1887

Abstract

SUMMARY

Antiserum raised against purified potato yellow dwarf virus (PYDV) reacted specifically with all five viral structural proteins in immunoblots and also with ultrathin sections of infected tobacco cells. In these sections viral proteins were detected with gold-labelled anti-IgG antibodies and gold particles were predominantly located over the nucleoplasm and over PYDV virus particles either at the periphery of nuclei or in the cytoplasm. Control sections of uninfected or healthy cells were not labelled. The results suggest that the nucleoplasm is the only site of accumulation of PYDV structural proteins.

Keyword(s): immunogold labelling , nucleus and PYDV
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-10-2723
1987-10-01
2024-12-04
Loading full text...

Full text loading...

/deliver/fulltext/jgv/68/10/JV0680102723.html?itemId=/content/journal/jgv/10.1099/0022-1317-68-10-2723&mimeType=html&fmt=ahah

References

  1. Adam G., Hsu L. N. 1984; Comparison of structural proteins from two potato yellow dwarf viruses. Journal of General Virology 65:991–994
    [Google Scholar]
  2. Black L. N. 1970; Potato yellow dwarf virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses35
    [Google Scholar]
  3. Black L. M., Smith K. M., Markham R. 1965; Ultrastructure of potato yellow dwarf virus. Virology 27:446–449
    [Google Scholar]
  4. Clowes F. A. L., Juniper B. E. 1968 Plant Cells, Botanical Monographs 8 Oxford: Blackwell;
    [Google Scholar]
  5. Falk B. W., Weathers L. G. 1983; Comparison of potato yellow dwarf virus serotypes. Phytopathology 73:8185
    [Google Scholar]
  6. Francki R. I. B. 1973; Plant rhabdoviruses. Advances in Virus Research 18:257–345
    [Google Scholar]
  7. Francki R. I. B., Kitajima E. W., Peters D. 1981; Rhabdoviruses. In Handbook of Plant Virus Infections and Comparative Diagnosis pp 455–489 Kurstak E. M. Edited by Amsterdam: Elsevier;
    [Google Scholar]
  8. Francki R. I. B., Milne R. G., Hatta H. 1985; Plant rhabdoviridae. In Atlas of Plant Viruses 1: pp 73–100 Boca Raton: CRC Press;
    [Google Scholar]
  9. Horisberger M. 1979; Evaluation of colloidal gold as a cytochemical marker for transmission and scanning electron microscopy. Biologie Cellulaire 36:253–258
    [Google Scholar]
  10. Jackson A. O., Christie S. R. 1977; Purification and some physicochemical properties of sonchus yellow net virus. Virology 77:344–355
    [Google Scholar]
  11. Knudson D. L. 1972; The proteins of potato yellow dwarf virus. Virology 17:285–295
    [Google Scholar]
  12. Kyhse-Andersen J. 1984; Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. Journal of Biochemical and Biophysical Methods 10:203–209
    [Google Scholar]
  13. Laemmli U. k. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 221680–685
    [Google Scholar]
  14. Lane L. C. 1978; A simple method for stabilizing protein-sulfhydryl groups during SDS-gel electrophoresis. Analytical Biochemistry 86:655–664
    [Google Scholar]
  15. Langenberg W. G. 1979; Chilling of tissue prior to glutaraldehyde fixation preserves fragile inclusions of several plant viruses. Journal of Ultrastructure Research 66:120–131
    [Google Scholar]
  16. Lin N. S., Langenberg W. 1983; Immunohistochemical localization of barley stripe mosaic virions in infected wheat cells. Journal of Ultrastructure Research 84:16–23
    [Google Scholar]
  17. Martelli G. P., Russo M. 1977; Rhabdoviruses of plants. In The Atlas of Insect and Plant Viruses pp 181–213 Maramorosch K. Edited by New York: Academic Press;
    [Google Scholar]
  18. Moeremans M., Daneels G., Demay J. 1985; Sensitive colloidal metal (gold or silver) staining of protein blots on nitrocellulose membranes. Analytical Biochemistry 145:315–321
    [Google Scholar]
  19. Peters D. 1981; Plant rhabdovirus group.. Commonwealth Mycological Institute /Association of Applied Biologists Descriptions of Plant Viruses244
    [Google Scholar]
  20. Reynolds E. S. 1963; The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. Journal of Cell Biology 17:208–213
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-68-10-2723
Loading
/content/journal/jgv/10.1099/0022-1317-68-10-2723
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error