1887

Abstract

SUMMARY

Murine T cell clones specific for the Kunjin (KUN), West Nile (WN) and Murray Valley encephalitis (MVE) flaviviruses were generated following priming . Clones were isolated by limiting dilution and maintained in culture with antigen stimulation and interleukin-2 (IL-2). The cells were characterized as having the Thy 1, L3T4 and Lyt 2 phenotype by immunofluorescence. All clones proliferated strongly and secreted high levels of IL-2 and IL-3 in response to homologous antigen. Both KUN- and WN-specific clones showed extensive cross-reactivity to KUN and WN antigen, but recognized MVE to a lesser extent. In contrast, MVE-specific clones cross-reacted strongly with both KUN and WN. These data show that antigen-specific, major histocompatibility complex-restricted L3T4 T cells are generated during flavivirus infection and are cross-reactive for viruses of the same subgroup.

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1987-10-01
2021-10-19
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References

  1. Allan J. E., Doherty P. C. 1986; Stimulation of helper/delayed-type hypersensitivity T cells by flavivirus infection: determination by macrophage procoagulant assay. Journal of General Virology 67:39–46
    [Google Scholar]
  2. Chen C., Schwartz R. H. 1982; Preparation of TCGF from a T-cell tumour (EL4). In Isolation, Characterization and Utilization of T Lymphocyte Clones p. 449 Fatham C. G., Fitch F. W. Edited by New York: Academic Press;
    [Google Scholar]
  3. Davidson W. F., Parish C. R. 1975; A procedure for removing red cells and dead cells from lymphoid cell suspensions. Journal of Immunological Methods 7:291–300
    [Google Scholar]
  4. Dialynas D. P., Wilde D. B., Marrack P., Pierres A., Wall K. A., Havran W., Otten G., Loken M. R., Pierres M., Kappler J., Fitch F. W. 1983; Characterization of the murine antigenic determinant designated L3T4a recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunological Reviews 14:29–56
    [Google Scholar]
  5. Farrer J. J., Fuller-Farrar J., Simon P. L., Hilfiker M. L., Stadler G. M., Farrer W. L. 1980; Thymoma production of T cell growth factor (interleukin 2). Journal of Immunology 125:2555–2558
    [Google Scholar]
  6. Gillis S., Ferm M. M., Ou W., Smith K. A. 1978; T cell growth factor: parameters of production and a quantitative microassay for activity. Journal of Immunology 120:2027–2032
    [Google Scholar]
  7. Greenburger J. S., Eckner R. J., Sakakeeny M., Marks P., Reid D., Nabel G., Hapel A. J., Ihle J. N., Humphries K. C. 1983; Interleukin 3-dependent haematopoietic progenitor cell lines. Federation Proceedings 42:2762–2771
    [Google Scholar]
  8. Halstead S. B. 1982; Immune enhancement of viral infection. Progress in Allergy 31:301–364
    [Google Scholar]
  9. Heeg K., Reiman J., Kabelitz D., Hardt C., Wagner H. 1985; A rapid colorimetric assay for the determination of IL-2 producing helper T frequencies. Journal of Immunological Methods 77:237–246
    [Google Scholar]
  10. Henchal E. A., Mccown J. M., Burke D. S., Seguin M. C., Brandt W. E. 1985; Epitopic analysis of antigenic determinants on the surface of dengue-2 virions using monoclonal antibodies. American Journal of Tropical Medicine and Hygiene 34:162–169
    [Google Scholar]
  11. Henderson B. E., Cheshire P. P., Kirya G. B., Lule M. 1970; Immunological studies with yellow fever and selected African group B arboviruses in rhesus and vervet monkeys. American Journal of Tropical Medicine and Hygiene 19:110–118
    [Google Scholar]
  12. Kayser M., Klein H., Paasch L, Blenck H., Heeg K. 1985; Human antibody response to immunization with 17D yellow fever and inactivated TBE vaccine. Journal of Medical Virology 17:35–45
    [Google Scholar]
  13. Lamb J. R., Eckels D. D., Lake P., Johnson A. H., Hartzman R. J., Woody J. n. 1982; Antigen specific human T lymphocyte clones, induction, antigen specificity and MHC restriction of influenza virus-immune clones. Journal of Immunology 128:233–238
    [Google Scholar]
  14. Madrid DE, Porterfield J. S. 1974; The flaviviruses (group B arboviruses): a cross-neutralization study. Journal of General Virology 23:91–96
    [Google Scholar]
  15. Melchers F., Andersson J. 1986; Factors controlling the B cell cycle. Annual Review of Immunology 4:13–36
    [Google Scholar]
  16. Miller R. A., Stutman O. 1982; Enumeration of IL-2-secreting helper T cells by limiting dilution analysis, and demonstration of unexpectedly high levels of IL2 production per responding cell. Journal of Immunology 128:2258–2264
    [Google Scholar]
  17. Mosmann T. 1983; Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods 65:55–63
    [Google Scholar]
  18. Mosmann T. R., Cherwinski H., Bond M. W., Giedlin M. A., Coffman R. L. 1986; Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. Journal of Immunology 136:2348–2357
    [Google Scholar]
  19. Pang T., Wong P. Y., Pathmanathan R. 1982; Induction and characterisation of delayed-type hypersensitivity to Dengue virus in mice. Journal of Infectious Disease 146:235–242
    [Google Scholar]
  20. Price W. H., Parks J., Ganaway L, O’Leary W. 1963; A sequential immunization procedure against certain group B arboviruses. American Journal of Tropical Medicine and Hygiene 12:624–638
    [Google Scholar]
  21. Roehrig J. T., Mathews J. H., Trent D. W. 1983; Identification of epitopes on the E glycoprotein of St Louis encephalitis virus using monoclonal antibodies. Virology 128:118–126
    [Google Scholar]
  22. Sarmiento M., Glasebrook A. L., Fitch F. W. 1980; IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt2 antigens block T-cell mediated cytolysis in the absence of complement. Journal of Immunology 125:2665–2672
    [Google Scholar]
  23. Schlesinger J. J., Walsh E. E., Brandriss M. W. 1984; Analysis of 17D yellow fever virus envelope protein epitopes using monoclonal antibodies. Journal of General Virology 65:1637–1644
    [Google Scholar]
  24. Schereier M. H., Tees R. 1981; Long-term culture and cloning of specific helper T cells. Immunological Methods 11:263–275
    [Google Scholar]
  25. Schrader J. W. 1986; The panspecific hemopoietin of activated T lymphocytes (interleukin 3). Annual Review of Immunology 4:205–230
    [Google Scholar]
  26. Smith K. A. 1984; Interleukin 2. Annual Review of Immunology 2:319–334
    [Google Scholar]
  27. Westaway E. G. 1965; The neutralization of arboviruses. II. Neutralization in heterologous virus-serum mixtures with four group B arboviruses. Virology 26:528–537
    [Google Scholar]
  28. Westaway E. G., Brinton M. A., Gaidamovich S. y., Horzinek M. C., Igarashi A., Kääriainen L., Lvov D. K., Porterfield J. S., Russell P. k. 1985; Flaviviridae. Intervirology 24:183–192
    [Google Scholar]
  29. Wright P. G., Warr H. M., Westaway E. G. 1983; Comparisons by peptide mapping of proteins specified by Kunjin, West Nile and Murray Valley encephalitis viruses. Australian Journal of Experimental Biology and Medical Science 61:641–653
    [Google Scholar]
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