1887

Abstract

SUMMARY

Synthetic peptides corresponding to six predicted immunogenic sites on human rhinovirus type 2 (HRV2) have been tested for their reactivity with an anti-virion antibody and for their ability to elicit neutralizing antibody. Four of the peptides reacted with HRV2 antiserum in an indirect ELISA. Rabbit antisera produced to three of these four peptides, one each from VP1, VP2 and VP3, reacted with the virus in an indirect ELISA and with the corresponding proteins by Western blotting. Furthermore, antiserum to one of the peptides, designed to cover the neutralization epitope NIm-II on VP2, not only reacted well in a sandwich ELISA and in an immunoprecipitation test but also neutralized virus infectivity.

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/content/journal/jgv/10.1099/0022-1317-68-10-2687
1987-10-01
2022-01-28
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References

  1. Callahan P. L., Mizutani S., Colonno R. J. 1985; Molecular cloning and complete sequence determination of RNA genome of human rhinovirus type 14. Proceedings of the National Academy of Sciences U.S.A.: 82732–736
    [Google Scholar]
  2. Francis M. J., Fry C. M., Rowlands D. J., Bittle J. L., Houghten R. A., Lerner R. A., Brown F. 1987; Immune response to uncoupled peptides of foot-and-mouth disease virus. Immunology 60:1–6
    [Google Scholar]
  3. Houghten R. A. 1985; General method for the rapid synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids. Proceedings of the National Academy of Sciences U.S.A.: 825131–5135
    [Google Scholar]
  4. Liu F., Zinnecker M., Hamaoka T., Katz D. H. 1979; New procedures for preparation and isolation of conjugates of proteins and a synthetic copolymer of D-amino acids and immunochemical characterisation of such conjugates. Biochemistry 18:690–697
    [Google Scholar]
  5. Mccullough K. C., Crowther J. R., Butcher R. N. 1985; Alteration in antibody reactivity with foot-and- mouth disease virus (FMDV) 146S antigen before and after binding to a solid phase or complexing with specific antibody. Journal of Immunological Methods 82:91–100
    [Google Scholar]
  6. Medappa K. C., Mclean C., Rueckert R. R. 1971; On the structure of rhinovirus 1A. Virology 44:259–270
    [Google Scholar]
  7. Merrifield R. B. 1963; Solid phase peptide synthesis. I. The synthesis of a tetrapeptide. Journal of the American Chemical Society 85:2149–2154
    [Google Scholar]
  8. Rossmann M. G., Arnold E., Erickson J. W., Frankenberger E. A., Griffith J. P., Hecht H-J., Johnson E., Kramer G., Luo M., Mosser A. G., Rueckert R. R., Sherry B., Vriend G. 1985; Structure of a human common cold virus and functional relationship to other picornaviruses. Nature; London: 317145–153
    [Google Scholar]
  9. Rueckert R. R. 1976; On the structure and morphogenesis of picornaviruses. In Comprehensive Virology 6 pp 131–213 Fraenkel-Conrat H., Wagner R. R. Edited by New York: Plenum Press;
    [Google Scholar]
  10. Sherry B., Rueckert R. R. 1985; Evidence for at least two dominant neutralization antigens on human rhinovirus. Journal of Virology 53:137–143
    [Google Scholar]
  11. Sherry B., Mosser A. G., Colonno R. J., Rueckert R. R. 1986; Use of monoclonal antibodies to identify four neutralization immunogens on a common cold picomavirus human rhinovirus 14. Journal of Virology 51:246–257
    [Google Scholar]
  12. Skern T., Sommergruber W., Blaas D., Gruendler P., Fraundorfer F., Pieler CH., Fogy I., Kuechler E. 1985; Human rhinovirus 2: complete nucleotide sequence and proteolytic processing signals in the capsid protein region. Nucleic Acids Research 13:2111–2126
    [Google Scholar]
  13. Skern T., Neubauer C., Frasel L., Gründler P., Sommergruber W., Zorn M., Kuechler E., Blaas D. 1987; A neutralizing epitope on human rhinovirus type 2 includes amino acid residues between 153 and 164 of virus capsid protein VP2. Journal of General Virology 68:315–323
    [Google Scholar]
  14. Stanway G., Hughes P. J., Mountford R. C., Minor P. D., Almond J. W. 1984; The complete nucleotide sequence of a common cold virus: human rhinovirus 14. Nucleic Acids Research 12:7859–7875
    [Google Scholar]
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