1887

Abstract

Summary

In this study we show that vaccinia virus replication can be sensitive or resistant to interferon (IFN) in the same strain of mouse L cells. When IFN-treated L cells were maintained in suspension culture, infection led to a rapid inhibition of both viral and cellular protein synthesis together with breakdown of viral RNA and of rRNA. When IFN-treated L cells were maintained in monolayer culture, infection did not lead to significant inhibition of viral protein or RNA synthesis and breakdown of viral or of rRNA was not observed. The resistance of vaccinia virus replication to IFN was not dependent on the input multiplicity or state of growth of the cells (actively dividing or resting). Qualitative and quantitative differences in viral transcription and translation were observed between the two virus-cell systems. Our findings are consistent with the hypothesis that the sensitivity or resistance of vaccinia virus to IFN is mediated by specific viral products that act as activators or selective inhibitors of, at least, the dsRNA-dependent ppp(A2′p)A synthetase/RNase system.

Keyword(s): gene expression , IFN and vaccinia virus
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-4-801
1986-04-01
2021-08-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/4/JV0670040801.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-4-801&mimeType=html&fmt=ahah

References

  1. Bablanian R., Coppola G., Scribani S., Esteban M. 1981; Inhibition of protein synthesis by vaccinia virus. IV. The role of low molecular weight RNA in the inhibition of protein synthesis. Virology 112:13–24
    [Google Scholar]
  2. Benavente J., Paez E., Esteban M. 1984; Indiscriminate degradation of RNAs in interferon-treated, vaccinia virus-infected mouse L-cells. Journal of Virology 51:866–871
    [Google Scholar]
  3. Esteban M., Paez E. 1985; The 2–5A system and vaccinia virus. In The 2–5A System: Molecular and Clinical Aspects of the Interferon-regulated 2-5A Pathway vol 202 pp. 25–34 Edited by Williams B. R. G., Silverman R. H. New York: Alan R. Liss;
    [Google Scholar]
  4. Esteban M., Benavente J., Paez E. 1984; Effect of interferon on integrity of vaccinia virus and ribosomal RNA in infected cells. Virology 134:40–51
    [Google Scholar]
  5. Goswami B. B., Sharma O. 1984; Degradation of rRNA in interferon-treated vaccinia virus-infected cells. Journal of Biological Chemistry 259:1371–1374
    [Google Scholar]
  6. Lenk R., Penman S. 1979; The cytoskeletal framework and poliovirus metabolism. Cell 16:289–301
    [Google Scholar]
  7. Metz D., Esteban M. 1972; Interferon inhibits viral protein synthesis in L cells infected with vaccinia virus. Nature, London 238:385–388
    [Google Scholar]
  8. Pacha R. F., Condit R. C. 1985; Characterization of a temperature-sensitive mutant of vaccinia virus reveals a novel function that prevents virus-induced breakdown of RNA. Journal of Virology 56:395–403
    [Google Scholar]
  9. Paez E., Esteban M. 1984a; Resistance of vaccinia virus to interferon is related to an interference phenomenon between the virus and the interferon system. Virology 134:12–28
    [Google Scholar]
  10. Paez E., Esteban M. 1984b; Nature and mode of action of vaccinia virus products that block activation of the interferon-mediated ppp(A2′p)n A-synthetase. Virology 134:29–39
    [Google Scholar]
  11. Paez E., Esteban M. 1985; Interferon prevents the generation of spontaneous deletions at the left terminus of vaccinia DNA. Journal of Virology 56:75–84
    [Google Scholar]
  12. Paez E., Dallo S., Esteban M. 1985; Generation of a dominant 8 MDa deletion at the left terminus of vaccinia virus DNA. Proceedings of the National Academy of Sciences, U.S.A 82:3365–3369
    [Google Scholar]
  13. Rice A. P., Kerr I. M. 1984; Interferon-mediated, double-stranded RNA-dependent protein kinase is inhibited in extracts from vaccinia virus-infected cells. Journal of Virology 50:229–236
    [Google Scholar]
  14. Rice A. P., Roberts W. K., Kerr I. M. 1984; 2-5A accumulates to high levels in interferon-treated, vaccinia virus-infected cells in the absence of any inhibition of virus replication. Journal of Virology 50:220–228
    [Google Scholar]
  15. Silverman R. H., Cayley P. J., Knight M., Gilbert C. S., Kerr I. M. 1982; Control of the ppp(A2'p)nA system in HeLa cells: effects of interferon and virus infection. European Journal of Biochemistry 124:131–138
    [Google Scholar]
  16. Von venroolj W. J., Sillekens P. T. G., Von Eekelen A. G., Reinders R. J. 1981; On the association of mRNA with the cytoskeleton in uninfected and adenovirus infected human KB cells. Experimental Cell Research 135:79–91
    [Google Scholar]
  17. Weir J. P., Moss B. 1984; Regulation of expression and nucleotide sequence of a late vaccinia virus gene. Journal of Virology 51:662–669
    [Google Scholar]
  18. Whitaker-Dowling P., Youngner J. S. 1983; Vaccinia rescue of VSV from interferon-induced resistance: reversal of translation block and inhibition of protein kinase activity. Virology 131:128–136
    [Google Scholar]
  19. Whitaker-Dowling P., Youngner J. S. 1984; Characterization of a specific kinase inhibitory factor produced by vaccinia virus which inhibits the interferon induced protein kinase. Virology 137171–181
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-67-4-801
Loading
/content/journal/jgv/10.1099/0022-1317-67-4-801
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error