1887

Abstract

Summary

To determine the extent and nature of the antigenic variation of four U.S.A. serotypes of bluetongue virus (BTV), the complete nucleotide sequence was determined for cDNA clones representing the L2 dsRNA of BTV serotype 13, the gene that codes for the outer capsid neutralization antigen (VP2). The predicted amino acid sequence of the protein was compared with the VP2 sequences of the U.S.A. serotypes of BTV-10, BTV-11 and BTV-17. Diagon comparisons, hydropathic plots and analyses of potential secondary structure of the four proteins indicated that all four VP2 proteins were structurally similar. However, the VP2 protein of BTV-13 was found to exhibit only 40% homology with the VP2 species of the other three viruses. The comparative sequence data indicated that there were regions of the proteins with greater variability than other regions, as expected for proteins that vary antigenically but are structurally similar.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-11-2967
1987-11-01
2024-04-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/11/JV0680112967.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-11-2967&mimeType=html&fmt=ahah

References

  1. Ghiasi H., Purdy M. A., Roy P. 1985; The complete sequence of bluetongue virus serotype 10 segment 3 and its predicted VP3 polypeptide compared with those of BTV serotype 17. Virus Research 3:181–190
    [Google Scholar]
  2. Ghiasi H., Fukusho A., Eshita Y., Roy P. 1987; Identification and characterisation of conserved and variable regions in the neutralization VP2 gene of bluetongue virus. Virology in press
    [Google Scholar]
  3. Huismans H., Eramus B. J. 1981; Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort Journal of Veterinary Research 48:51–58
    [Google Scholar]
  4. Huismans H., Van Der Walt N. T., Cloete M., Erasmus B. J. 1983; The biochemical and immunological characterisation of bluetongue virus outer capsid protein polypeptides. In Double-stranded RNA Viruses pp. 165–172 Compans R. W., Bishop D. H. L. Edited by New York: Elsevier;
    [Google Scholar]
  5. Kahlon J., Sugiyama K., Roy P. 1983; Molecular basis of bluetongue virus neutralization. Journal of Virology 48:627–632
    [Google Scholar]
  6. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  7. Lee J., Roy P. 1986; Nucleotide sequence of a cDNA clone of RN A segment 10 of bluetongue virus (serotype 10). Journal of General Virology 67:2833–2837
    [Google Scholar]
  8. Maxam A. M., Gilbert W. 1980; Sequencing end-labelled DNA with base-specific chemical cleavages. Methods in Enzymology 65:499–560
    [Google Scholar]
  9. Purdy M. A., Petre J., Roy P. 1984; Cloning of the bluetongue virus L3 gene. Journal of Virology 51:754–759
    [Google Scholar]
  10. Purdy M. A., Ghiasi H., Rao C. D., Roy P. 1985; Complete sequence of bluetongue virus L2 RN A that codes for the antigen recognized by neutralizing antibodies. Journal of Virology 55:826–830
    [Google Scholar]
  11. Purdy M. A., Ritter G. D., Roy P. 1986; Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10. Journal of General Virology 67:957–962
    [Google Scholar]
  12. Rao C. D., Sugiyama K., Rao P. 1983a; Evolution of bluetongue virus serotype 17. American Journal of Tropical Medicine and Hygiene 32:865–870
    [Google Scholar]
  13. Rao C. D., Kiuchi A., Roy P. 1983b; Homologous terminal sequences of the genome double-stranded RNAs of bluetongue viruses. Journal of Virology 46:378–383
    [Google Scholar]
  14. Roy P., Sugiyama K., Rao C. D., Kusari J., Purdy M., Collison E. 1985; Molecular epidemiology of two U.S. Orbiviruses: bluetongue virus and epizootic hemorrhagic disease virus. In Proceedings of International Symposium on Bluetongue and Related Orbiviruses pp. 589–595 Lynwood Barber T. L., Jochim M. M. Edited by New York: Alan R. Liss;
    [Google Scholar]
  15. Staden R. 1982; An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences. Nucleic Acids Research 10:2951–2961
    [Google Scholar]
  16. Sugiyama K., Bishop D. H. L., Roy P. 1981; Analyses of the genomes of bluetongue viruses recovered in the United States. I. Oligonucleotide fingerprint studies that indicate the existence of naturally occurring reassortant BTV isolates. Virology 114:210–217
    [Google Scholar]
  17. Sugiyama K., Bishop D. H. L., Roy P. 1982; Analyses of the genomes of bluetongue virus isolates recovered from different states of the United States and at different times. American Journal of Epidemiology 115:332–347
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-11-2967
Loading
/content/journal/jgv/10.1099/0022-1317-68-11-2967
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error