1887

Abstract

Summary

Oligo-2′,5′-adenylate synthetase activity was investigated in several human lymphoblastoid cell lines of B cell origin treated with reagents having effects on gene expression, 5-azacytosine (5AZct), 5-azacytidine (5AZcd) and 5-iodo-2′-deoxyuridine (IUdR). Enzyme activity did not increase in cells treated with 5AZcd, but the other reagents induced significant levels of activity. Interferon (IFN) activity was not detected in culture fluids of cells treated with 5AZcd or 5AZct. On the other hand, treatment of cells with IUdR led to the production of 5 to 10 units/ml IFN by NC-37 and Raji cells, but not by other human B cell lines. Enzyme induction by IUdR occurred in IFN-producing cells, NC-37 and Raji, exposed to anti-IFN-α sera and also in IFN-non-producing human B cells. The effect of 5AZct on enzyme induction was observed only after cultivation of cells for more than 1 month. This compound could not induce enzyme activity in Epstein-Barr virus (EBV)-negative cells, BJAB, but only in the EBV-positive cell lines NC-37 and Raji. In contrast, treatment of cells with IUdR for only 3 days induced the enzyme in all human lymphoblastoid cell lines of B cell origin except P3HR-1, but not in other human cell lines, FL, HeLa and K562. Oligo-2′,5′-adenylate synthetase activity in P3HR-1 cells, which produce EBV particles, was hardly affected by IUdR or IFN treatment.

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1986-11-01
2022-01-23
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References

