1887

Abstract

Summary

Tobacco mesophyll protoplasts were transfected with tobacco mosaic virus (TMV) RNA in an electric field using a newly devised chamber constructed with gold-coated glass panel electrodes. Up to 95% of protoplasts subjected to several DC pulses (50 µs, 550 to 800 V/cm) while suspended in 0.5 -mannitol containing 10 µg/ml TMV RNA became infected. The treatment did not affect the viability of the protoplasts or their stability during 40 h of incubation.

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/content/journal/jgv/10.1099/0022-1317-67-9-2037
1986-09-01
2019-10-15
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http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-9-2037
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