1887

Abstract

SUMMARY

Biological activities of antisera against synthetic oligopeptides were examined. The peptide antisera were directed against amino acids 6 to 12 (pep1), 124 to 131 (pep2), 256 to 262 (pep3), 283 to 290 (pep4) and 434 to 441 (pep5) of the viral envelope glycoprotein (gp70). Peptide-specific antisera did not neutralize viral infectivity. However, antibodies to pep4 and pep5, which bound to the hydrophobic part of gp70, mediated the complement-dependent lysis of Friend murine leukaemia virus (FLV)-infected cells. STU mice were immunized against FLV-induced erythroleukaemia with synthetic oligopeptides. Vaccines containing only one of these peptides (single-peptide vaccines) as well as multi-peptide vaccines containing pep1, −4, −5 or pep1, −2, −3, −4, −5 were used. Whereas the immunizations with single-peptide vaccines did not protect the immunized mice from FLV-induced erythroleukaemia, multi-peptide vaccines enhanced the survival rate and incubation period after FLV challenge. These results revealed that immunological reactions distinct from neutralizing antibodies can be evoked by immunization with synthetic peptides and can confer limited protection against FLV-induced erythroleukaemia.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-2-515
1987-02-01
2024-05-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/2/JV0680020515.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-2-515&mimeType=html&fmt=ahah