  1. Baker P. N., Bradshaw T. K., Morser J., Burke D. C. 1979; The effect of 5-bromodeoxyuridine on interferon production in human cells. Journal of General Virology 45:177–184
    [Google Scholar]
  2. Baker P. N., Morser J., Burke D. C. 1980; Effect of sodium butyrate on a human lymphoblastoid cell line (Namalwa) and its interferon production. Journal of Interferon Research 1:71–77
    [Google Scholar]
  3. Ball A. L. 1982; 2′, 5′-O1igoadenylate synthetase. In The Enzymes vol 15 pp 281–313 Edited by Boyer P. D. New York: Academic Press;
    [Google Scholar]
  4. Besancon F., Bourgeade M. F., Justesen J., Ferbus D., Thang M. N. 1981; Two inducers of cell differentiation enhance the 2′, 5′-oligoadenylate synthetase activity in MSV transformed cells. Biochemical and Biophysical Research Communications 103:16–24
    [Google Scholar]
  5. Christman J. K., Mendelsohn N., Herzog D., Schneiderman N. 1983; Effect of 5-azacytidine on differentiation and DNA methylation in human promyelocytic leukemia cells (HL-60). Cancer Research 43:763–769
    [Google Scholar]
  6. Creager R. S., Whitaker-Dowling P., Frey T. K., Youngner J. S. 1982; Varied responses of human B-lymphoblastoid cell lines to infection with vesicular stomatitis virus. Virology 121:414–419
    [Google Scholar]
  7. Creasey A. A., Eppstein D. A., Marsh Y. V., Khan Z., Merigan T. C. 1983; Growth regulation of melanoma cells by interferon and (2′-5′)oligoadenylate synthetase. Molecular and Cellular Biology 3:780–786
    [Google Scholar]
  8. Creusot F., Acs G., Christman J. K. 1982; Inhibition of DNA methyltransferase and induction of Friend erythroleukemia cell differentiation by 5-azacytidine and 5-aza-2′-deoxycytidine. Journal of Biological Chemistry 257:2041–2048
    [Google Scholar]
  9. Dolei A., Colletta G., Capobianchi M. R., Rossi G. B., Vecchio G. 1980; Interferon effects on Friend leukaemia cells. I. Expression of virus and erythroid markers in untreated and dimethyl sulphoxide-treated cells. Journal of General Virology 46:227–236
    [Google Scholar]
  10. Fujii N., Minagawa T., Nakane A., Kato F., Ohno S. 1983; Spontaneous production ofγ-interferon in cultures of T lymphocytes obtained from patients with Behçet’s disease. Journal of Immunology 130:1683–1686
    [Google Scholar]
  11. Fujii N., Kotake S., Hirose S., Ohno S., Yasuda I., Sagawa K., Ishikawa K., Minagawa T. 1984; OligO-2′, 5′-adenylate synthetase activity in peripheral blood mononuclear leukocytes in various diseases. Journal of Clinical Microbiology 20:1216–1218
    [Google Scholar]
  12. Gerber P. 1972; Activation of Epstein-Barr virus by 5-bromodeoxyuridine in virus-free human cells. Proceedings of the National Academy of Sciences, U.S.A. 69:83–85
    [Google Scholar]
  13. Glough D. W., Kunkel L. M., Davidson R. L. 1982; 5-Azacytidine induced reactivation of a herpes simplex thymidine kinase gene. Science 216:70–73
    [Google Scholar]
  14. Groudine M., Eisenman R., Weintraub H. 1981; Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation. Nature, London 292:311–317
    [Google Scholar]
  15. Heremans H., Deley M., Volckaert-Vervliet G., Billiau A. 1980; Interferon production and virus replication in lymphoblastoid cells infected with different viruses. Archives of Virology 63:317–320
    [Google Scholar]
  16. Itkes A. V., Turpaev K. T., Kartasheva O. N., Vagonis A. I. 1982; Theophylline-induced 2′, 5′-oligo(A) synthetase in NIH 3T3 cells. Biochemistry International 5:15–21
    [Google Scholar]
  17. Jacobsen H., Krause D., Friedman R. M., Silverman R. H. 1983; Induction of ppp(A2′p)nA-dependent RNase in murine JLS-V9R cells during growth inhibition. Proceedings of the National Academy of Sciences, U.S.A. 80:4954–4958
    [Google Scholar]
  18. Jaenisch R., Schnieke A., Harbers K. 1985; Treatment of mice with 5-azacytidine efficiently activates silent retroviral genomes in different tissues. Proceedings of the National Academy of Sciences, U.S.A. 82:83–85
    [Google Scholar]
  19. Jonak G. J., Knight E. 1984; Selective reduction of c-myc mRNA in Daudi cells by humanα interferon. Proceedings of the National Academy of Sciences, U.S.A. 81:1747–1750
    [Google Scholar]
  20. Kikuta H., Mizuno F., Yano S., Osato T. 1984; Interferon production by Epstein-Barr virus in human mononuclear leukocytes. Journal of General Virology 65:837–841
    [Google Scholar]
  21. Kimchi A., Shure H., Revel M. 1979; Regulation of lymphocyte mitogenesis by (2′-5′)oligoisoadenylate. Nature, London 282:849–851
    [Google Scholar]
  22. Knight E., Anton E. D., Fahey D., Friedland B. K., Jonak G. J. 1985; Interferon regulates c-myc gene expression in Daudi cells at the post-transcriptional level. Proceedings of the National Academy of Sciences, U.S.A. 82:1151–1154
    [Google Scholar]
  23. Krishnan I., Baglioni C. 1980; Increased levels of (2′-5′)oligo(A) polymerase activity in human lymphoblastoid cells treated with glucocorticoid. Proceedings of the National Academy of Sciences, U.S.A. 77:6506–6510
    [Google Scholar]
  24. Leanderson T., Nordfelth R., Lundgren E. 1982; Anti-proliferative effect of (2′-5′)oligoadenylate distinct from that of interferon in lymphoid cells. Biochemical and Biophysical Research Communications 107:511–517
    [Google Scholar]
  25. Morser J., Meager A., Colman A. 1980; Enhancement of interferon mRNA levels in butyric acid treated Namalwa cells. FEBS Letters 112:203–206
    [Google Scholar]
  26. Niwa O., Yokota Y., Ishida H., Sugahara T. 1983; Independent mechanism involved in suppression of the Moloney leukemia virus genome during differentiation of murine teratocarcinoma cells. Cell 32:1105–1113
    [Google Scholar]
  27. Oikarinen J. 1982; Cortisol induces (2′, 5′)oligoadenylate synthetase in cultured chick embryo tendon fibroblast. Biochemical and Biophysical Research Communications 105:876–881
    [Google Scholar]
  28. Orlic D., Wu J. M., Carmichael R. D., Quaint F., Kobylack M., Gordon A. S. 1982; Increased erythropoiesis and 2′, 5′-A polymerase activity in the marrow and spleen of phenylhydrazine-injected rats. Experimental Hematology 10:478–485
    [Google Scholar]
  29. Schmidt A., Hattori T., Hoffman T. 1984; Differentiation of a human monocyte-like cell line by (2′-5′)oligoadenylate. Experimental Cell Research 150:292–297
    [Google Scholar]
  30. Shuttleworth J., Morser J., Burke D. C. 1982; Control of interferon mRNA levels and interferon yields in butyrate and 5′-bromodeoxyuridine-treated Namalwa cells. Journal of General Virology 58:25–35
    [Google Scholar]
  31. Smekens M. E., Vandenbussche P., Content J., Dumont J. E. 1983; (2′-5′)o1igoadenylate in rat liver: modulation after partial hepatectomy. Proceedings of the National Academy of Sciences, U.S.A. 80:4609–4613
    [Google Scholar]
  32. Tomita Y., Cantell K., Kuwata T. 1982; Effect of human a-interferon on cell growth, replication of virus and induction of 2′-5′ oligoadenylate synthetase in three human lymphoblastoid cell lines and K562 cells. International Journal of Cancer 30:161–165
    [Google Scholar]
  33. Tovey M. G., Begon-Lours J., Gresser I., Morris A. G. 1977; Marked enhancement of interferon production in 5-bromodeoxyuridine treated human lymphoblastoid cell. Nature, London 267:455–457
    [Google Scholar]
  34. Yamamoto N., Mueller-Lantzsch N., Zur Hausen H. 1980; Effect of actinomycin D and cycloheximide on Epstein-Barr virus early antigen induction in lymphoblastoid cells. Journal of General Virology 51:255–261
    [Google Scholar]
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