1887

Abstract

Summary

Herpes simplex virus type 1 and a fluorescein-labelled lectin (wheat germ agglutinin) were selectively transported to nerve cell bodies located in the inner compartment of a two-chamber tissue culture system after the application of virus or lectin to the neuritic processes in the outer culture compartment. Taxol, which stabilizes and alters intracellular arrangements of microtubules, and nocodazole, which disrupts microtubules, both inhibited this retrograde axonal transport of viral particles and lectin. The transport was also inhibited by erythro-9-3-(2-hydroxynonyl)adenine (EHNA), which blocks ATPases. However, EHNA was also an effective inhibitor of infection with the virus in non-neuronal cells (GMK AH-1). The nature of the action(s) of EHNA on neuritic transport of the virus is therefore less clear.

Keyword(s): axonal transport , HSV-1 and microtubules
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/content/journal/jgv/10.1099/0022-1317-67-9-2023
1986-09-01
2022-01-17
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References

  1. Adams R. J., Bbray P. 1983; Rapid transport of foreign particles microinjected into crab axons. Nature, London 303:718–720
    [Google Scholar]
  2. Batterson W., Furlong D., Roizman B. 1983; Molecular genetics of herpes simplex virus. VII. Further characterization of a ts mutant defective in release of viral DNA and in other stages of viral reproductive cycle. Journal of Virology 45:397–407
    [Google Scholar]
  3. Beckerle M. C. 1984; Microinjected fluorescent polystyrene beads exhibit saltatory motion in tissue culture cells. Journal of Cell Biology 98:2126–2132
    [Google Scholar]
  4. Bouchard P., Penningroth S. M., Cheung A., Gagnon C., Bardin C. W. 1981; Erythro-9-3-(2-hydroxynonyl)adenine is an inhibitor of sperm motility that blocks dynein ATPase and protein carboxylmethylase activities. Proceedings of the National Academy of Sciences, U.S.A. 78:1033–1036
    [Google Scholar]
  5. Forman D., Brown K. J., Promersberger M. E. 1983; Selective inhibition of retrograde axonal transport by erythro-9-3-(2-hydroxynonyl)adenine. Brain Research 272:194–197
    [Google Scholar]
  6. Goldberg P. J. 1982; Microinjection into an identified axon to study the mechanism of fast axonal transport. Proceedings of the National Academy of Sciences, U.S.A 79:4818–4822
    [Google Scholar]
  7. Gonatas N. K. 1979; Immunochemistry and receptors: studies on the redistribution and adsorptive endocytosis of antiimmunoglobulin antibodies, cholera toxin, and lectins. Progress in Neuropathology 4:51–60
    [Google Scholar]
  8. Herman B., Albertini D. 1984; A time-lapse video image intensification analysis of cytoplasmic organelle movements during endosome translocation. Journal of Cell Biology 98:565–576
    [Google Scholar]
  9. Herman B., Langevin M. A., Albertini D. F. 1983; The effects of taxol on the organization of the cytoskeleton in cultured ovarian granulosa cells. European Journal of Cell Biology 31:34–45
    [Google Scholar]
  10. Lycke E., Kristensson K., Svennerholm B., Vahlne A., Ziegler R. 1984; Uptake and transport of herpes simplex virus in neurites of rat dorsal root ganglia cells in culture. Journal of General Virology 65:55–64
    [Google Scholar]
  11. MÅansson J. -E., Olofsson S. 1983; Binding specificities of the lectins from Helix pomatia, soybean and peanut against different glycosphingolipids in liposome membranes. FEBS Letters 156:249–252
    [Google Scholar]
  12. Masurovsky E. B., Peterson E. R., Crain S. M., Horwitz S. B. 1981; Microtubule arrays in taxol-treated dorsal root ganglion-spinal cord cultures. Brain Research 217:392–398
    [Google Scholar]
  13. Röytt ä M., Horwitz S. B., Raine C. S. 1984; Taxol-induced neuropathy: short-term effects of local injection. Journal of Neurocytology 13:685–701
    [Google Scholar]
  14. Schiff P. B., Horwitz S. B. 1980; Taxol stabilizes microtubules in mouse fibroblast cells. Proceedings of the National Academy of Sciences, U.S.A 77:1561–1565
    [Google Scholar]
  15. Schnapp B. J., Vale R. D., Sheety M. P., Reese T. S. 1985; Single microtubules from squid axoplasm support bidirectional movement of organelles. Cel l 40:455–462
    [Google Scholar]
  16. Schroer T. A., Brady A. T., Kelly R. B. 1985; Fast axonal transport of foreign synaptic vesicles in squid axoplasm. Journal of Cell Biology 101:568–572
    [Google Scholar]
  17. Thoenen H., Kreutzberg G. W. 1981; The role of fast transport in the nervous system. Neurosciences Research Program Bulletin 20:1–138
    [Google Scholar]
  18. Vale R. D., Schnapp B. J., Reese T. S., Sheety M. P. 1985; Organelle, bead, and microtubule translocations promoted by soluble factors from the squid giant axon. Cel l 40:559–569
    [Google Scholar]
  19. Ziegler R. J., Herman R. E. 1980; Peripheral infection in culture of rat sensory neurons by herpes simplex virus. Infection and Immunity 28620–623
    [Google Scholar]
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