1887

Abstract

Summary

Two strains of bluetongue virus (BTV) serotype 11, UC-2 and UC-8, were identified by the electrophoretic migration pattern of their genomic RNA segments on polyacrylamide gel electrophoresis. Significant differences in virulence of these two viruses could be demonstrated by subcutaneous inoculation of newborn mice. No signs of disease were observed in mice infected with UC-2. Mice infected with UC-8 died of a severe necrotizing encephalitis, which resembled lesions in bovine and ovine foetuses infected with BTV.

Keyword(s): BTV , murine , orbivirus and virulence
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-4-765
1986-04-01
2024-12-03
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/4/JV0670040765.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-4-765&mimeType=html&fmt=ahah

References

  1. Barber T. L., Collisson E. W. 1983; Implications of a new bluetongue serotype for the U,. S. livestock industry. Proceedings of the 87th Annual Meeting of the United States Animal Health Association pp. 90–104
    [Google Scholar]
  2. Della-Porta A. J., Mcphee D. A., Parsonson I. M., Snowdon W. A. 1982; Classification of the orbiviruses. Confusion in the use of terms bluetongue virus, bluetongue-like virus, bluetongue-related virus and the overall nomenclature. Australian Veterinary Journal 58:164–165
    [Google Scholar]
  3. Foster N. M., Luedke A. J. 1968; Direct assay for bluetongue virus by intravascular inoculation of embryonating chicken eggs. American Journal of Veterinary Research 29:749–753
    [Google Scholar]
  4. Goldsmit L., Barzilai E. 1968; An improved method for the isolation and identification of bluetongue virus by intravenous inoculation of embryonating chicken eggs. Journal of Comparative Pathology 78:477–487
    [Google Scholar]
  5. Gorman B. M. 1983; On the evolution of orbiviruses. Intervirology 20:169–180
    [Google Scholar]
  6. Jeggo M. H., Wardley R. C. 1982; Generation of cross-reactive cytotoxic T lymphocytes following immunization of mice with various bluetongue virus types. Immunology 45:629–635
    [Google Scholar]
  7. Knudson D. L., Shope R. E. 1985; Overview of the orbiviruses. In Bluetongue and Related Orbiviruses pp. 255–266 Edited by Barber T. L., Jochim M. M. New York: Alan R. Liss;
    [Google Scholar]
  8. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  9. Letchworth G. J., Appleton J. A. 1983; Passive protection of mice and sheep against bluetongue virus by a neutralizing monoclonal antibody. Infection and Immunity 39:208–212
    [Google Scholar]
  10. McLachlan N. J., Osburn B. I. 1983; Bluetongue virus-induced hydranencephaly in cattle. Veterinary Pathology 20:563–573
    [Google Scholar]
  11. Narayan O., Johnson R. T. 1972; Effects of viral infection on nervous system development. American Journal of Pathology 68:1–14
    [Google Scholar]
  12. Osburn B. I., Silverstein A. M., Prendergast R. A., Johnson R. T., Parshall C. J. 1971; Experimental viral- induced congenital encephalopathies. I. Pathology of hydranencephaly and porencephaly caused by bluetongue vaccine virus. Laboratory Investigation 25:197–205
    [Google Scholar]
  13. Reed L. J., Muench H. 1938; A simple method of estimating fifty percent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  14. Richards W. P. C., Cordy D. R. 1967; Bluetongue virus infection: pathologic responses of nervous systems in sheep and mice. Science 156:530–531
    [Google Scholar]
  15. Sammons D. W., Adams L. D., Nishizawa E. E. 1981; Ultrasensitive silver-based color staining of polypeptides in polyacrylamide gels. Electrophoresis 2:135–141
    [Google Scholar]
  16. Squire K. R. E., Chuang R. Y., Osburn B. I., Knudson D. L., Doi R. H. 1983; Rapid methods for comparing the double-stranded RNA genome profiles of bluetongue virus. Veterinary Microbiology 8:543–553
    [Google Scholar]
  17. Stott J. L., Barber T. L., Osburn B. I. 1978; Serotyping bluetongue virus: a comparison of plaque inhibition (disc) and plaque neutralization methods. Proceedings of the 21st Annual Meeting of the American Association of Veterinary Laboratory Diagnosticians pp. 399–410
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-67-4-765
Loading
/content/journal/jgv/10.1099/0022-1317-67-4-765
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error