1887

Abstract

Summary

Tobacco mesophyll protoplasts were transfected with tobacco mosaic virus (TMV) RNA in an electric field using a newly devised chamber constructed with gold-coated glass panel electrodes. Up to 95% of protoplasts subjected to several DC pulses (50 µs, 550 to 800 V/cm) while suspended in 0.5 -mannitol containing 10 µg/ml TMV RNA became infected. The treatment did not affect the viability of the protoplasts or their stability during 40 h of incubation.

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/content/journal/jgv/10.1099/0022-1317-67-9-2037
1986-09-01
2021-10-25
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References

  1. Aoki S., Takebe I. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus ribonucleic acid. Virology 39:439–448
    [Google Scholar]
  2. Bates G. W., Gaynor J. J., Shekhawat N. S. 1983; Fusion of plant protoplasts by electric fields. Plant Physiology 72:1110–1113
    [Google Scholar]
  3. Fraenkel-Conrat H., Singer B., Tsugita A. 1961; Purification of viral RNA by means of bentonite. Virology 14:54–58
    [Google Scholar]
  4. Fromm M., Taylor L. P., Walbot V. 1985; Expression of genes transferred into monocot and dicot plant cells by electroporation. Proceedings of the National Academy of Sciences, U.S.A 82:5824–5828
    [Google Scholar]
  5. Huber R., Rezelman G., Hibi T., Van Kammen A. 1977; Cowpea mosaic virus infection of protoplasts from Samsun tobacco leaves. Journal of General Virology 34:315–323
    [Google Scholar]
  6. Langridge W. H. R., Li B. J., Szalay A. A. 1985; Electric field mediated stable transformation of carrot protoplasts with naked DNA. Plant Cell Reports 4:355–359
    [Google Scholar]
  7. Neumann E., Schaefer-Ridder M., Wang Y., Hofschneider P. H. 1982; Gene transfer into mouse lymphoma cells by electroporation in high electric fields. EMBO Journal 1:841–845
    [Google Scholar]
  8. Nishiguchi M., Langridge W. H. R., Szalay A. A., Zaitlin M. 1986; Electroporation-mediated infection of tobacco leaf protoplasts with tobacco mosaic virus RNA and cucumber mosaic virus RNA. Plant Cell Reports 5:57–60
    [Google Scholar]
  9. Nozu Y., Okada Y. 1968; Amino acid sequence of a common Japanese strain of tobacco mosaic virus. Journal of Molecular Biology 35:643–646
    [Google Scholar]
  10. Otsuki Y., Takebe I. 1969; Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplasts. Virology 38:497–499
    [Google Scholar]
  11. Potrykus I., Shillito R. D., Saul M. W., Paszkowski J. 1985; Direct gene transfer: state of the art and future potential. Plant Molecular Biology Reporter 3:117–128
    [Google Scholar]
  12. Shillito R. D., Saul M. W., Muller M., Potrykus I. 1985; High efficiency direct gene transfer to plants. Biol Technology 3:1099–1103
    [Google Scholar]
  13. Takebe I., Otsuki Y. 1969; Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proceedings of the National Academy of Sciences, U.S.A 64:843–848
    [Google Scholar]
  14. Watts J. W., King J. M. 1984; A simple method for large-scale electrofusion and culture of plant protoplasts. Bioscience Reports 4:335–342
    [Google Scholar]
  15. Zachrisson A., Bornman C. H. 1984; Application of electric field fusion in plant tissue culture. Physiologia Plantarum 61:314–320
    [Google Scholar]
  16. Zimmermann U. 1982; Electric field-mediated fusion and related electrical phenomena. Biochimica et biophysica acta 694:227–277
    [Google Scholar]
  17. Zimmermann U., Scheurich P. 1981; High frequency fusion of plant protoplasts by electric fields. Planta 151:26–32
    [Google Scholar]
  18. Zimmermann U., Scheurich P., Pilwat G., Benz R. 1981; Cells with manipulated functions: new perspectives for cell biology, medicine and technology. Angewandte Chemie: International Edition in English 20325–344
    [Google Scholar]
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