1887

Abstract

Summary

Double-stranded RNA (dsRNA) has been isolated from bananas infected with banana bunchy top disease (BBTD) but not from healthy bananas of the same cultivar. Four major dsRNA species were present in seven different extracts and these had molecular weights of 4.40 × 10, 1.35 × 10, 0.50 × 10 and 0.48 × 10. This pattern of dsRNA species is similar to that in extracts from plants infected with barley yellow dwarf virus or beet western yellows virus strain ST9. The amount of extractable BBTD-specific dsRNA was affected by the temperature at which the infected plants were incubated and the interval between infection and harvest. The maximum amount of dsRNA was obtained from plants incubated at 30 °C and harvested 23 days after inoculation.

Keyword(s): banana bunchy top , dsRNA and luteovirus
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-67-2-371
1986-02-01
2024-10-08
Loading full text...

Full text loading...

/deliver/fulltext/jgv/67/2/JV0670020371.html?itemId=/content/journal/jgv/10.1099/0022-1317-67-2-371&mimeType=html&fmt=ahah

References

  1. Dawson W. O., Dodds J. A. 1982; Characterization of sub-genomic double-stranded RNAs from virus-infected plants. Biochemical and Biophysical Research Communications 107:1230–1235
    [Google Scholar]
  2. Dodds J. A., Morris T. J., Jordan R. L. 1984; Plant viral double-stranded RNA. Annual Review of Phytopathology 22:151–168
    [Google Scholar]
  3. Falk B. W., Duffus J. E. 1984; Identification of small single- and double-stranded RNAs associated with severe symptoms in beet western yellows virus-infected Capsella hursa-pastoris. Phytopathology 74:1224–1229
    [Google Scholar]
  4. Gildow F. E., Ballinger N. E., Rochow W. F. 1983; Identification of double-stranded RNAs associated with barley yellow dwarf virus infection of oats. Phytopathology 73:1570–1572
    [Google Scholar]
  5. Grill L. K., Garger S. J. 1981; Identification and characterization of double-stranded RNA associated with cytoplasmic male sterility in Vicia faba. Proceedings of the National Academy of Sciences, U,. S,. A. 78:7043–7046
    [Google Scholar]
  6. Lkegami M., Fraenkel-Conrat H. 1979; Characterization of double-stranded ribonucleic acid in tobacco leaves. Proceedings of the National Academy of Sciences, U.S.A 76:3637–3640
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. Magee C. J. P. 1940; Transmission studies on the banana bunchy-top virus. Journal of the Australian Institute of Agricultural Science 6:109–110
    [Google Scholar]
  9. Matthews R. E. F. 1982; Classification and nomenclature of viruses. Intervirology 17:1–200
    [Google Scholar]
  10. Merril C. F., Goldman D., Sedman S. A., Ebert M. H. 1981; Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins. Science 211:1437–1438
    [Google Scholar]
  11. Morris T. J., Dodds J. A. 1979; Isolation and analysis of double-stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858
    [Google Scholar]
  12. Reddy D. V. R., Boccardo G., Outridge R., Teakle D. S., Black L. M. 1975; Electrophoretic separation of dsRNA genome segments from Fiji disease and maize rough dwarf viruses. Virology 63:287–291
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-67-2-371
Loading
/content/journal/jgv/10.1099/0022-1317-67-2-371
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error