1887

Abstract

SUMMARY

Ultrastructural changes in cells infected with Kunjin virus were characterized from 12 to 72 h post-infection. Early in infection (16 h), there were no prominent ultrastructural changes except for an increase in the number of vacuoles in the cytoplasm. As the infection progressed the rough endoplasmic reticulum appeared to lengthen and whorls of fibres were observed within some vacuoles. Virus particles were observed in small numbers scattered in the cytoplasm between 24 to 30 h after infection. However, by 48 h, large numbers of morphologically mature virus particles were visible, usually in association with the rough endoplasmic reticulum, with the clusters of vesicles or with convoluted smooth membranes. Although no definite evidence of precursor particles or of budding of virions was obtained, the clusters of vesicles and the various membranous structures appeared to play a role in the morphogenesis of this virus. The results are compatible with the hypothesis that maturation of virus particles occurs within all these virus-induced structures.

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/content/journal/jgv/10.1099/0022-1317-68-2-577
1987-02-01
2021-10-20
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References

  1. Boulton R. W., Westaway E. G. 1976; Replication of the flavivirus Kunjin: proteins, glycoproteins and maturation associated with cell membranes. Virology 69:416–430
    [Google Scholar]
  2. Calberg-Bacq C. M., Rentier-Delrue F., Osterrieth P. M., Duchesne P. Y. 1975; Electron microscopy studies on Banzi virus particles and its development in suckling mice brains. Journal of Ultrastructure Research 53:193–203
    [Google Scholar]
  3. Cardiff R. D., Dalrymple J. M., Russell P. K. 1973; RNA polymerase in group B arbovirus (dengue-2) infected cells. Archiv für die gesamte Virusforschung 40:392–396
    [Google Scholar]
  4. Demsey A., Steere R. L., Brandt W. E., Veltri B. J. 1974; Morphology and development of dengue-2 virus employing the freeze-fracture and thin section techniques. Journal of Ultrastructure Research 46:103–116
    [Google Scholar]
  5. Filshie B. K., Rehacek J. 1968; Studies of the morphology of Murray Valley encephalitis and Japanese encephalitis viruses growing in cultured mosquito cells. Virology 34:435–443
    [Google Scholar]
  6. Leary K., Blair C. D. 1980; Sequential events in the morphogenesis of Japanese encephalitis virus. Journal of Ultrastructure Research 72:123–129
    [Google Scholar]
  7. Murphy F. A., Harrison A. K., Gary G. W.Jr Whitfield S. M, Forrester F. G. 1968; St. Louis encephalitis virus infection of mice. Electron microscopic studies of central nervous system. Laboratory Investigation 19:652–662
    [Google Scholar]
  8. Ng M. L., Westaway E. G. 1980; Establishment of persistent infections by flaviviruses in Aedes albopictus cells. In Invertebrate Systems in vitro pp. 389–402 Kurstak E., Maramorosch K., Dubendorfer X. Edited by New York: Academic Press;
    [Google Scholar]
  9. Palade G. E. 1952; A study of fixation for electron microscopy. Journal of Experimental Medicine 95:285–298
    [Google Scholar]
  10. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–212
    [Google Scholar]
  11. Sabatini D. D., Bensch K., Barnett R. J. 1963; Cytochemistry and electron microscopy-presentation of cellular ultrastructure and enzymatic activity by aldehyde fixation. Journal of Cell Biology 17:19–58
    [Google Scholar]
  12. Sriurairatna S., Bhamarapravati N., Phelavadhtana O. 1973; Dengue virus infection of mice: morphology and morphogenesis of dengue type-2 virus in suckling mouse neurones. Infection and Immunity 8:1017–1028
    [Google Scholar]
  13. Westaway E. G. 1973; Proteins specified by Group B togaviruses in mammalian cells during productive infections. Virology 51:454–465
    [Google Scholar]
  14. Westaway E. G., Ng M. L. 1980; Replication of flaviviruses: separation of membrane translation sites of Kunjin virus proteins and of cell proteins. Virology 106:107–122
    [Google Scholar]
  15. Zebovitz E., Leong J. K. L., Doughty S. C. 1974; Involvement of the host cell nuclear envelope membranes in the replication of Japanese encephalitis virus. Infection and Immunity 10:204–211
    [Google Scholar]
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