Genome fragments from two serotypes of barley yellow dwarf virus (BYDV) were cloned and used as hybridization probes. Dot blot assays using P-labelled plasmid probes readily detected BYDV in crude sap extracts from infected plants and were at least as sensitive as ELISA. Some clones were specific for either the RPV or the PAV serotypes. Others hybridized not only with the RNA of both serotypes but also with those of three other luteoviruses (beet western yellows, potato leaf roll and soybean dwarf), but they did not hybridize with the RNA of a tobamovirus or tRNA. These nucleic acid probes therefore have potential for the general diagnosis of infection by members of the luteovirus group as well as detection of specific BYDV serotype infections. The taxonomic implications of the observation that the genomes of different luteoviruses have some conserved regions are discused.

Keyword(s): BYDV , luteoviruses and probes

Article metrics loading...

Loading full text...

Full text loading...


Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error