlin R. H. 1985; Pathogenesis of mouse scrapie: dynamics of vacuolation in brain and spinal cord after intraperitoneal infection. Neuropathology and Applied Neurobiology 11:213–227
    [Google Scholar]
  6. Dickinson A. G., Fraser H. 1979; An assessment of the genetics of scrapie in sheep and mice. In Slow Transmissible Diseases of the Nervous System 1 pp 367–375 Prusiner S. B., Hadlow W. J. Edited by New York: Academic Press;
    [Google Scholar]
  7. Dickinson A. G., Fraser H., Bruce M. E. 1978; Targeting of lesions and variation in agent concentration in different areas of the brain in scrapie. Neuropathology and Applied Neurobiology 4:241
    [Google Scholar]
  8. Dickinson A. G., Bruce M. E., Fraser H., Kimberlin R. H. 1984; Scrapie strain differences: the implications of stability and innovation. In Proceedings of Workshop on Slow Transmissible Diseases pp 105–118 Tokyo: Japanese Ministry of Health and Welfare;
    [Google Scholar]
  9. Fraser H. 1976; The pathology of natural and experimental scrapie. In Slow Virus Diseases of Animals and Man pp 267–305 Kimberlin R. H. Edited by Amsterdam & New York: North-Holland;
    [Google Scholar]
  10. Fraser H. 1979; Neuropathology of scrapie: the precision of the lesions and their diversity. In Slow Transmissible Diseases of the Nervous System 1 pp 387–406 Prusiner S. B., Hadlow W. J. Edited by New York: Academic Press;
    [Google Scholar]
  11. Fraser H. 1982; Neuronal spread of scrapie agent and targeting of lesions within the retino-tectal pathway. Nature; London: 295149–150
    [Google Scholar]
  12. Fraser H., Dickinson A. G. 1968; The sequential development of the brain lesions of scrapie in three strains of mice. Journal of Comparative Pathology 78:301–311
    [Google Scholar]
  13. Gajdusek D. c. 1977; Unconventional viruses and the origin and disappearance of kuru. Science 197:943–960
    [Google Scholar]
  14. Gorde J. M., Tamalet J., Toga M., Bert J. 1982; Changes in the nigrostriatal system following microinjection of an unconventional agent. Brain Research 240:87–93
    [Google Scholar]
  15. Hadlow W. J. 1959; Scrapie and Kuru. Lancet ii:289–290
    [Google Scholar]
  16. Hadlow W. J., Eklund C. M., Kennedy R. C., Jackson T. A., Whitford H. W. 1974; Course of experimental scrapie virus infection in the goat. Journal of Infectious Diseases 129:559–567
    [Google Scholar]
  17. Hadlow W. J., Race R. E., Kennedy R. C. 1979; Natural infection of sheep with scrapie virus. In Slow Transmissible Diseases of the Nervous System 2 pp 3–12 Prusiner S. B., Hadlow W. J. Edited by New York: Academic Press;
    [Google Scholar]
  18. Hadlow W. J., Kennedy R. C., Race R. E. 1980; Virologic and neurohistologic findings in dairy goats affected with natural scrapie. Veterinary Pathology 17:187–199
    [Google Scholar]
  19. Kimberlin R. H. 1984; Scrapie: the disease and the infectious agent. Trends in Neurosciences 7:312–316
    [Google Scholar]
  20. Kimberlin R. N., Walker C. A. 1982; Pathogenesis of mouse scrapie : patterns of agent replication in different parts of the CNS following intraperitoneal infection. Journal of the Royal Society of Medicine 75:618–624
    [Google Scholar]
  21. Kimberlin R. H., Walker C. A. 1983; Invasion of the CNS by scrapie agent and its spread to different parts of the brain. In Virus Non-conventionnels et Affections du Systéme Nerveux Central pp 15–33 Court L. A., Cathala F. Edited by Paris: Masson;
    [Google Scholar]
  22. Kimberlin R. H., Walker C. A. 1986; Pathogenesis of scrapie (strain 263K) in hamsters infected intracerebrally, intraperitoneally or intraocularly. Journal of General Virology 67:255–263
    [Google Scholar]
  23. Marsh R. F., Sipe J. E., Morse S. S. 1976; Transmissible mink encephalopathy. Reduced spongiform degeneration in aged mink of the Chediak-Higashi genotype. Laboratory Investigation 34:381–386
    [Google Scholar]
  24. Masters C. L., Gajdusek D. C., Gibbs C. J. Jr 1981; Creutzfeldt-Jakob disease virus isolations from the Gerstmann-Straussler syndrome. Brain 104:559–588
    [Google Scholar]
  25. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  26. Slotnik B. M., Leonard C. M. 1975 A Stereotaxic Atlas of the Albino Mouse Forebrain DHEW Publication No. (ADM) 75–100 Rockville; U.S Department of Health, Education and Welfare:
    [Google Scholar]
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