1887

Abstract

Summary

Previous work has shown that a novel protein kinase is induced after infection of cultured cells with herpes simplex virus type 1 (HSV-1). Separately, it has been reported that the protein encoded by HSV-1 gene US3 shows similarity in its amino acid sequence to members of the protein kinase family of eukaryotes. We have investigated the possibility that these two observations are connected by preparing an antiserum to a synthetic oligopeptide corresponding to the carboxy-terminal eight amino acids of the US3 protein. This antiserum reacted on immunoblots with a polypeptide of apparent molecular weight 68000 from extracts of cells which had been infected with HSV-1. The antiserum also reacted strongly with a 68000 molecular weight species from a preparation of the novel HSV-1 protein kinase which had been extensively purified and resolved from other protein kinases. In addition, the purified preparation phosphory-lated a protein species, also of 68000 apparent molecular weight, when incubated with [γ-P]ATP. These data are consistent with gene US3 encoding the novel protein kinase induced after infection of cells with HSV-1.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-10-2699
1987-10-01
2019-11-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-10-2699
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error