1887

Abstract

Summary

Amino acid sequences coded for by the preS region of the hepatitis B virus (HBV) envelope gene are present both in HBV and in subviral hepatitis B surface antigen (HBsAg) particles. Consequently, anti-preS-specific antibodies are elicited during the course of HBV infection. Such antibodies are virus-neutralizing. Therefore, it is important to determine whether or not vaccination with HBsAg also induces an anti-preS-specific immune response. We describe here an enzyme-linked immunosorbent assay applicable for the screening of sera from vaccinated individuals for anti-preS antibodies. IgG from serum specimens was adsorbed to staphylococcal Protein A on a superparamagnetic support and subsequently mixed with a synthetic peptide analogue [preS(120–145)] covalently linked to β-lactamase. The presence of anti-preS in serum specimens resulted in binding of the conjugated β-lactamase to the magnetic support. The adsorbed enzyme was quantified colorimetrically.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-3-453
1986-03-01
2024-10-12
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/3/JV0670030453.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-3-453&mimeType=html&fmt=ahah

References

  1. Charnay P., Mandart E., Hampe A., Fitoussi F., Tiollais P., Galibert F. 1979; Localization of the viral genome and nucleotide sequence of the gene coding for the major polypeptide of the hepatitis B surface antigen (HBsAg). Nucleic Acids Research 7:335–346
    [Google Scholar]
  2. Coursaget P., Barres J. L., Chiron J. P., Adamowicz P. 1985; Hepatitis B vaccines with and without polymerized albumin receptors. Lancet i:1152–1153
    [Google Scholar]
  3. Desmyter J., Colaert J. 1984; Comparative immunogenicity of MSD, Pasteur and CLB hepatitis B vaccines in 245 hemodialysis patients. In Viral Hepatitis and Liver Disease pp. 709–710 Edited by Vyas G. N. Dienstag J. L. Hoofnagle J. H. Orlando: Grune & Stratton;
    [Google Scholar]
  4. Desmyter J., Colaert J., De Groote G., Reynders M., Reerink-Brongers E. E., Lelie P. N., Dees P. J., Reesink H. W. 1983; Efficiency of heat-inactivated hepatitis B vaccine in haemodialysis patients and staff. Lancet ii:1323–1328
    [Google Scholar]
  5. Dreesman G. R., Hollinger F. B., Mccombs R. M., Melnick J. L. 1973; Alteration of hepatitis B antigen (HB Ag) determinants by reduction and alkylation. Journal of General Virology 19:129–134
    [Google Scholar]
  6. Guesry P. R., Adamowicz P., Jungers P., Courouce A.-M., Laplanche A., Lacour B., Benhamou E., Degos F., Crosnier J. 1982; Vaccination against hepatitis B in high-risk hemodialysis units: a double-blind study. In Viral Hepatitis pp. 493–507 Edited by Szmuness W., Alter H. J., Maynard J. E. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  7. Heermann K. H., Goldmann U., Schwartz W., Seyffarth T., Baumgarten H., Gerlich W. H. 1984; Largesurface proteins of hepatitis B virus containing the pre-S sequence. Journal of Virology 52:396–402
    [Google Scholar]
  8. Hilleman M. R., Buynak E. B., Mcaleer W. J., Mclean A. A., Provost P. J., Tytell A. A. 1982; Hepatitis A and hepatitis B vaccines. In Viral Hepatitis pp. 385–398 Edited by Szmuness W., Alter H. J., Maynard J. E. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  9. Kent S. B. H., Neurath A. R. 1985; Occurrence of HBV-coded proteins in the viral envelope: role in the human immune response. Hepatology (in press)
    [Google Scholar]
  10. Kent S., Neurath A. R., Aebersold R., PARKER K., Strick N. 1985; Epitope scanning: strategies for determining the precise location of continuous epitopes applied to the pre-S region of hepatitis B virus. In Modern Approaches to Vaccines p. 99 Edited by Chanock R., Lerner R., Brown F. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Lerner R. A. 1982; Tapping the immunological repertoire to produce antibodies of predetermined specificity. Nature, London 299:592–596
