1887

Abstract

Summary

Murine T cell clones specific for the Kunjin (KUN), West Nile (WN) and Murray Valley encephalitis (MVE) flaviviruses were generated following priming . Clones were isolated by limiting dilution and maintained in culture with antigen stimulation and interleukin-2 (IL-2). The cells were characterized as having the Thy 1, L3T4 and Lyt 2 phenotype by immunofluorescence. All clones proliferated strongly and secreted high levels of IL-2 and IL-3 in response to homologous antigen. Both KUN- and WN-specific clones showed extensive cross-reactivity to KUN and WN antigen, but recognized MVE to a lesser extent. In contrast, MVE-specific clones cross-reacted strongly with both KUN and WN. These data show that antigen-specific, major histocompatibility complex-restricted L3T4 T cells are generated during flavivirus infection and are cross-reactive for viruses of the same subgroup.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-68-10-2655
1987-10-01
2019-10-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-68-10-2655
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error