1887

Abstract

SUMMARY

DNA of human papillomavirus (HPV) type 45, a new HPV type 18-related papillomavirus of the genital tract, was cloned from a recurrent cervical lesion displaying mild to moderate dysplasia with koilocytosis. HPV-45 DNA was identified in paraffin sections of biopsies of both the initial and recurrent lesions of the patient, taken 7 months apart. HPV-45 DNA hybridized efficiently to that of many different HPV types under low and moderate stringency conditions (T − 37 °C to T − 25 °C) but with only HPV-18 DNA under high stringency conditions (T − 17 °C). HPV-45 DNA was distinguished from HPV-18 DNA by (i) differences in restriction enzyme digest patterns, (ii) lack of hybridization at T - 17 °C between HPV-18 and some fragments of HPV-45, (iii) a value of 25 % in liquid reassociation kinetics between HPV-18 and HPV-45 and (iv) differences in intensities of hybridization with selected tissue DNAs. The prevalence of HPV-45 infection in the genital tract was low. In tests of over 600 tissue DNAs from female genital tract lesions, HPV-45 sequences were detected in three additional tissues, one each of invasive cervical carcinoma, condyloma, and normal cervical epithelium. HPV-45 is a newly recognized papillomavirus which rarely infects the genital tract and is associated with lesions across a wide histological spectrum.

Keyword(s): genital tract , human and papillomavirus
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-12-3073
1987-12-01
2024-04-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/12/JV0680123073.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-12-3073&mimeType=html&fmt=ahah

References

  1. Bergeron C., Ferenczy A., Shah K.v., Naghashfar Z. 1987; Multicentric human papillomavirus infections of the female genital tract: correlation of viral types with abnormal mitotic figures, colposcopic presentation and location. Obstetrics and Gynecology 69:736–742
    [Google Scholar]
  2. Boshart M., Gissmann L., Ikenberg A., Kleinheinz W., Scheurlen W., Zur Hausen H. 1984; A new type of papillomavirus DNA, its presence in genital cancer biopsies and in cell lines derived from cervical cancer. EMBO Journal 3:1151–1157
    [Google Scholar]
  3. Denhardt D. T. 1966; A membrane filter technique for the detection of complementary DNA. Biochemical and Biophysical Research Communications 23:641–646
    [Google Scholar]
  4. De Villiers E. M., Schneider A., Gross G., Zur Hausen H. 1986; Analysis of benign and malignant urogenital tumors for human papillomavirus infection by labeling cellular DNA. Medical Microbiology and Immunology 174:281–286
    [Google Scholar]
  5. Dürst M., Kleinheinz A., Hotz M., Gissmann L. 1985; The physical state of human papillomavirus type 16 DNA in benign and malignant genital tumours. Journal of General Virology 66:1515–1522
    [Google Scholar]
  6. Gissmann L., Schneider A. 1986; Human papillomavirus DNA in preneoplastic and neoplastic genital lesions. In Banbury Report 21 pp 217–224 Peto R., Zur Hausen H. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  7. Gissmann L., Diehl V., Schultz-Coulon H. J., Zur Hausen H. 1982; Molecular cloning and characterization of human papillomavirus DNA derived from a laryngeal papilloma. Journal of Virology 44:393–400
    [Google Scholar]
  8. Gupta J. W., Gupta P. K., Shah K. V., Kelly D. P. 1983; Distribution of human papillomavirus antigen in cervicovaginal smears and cervical tissues. International Journal of Gynecologic Pathology 2:160–170
    [Google Scholar]
  9. Gupta J., Gendelman H. E., Naghashfar Z., Gupta P., Rosenshein N., Sawada E., Woodruff J. D., Shah K. V. 1985; Specific identification of human papillomavirus type in cervical smears and paraffin sections by in situhybridization with radioactive probes: a preliminary communication. International Journal of Gynecologic Pathology 4:211–218
    [Google Scholar]
  10. Loenen W. A. M., Brammar W. I. 1980; Bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes. Gene 20:249–259
    [Google Scholar]
  11. Lorincz A. T., Temple G. F., Patterson J. A., Jenson A. B., Kurman R. J., Lancaster W. D. 1986; Correlation of cellular atypia and human papillomavirus deoxyribonucleic acid sequences in exfoliated cells of the uterine cervix. Obstetrics and Gynecology 64:508–512
    [Google Scholar]
  12. McConaughy B. L., Laurd C. D., Mccarthy B. J. 1969; Nucleic acid reassociation in formamide. Biochemistry 8:3289–3295
    [Google Scholar]
  13. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A Laboratory Manual New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  14. Melton D. A., Kreig P. A., Rebgliati M. R., Maniatis T., Zinn K., Green M. R. 1984; Efficient in vitrosynthesis of biologically active RNA and RNA hybridization probe from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Research 12:7035–7056
    [Google Scholar]
  15. Naghashfar Z., Sawada E., Kutcher M. J., Swancar J., Gupta J., Daniel R., Kashima H., Woodruff J. D., Shah K. 1985; Identification of genital tract papillomaviruses HPV-6 and HPV-16 in warts of the oral cavity. Journal of Medical Virology 17:313–324
    [Google Scholar]
  16. Orth G., Breitburd F., Favre M., Croissant O. 1977; Papilloma viruses: possible role in human cancer. In Origins of Human Cancer, Cold Spring Harbor Conferences on Cell Proliferation, 4 pp 1043–1068 Hiatt H. H., Watson J. D., Winsten J. A. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  17. Reid R., Greenberg M., Jenson B., Husain M., Willett J., Daoud Y., Temple G., Stanhope R., Sherman A., Phibbs G., Lorincz A. 1987; Sexually transmitted papillomaviral infections, the anatomic distribution and pathologic grade of neoplastic lesions associated with different types. Americal Journal of Obstetrics and Gynecology 156:212–222
    [Google Scholar]
  18. Schwarz E., Durst M., Demankowski C., Lattermann O., Zech R., Wolfsperger E., Suhai S., Zur Hausen H. 1983; DNA sequence and organization of genital human papillomavirus type 6b. EMBO Journal 2:2341–2348
    [Google Scholar]
  19. Schwarz E., Freese V., Gissmann L., Mayer W., Roggenbuck B, Zur Hausen H. 1985; Structure and transcription of human papillomavirus sequences in cervical carcinoma cells. Nature, London 314:111–114
    [Google Scholar]
  20. Wetmur J. G., Davidson N. 1968; Kinetics of renaturation of DNA. Journal of Molecular Biology 31:349–370
    [Google Scholar]
  21. Zur Hausen N. 1980; Papilloma viruses. In DNA Tumor Viruses pp. 371–382 Tooze J. Edited by New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-12-3073
Loading
/content/journal/jgv/10.1099/0022-1317-68-12-3073
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error