    [Google Scholar]
  12. Mcauliffe V. J., Purcell R. H., Gerin J. L., Tyeryar F. J. 1982; Current status of the NIAID hepatitis B vaccines. In Viral Hepatitis pp. 425–435 Edited by Szmuness W., Alter H. J. Maynard J. E. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  13. Machida A., Kishimoto S., Ohnuma H., Baba K., Ito Y., Miyamoto H., Funatsu G., Oda K., Usuda S., Togami S., Nakamura T., Miyakawa Y., Mayumi M. 1984; A polypeptide containing 55 amino acid residues coded by the pre-S region of hepatitis B virus deoxyribonucleic acid bears the receptor for polymerized human as well as chimpanzee albumins. Gastroenterology 86:910–918
    [Google Scholar]
  14. Michel M. -L., Pontisso P., Sobczak E., Malpiece Y., Streeck R. E., Tiollais P. 1984; Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin. Proceedings of the National Academy of Sciences, U.S.A 81:7708–7712
    [Google Scholar]
  15. Milich D. R., Thornton G. B., Neurath A. R., Kent S. B. H., Michel M. -L., Tiollais P., Chisari F. V. 1985; Enhanced immunogenicity of the pre-S region of hepatitis B surface antigen. Science 228:1195–1199
    [Google Scholar]
  16. Neurath A. R., Kent S. B. H. 1985; Antigenic structure of human hepatitis viruses. In Immunochemistry of Viruses: The Basis for Serodiagnosis and Vaccines pp. 325–366 Edited by V M. H. Regenmortel Van, Neurath A. R. Amsterdam: Elsevier;
    [Google Scholar]
  17. Neurath A. R., Trepo C., Chen M., Prince A. M. 1976; Identification of additional antigenic sites on Dane particles and the tubular forms of hepatitis B surface antigen. Journal of General Virology 30:277–285
    [Google Scholar]
  18. Neurath A. R., Kent S. B. H., Strick N. 1984a; Location and chemical synthesis of a pre-S gene coded immunodominant epitope of hepatitis B virus. Science 224:392–395
    [Google Scholar]
  19. Neurath A. R., Rent S. B. H., Strick N. 1984b; Monoclonal antibodies to hepatitis B surface antigen (HBsAg) with anti-a specificity recognize a synthetic peptide analogue (S135–155) with unmodified lysine (141). Journal of Virological Methods 9:341–346
    [Google Scholar]
  20. Neurath A. R., Kent S. B. H., Strick N., Taylor P., Stevens C. E. 1985a; Hepatitis B virus contains pre-S gene encoded domains. Nature, London 315:154–156
    [Google Scholar]
  21. Neurath A. R., Kent S. B. H., Strick N. 1985b; Synthetic peptides in immunoprophylaxis and diagnosis of hepatitis B.. In Proceedings of the Labsystems Research Symposium on Synthetic Peptides in Biology and Medicine –(Hameenlinna, Finland) Amsterdam: Elsevier; (in press)
    [Google Scholar]
  22. Neurath A. R., Strick N., Sproul P., Baker L., Rubinstein P., Stevens C. E., Taylor P., Gallo R. C., Gold J. W. M., Lee Y. S., Nilsen T. 1985c; Radioimmunoassay and enzyme-linked immunoassay of antibodies to the core protein (P24) of human T-lymphotropic virus (HLTV-III). Journal of Virological Methods II:75–86
    [Google Scholar]
  23. Neurath A. R., Kent S. B. H., Strick N., Stark D., Sproul P. 1985d; Genetic restriction of immune responsiveness to synthetic peptides corresponding to sequences in the preS region of the hepatitis B virus (HBV) envelope gene. Journal of Medical Virology 17:119–125
    [Google Scholar]
  24. Neurath A. R., Kent S. B. H., Parker K., Prince A. M., Strick N., Brotman B., Sproul P. 1985e; Antibodies to a synthetic peptide from the preS 120–145 region of the hepatitis B virus are virus-neutralizing. Vaccine (in press)
    [Google Scholar]