References

  1. Anderer F. A., Schlumberger H. D. 1965; Properties of different artificial antigens immunologically related to tobacco mosaic virus. Biochimica et biophysica acta 97:503–509
    [Google Scholar]
  2. Bayer H., Gruber W., Schneider J., Hunsmann G. 1984; Structural and immunological characterization of Friend murine leukaemia virus glycopolypeptide using synthetic oligopeptides. EMBO Journal 3:1925–1930
    [Google Scholar]
  3. Bittle J. L., Houghten R. A., Alexander H., Shinnick T. M., Sutcliffe J. G., Lerner R. A. 1982; Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature; London: 29830–33
    [Google Scholar]
  4. Chesebro B., Wehrly K., Cloyd M., Britt W., Portis J., Collins J., Nishio J. 1981; Characterization of mouse monoclonal antibodies specific for Friend murine leukemia virus-induced erythroleukemia cells: Friend-specific and FMR-specific antigens. Virology 112:131–144
    [Google Scholar]
  5. Emini E., Bradford A. J., Winkler E. 1983; Priming for and induction of anti-poliovirus neutralizing antibodies by synthetic peptides. Nature; London: 304699–703
    [Google Scholar]
  6. Francis M. J., Fry C. M., Rowlands D. J., Brown F., Bittle J. L., Houghten R. A., Lerner R. A. 1985; Immunological priming with synthetic peptides of foot-and-mouth disease virus. Journal of General Virology 66:2347–2354
    [Google Scholar]
  7. Friend C. 1957; Cell-free transmission in adult Swiss mice of a disease having the character of a leukemia. Journal of Experimental Medicine 105:307–318
    [Google Scholar]
  8. Göbel W. F. 1939; Studies on antibacterial immunity induced by artificial antigens. I. Immunity to experimental pneumococcal infection with an antigen containing cellobiuronic acid. Journal of Experimental Medicine 69:353–364
    [Google Scholar]
  9. Hunsmann G. 1985; Subunit vaccines against exogenous retroviruses: overview and perspectives. Cancer Research (Suppl) 45:4691s–4693s
    [Google Scholar]
  10. Hunsmann G., Moennig V., Schäfer W. 1975; Properties of mouse leukemia viruses. IX. Active and passive immunization of mice against Friend leukemia with isolated viral gp71 glycoprotein and its corresponding antiserum. Virology 66:327–329
    [Google Scholar]
  11. Hunsmann G., Claviez M., Mönnig V., Schwarz H., Schäfer W. 1976; Properties of mouse leukemia viruses. X. Occurrence of viral structural antigens on the cell surface as revealed by a cytotoxicity test. Virology 69:157–169
    [Google Scholar]
  12. Hunsmann G., Schneider J., Schulz A. 1981; Immunoprevention of Friend virus-induced erythroleukemia by vaccination with viral envelope glycoprotein complexes. Virology 113:602–612
    [Google Scholar]
  13. Koch W., Hunsmann G., Friedrich R. 1983; Nucleotide sequence of the envelope gene of Friend murine leukemia virus. Virology 45:1–9
    [Google Scholar]
  14. Lenz J., Crowther R., Straceski A., Haseltine W. 1982; Nucleotide sequence of AKV env gene. Journal of Virology 42:519–529
    [Google Scholar]
  15. Liu W. T., Engelman R. w., Trang L. Q., Hau K., Good R. A., Day N. K. 1984; Appearance of cytotoxic antibody to viral gp70 on feline lymphoma cells (FL-74) in cats during ex vivo immunoadsorption therapy: quantitation, characterization, and association with remission of disease and disappearance of viremia. Proceedings of the National Academy of Sciences, U.S.A 81:3516–3520
    [Google Scholar]
  16. Oroszlan S., Nowinski R. C. 1980; Lysis of retroviruses with monoclonal antibodies against viral envelope proteins. Virology 101:296–299
    [Google Scholar]
  17. Rowe W. P., Pugh W. E., Hartley J. W. 1970; Plaque assay techniques for murine leukemia viruses. Virology 42:1136–1139
    [Google Scholar]
  18. Schäfer W. 1979; The inbred mouse strain STU. Development and properties. Zeitschrift für Naturforschung 34C:306–309
    [Google Scholar]
  19. Schäfer W., Schwarz H., Thiel H. J., Wecker E., Bolognesi D. P. 1976; Properties of mouse leukemia viruses. XIII. Serum therapy of virus-induced murine leukemias. Virology 75:401–418
    [Google Scholar]
  20. Schneider J., Falk H., Hunsmann G. 1980; Envelope polypeptides of Friend leukemia virus: purification and structural analysis. Journal of Virology 33:597–605
    [Google Scholar]
  21. Seifert E., Claviez M., Frank H., Hunsmann G., Schwarz H., Schäfer W. 1975; Properties of mouse leukemia viruses. XII. Produktion grösserer Mengen von Friendvirus durch eine permanente Zellsuspen-sionskultur (Eveline-Suspensions-Zellen). Zeitschrift für Naturforschung 30C:689–700
    [Google Scholar]
  22. Sun D., Lohmann-Matthes M.-L. 1982; Functionally different subpopulations of mouse macrophages recognized by monoclonal antibodies. European Journal of Immunology 12:134–140
    [Google Scholar]
  23. Sutcliffe J. G., Shinnick T. M., Green N., Lerner R. A. 1983; Antibodies that react with predetermined sites on proteins. Science 219:660–666
    [Google Scholar]
  24. Tainer J. A., Getzoff E. D., Alexander H., Houghten R. A., Olsen A. J., Lerner R. A. 1984; The reactivity of antipeptide antibodies is a function of the atomic mobility of sites in a protein. Nature; London: 312127–134
    [Google Scholar]
  25. Walter G., Doolittle R. F. 1983; Antibodies against synthetic peptides. In Genetic Engineering: Principles and Methods 5 pp. 61–91 Holländer A., Setlow J. K. Edited by New York: Plenum Press;
    [Google Scholar]
  26. Wolff L., Scolnick E., Ruscetti S. 1983; Envelope gene of the Friend spleen focus-forming virus: deletion and insertions in the 3′ gp70/p15E encoding region have resulted in unique features in the primary structure of its protein product. Proceedings of the National Academy of Sciences, U.S.A 80:4718–4722
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-2-515
Loading
/content/journal/jgv/10.1099/0022-1317-68-2-515
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error