1887

Abstract

Summary

Oral inoculation of newborn mice with the MET strain of human rotavirus produced transient diarrhoeal disease. Light and scanning electron microscopy showed typical rotavirus-induced morphological lesions in the villous epithelium of the small intestine consisting of extensive cytoplasmic vacuolation, villous necrosis and atrophy. Virus recovered from intestinal suspensions of infected mice showed the typical electrophoretic profile of the genome of the inoculated strain. Rotavirus antibody appeared in infected mice 10 to 20 days after inoculation but not in controls or nursing dams. The availability of a small animal model for experimental infection with human rotaviruses should prove useful for virulence and protection studies.

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/content/journal/jgv/10.1099/0022-1317-67-3-577
1986-03-01
2021-08-05
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References

  1. Estes M. K., Palmer E. L., Obijeski J. F. 1983; Rotaviruses: a review. Current Topics in Microbiology and Immunology 102:123–184
    [Google Scholar]
  2. Eydeloth R. S., Vonderfecht S. L., Sheridan J. F., Enders L. D., Yolken R. H. 1984; Kinetics of viral replication and local and systemic immune responses in experimental rotavirus infection. Journal of Virology 50:947–950
    [Google Scholar]
  3. Gerna G., Battaglia M., Milenesi G., Passarani N., Percivalle E., Cattaneo E. 1984; Serotyping of cell culture-adapted subgroup 2 human rotavirus strains by neutralization. Infection and Immunity 43:722–729
    [Google Scholar]
  4. Greenberg H. G., Kalica A. R., Wyatt R. G., Jones R. W., Kapikian A. Z., Chanock R. M. 1981; Rescue of non-cultivable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivable bovine rotavirus. Proceedings of the National Academy of Sciences. U,. S,. A 78:420–424
    [Google Scholar]
  5. Little L. M., Shadduck J. A. 1982; Pathogenesis of rotavirus infection in mice. Infection and Immunity 38:755–763
    [Google Scholar]
  6. Offit P. A., Clark H. F., Kornstein M. J., Plotkin S. A. 1984; A murine model for oral infection with a primate rotavirus (simian SA11). Journal of Virology 51:233–236
    [Google Scholar]
  7. Pereira H. G., Azeredo R. S., Leite J. P. G., Barth O. M., Sutmoller F., De Farias V., Vidal M. N. P. 1983; Comparison of polyacrylamide gel electrophoresis (PAGE), immuno-electron microscopy (IEM) and enzyme immunoassay (EIA) for the rapid diagnosis of rotavirus infection in children. Memorias do Instituto Oswaldo Cruz 78:483–490
    [Google Scholar]
  8. Riepenhoff Talty M., Lee P. C., Carmody P. J., Barrett H. J., Ogra P. L. 1982; Age-dependent rotavirus-enterocyte interactions. Proceedings of the Society for Experimental Biology and Medicine 170:146–164
    [Google Scholar]
  9. Sheridan J. F., Eydeloth R. S., Vonderfecht S. L., Aurelian L. 1983; Virus specific immunity in neonatal and adult mouse rotavirus infection. Infection and Immunity 39:917–927
    [Google Scholar]
  10. Wolf J. L., Cukor G., Blacklow N. R., Dambrauskas R., Trier J. S. 1981; Susceptibility of mice to rotavirus infection: effects of age and administration of corticosteroids. Infection and Immunity 33:565–574
    [Google Scholar]
  11. Wyatt R. G., James W. D., Bohl E. H., Theil R. W., Saif L. J., Kalica A. R., Greenberg H. B., Kapikian A. Z., Chanock R. M. 1980; Human rotavirus type 2: cultivation in vitro. Science 207:189–191
    [Google Scholar]
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