  25. Neurath A. R., Strick N., Kent S. B. H., Offensperger W., Wahl S., Christman J. K., Acs G. 1985f; Enzyme- linked immunoassay of pre-S gene-coded sequences in hepatitis B vaccines. Journal of Virological Methods (in press)
    [Google Scholar]
  26. Offensperger W., Wahl S., Neurath A. R., Price P., Strick N., Kent S. B. H., Christman J. K., Acs G. 1985; Expression in E. colioi a cloned DNA sequence encoding the preS2 region of hepatitis B virus. Proceedings of the National Academy of Sciences, U.S.A. 82:7540–7544
    [Google Scholar]
  27. Okamoto H., Imai M., Usuda S., Tanaka B., Tachibana K., Mishiro S., Machida A., Nakamura T., Miyakawa Y., Mayumi M. 1985; Hemagglutination assay of polypeptide coded by the pre-S region of hepatitis B virus DNA with monoclonal antibody: correlation of pre-S polypeptide with the receptor for polymerized human serum albumin in serums containing hepatitis B antigens. Journal of Immunology 134:1212–1216
    [Google Scholar]
  28. Persing D. H., Varmus H. E., Ganem D. 1985; A frameshift mutation in the pre-S region of the human hepatitis B virus genome allows production of surface antigen particles but eliminates binding to polymerized albumin. Proceedings of the National Academy of Sciences, U.S.A 82:3440–3444
    [Google Scholar]
  29. Peterson D. L., Roberts I. M., Vyas G. N. 1977; Partial amino acid sequence of two major component polypeptides of hepatitis B surface antigen. Proceedings of the National Academy of Sciences, U.S.A 74:1530–1534
    [Google Scholar]
  30. Reerink-Brongers E. E., Reesnik H. W., Brummelhuis H. G. J., Schut B. J. T., Dees P. J., Lelie P. N., Raap A. K., Wilson-De Sturler L. A., Van Aken W. G., Balner H., Van Eerd P. M. C. A., Van Schie T. C., Stitz L. W., Van Steenis B., Feltkamp-Vroom T. M. 1982; Preparation and evaluation of heat-inactivated HBsAg as a vaccine against hepatitis B,. In Viral Hepatitis pp. 437–450 Edited by Szmuness W., Alter H. J., Maynard J. E. Philadelphia: Franklin Institute Press;
    [Google Scholar]
  31. Stevens C. E., Taylor P. E., Tong M. J., Toy P. T., Vyas G. N. 1984a; Hepatitis B vaccine: an overview. In Viral Hepatitis and Liver Disease pp. 275–291 Edited by Vyas G. N., Dienstag J. L. Hoofnagle J. H. Orlando: Grune & Stratton;
    [Google Scholar]
  32. Stevens C. E., Alter H. J., Taylor P. E., Zang E. A., Harley E. J., Szmuness W. 1984b; Hepatitis B vaccine in patients receiving hemodialysis. Immunogenicity and efficacy. New England Journal of Medicine 311:496–501
    [Google Scholar]
  33. Sukeno N., Shirachi R., Yamaguchi J., Ishida N. 1972; Reduction and reoxidation of Australia antigen: loss and reconstitution of particle structure and antigenicity. Journal of Virology 9:182–183
    [Google Scholar]
  34. Tiollais P., Charnay P., Vyas G. N. 1981; Biology of hepatitis B virus. Science 213:406–111
    [Google Scholar]
  35. Tiollais P., Pourcel C., Dejean A. 1985; The hepatitis B virus. Nature, London 317:489–495
    [Google Scholar]
  36. Vyas G. N., Rao K. R., Ibrahim A. B. 1972; Australia antigen (hepatitis B antigen): a conformational antigen dependent on disulfide bonds. Science 178:1300–1301
    [Google Scholar]
  37. Wong D. T., Nath N., Sninsky J. J. 1985; Identification of hepatitis B virus polypeptides encoded by the entire pre-S open reading frame. Journal of Virology 55:223–231
    [Google Scholar]
  38. Yolken R. H., Wee S.-B., Van Regenmortel M. 1984; The use of beta-lactamase in enzyme immunoassays for detection of microbial antigens. Journal of Immunological Methods 73:109–123
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-67-3-453
Loading
/content/journal/jgv/10.1099/0022-1317-67-3-453
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error