1887

Abstract

Summary

With a reassortant from a cross of human rotavirus DS-1 (serotype 2) and OSU (serotype 5) it was determined that the OSU major neutralization glycoprotein antigen (VP7) was encoded by gene segment 9. A full-sized cloned cDNA copy of the OSU gene 9 was produced and sequenced. Hybridization of such labelled cDNA with the corresponding segment of a reassortant DS-1 × OSU virus confirmed the coding assignment. Comparison of the deduced amino acid sequence of the VP7 of OSU with those previously determined for five other rotavirus strains, representing four distinct serotypes, revealed some hydrophilic regions that exhibited significant homology and other hydrophilic domains with greater amino acid divergence. In one of the latter hydrophilic domains each of the five serotypes had a distinct amino acid substitution at residue 146, suggesting that it may be involved in serotype specificity.

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1986-11-01
2022-01-25
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References

  1. Arias C. F., Lopez S., Bell J. R., Strauss J. H. 1984; Primary structure of the neutralization antigen of simian rotavirus SA11 as deduced from cDNA sequence. Journal of Virology 50:657–661
    [Google Scholar]
  2. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  3. Bohl E. H., Theil K. W., Saif L. 1984; Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. Journal of Clinical Microbiology 19:105–111
    [Google Scholar]
  4. Both G. W., Bellamy A. R., Siegman L. J. 1982; A general strategy for cloning double-stranded RNA: nucleotide sequence of simian-11 rotavirus gene 8. Nucleic Acids Research 10:7045–7088
    [Google Scholar]
  5. Both G. W., Mattick J. S., Bellamy A. R. 1983; The serotype-specific glycoprotein of simian-11 rotaviruses: coding assignment and gene sequence. Proceedings of the National Academy of Sciences, U.S.A. 80:3091–3095
    [Google Scholar]
  6. Cashdollar L. W., Esparza J., Hudson G. R., Chmelo R., Lee P. W., Joklik W. K. 1982; Cloning the doublestranded RNA genes of reoviruses: sequence of the cloned S2 gene. Proceedings of the National Academy of Sciences, U.S.A. 79:7644–7648
    [Google Scholar]
  7. Chan W., Penaranda M. E., Crawford S. E., Estes M. K. 1986; Two glycoproteins are produced from the rotavirus neutralization gene. Virology 151:243–252
    [Google Scholar]
  8. Davis A., Kostek B., Mason B., Hsiao C. L., Morin J., Dheer S. K., Hung P. P. 1985; Expression of hepatitis B surface antigen with a recombinant adenovirus. Proceedings of the National Academy of Sciences, U.S.A. 82:7560–7564
    [Google Scholar]
  9. Dyall-Smith M., Holmes I. H. 1984; Sequence homology between human and animal rotavirus serotype-specific glycoproteins. Nucleic Acids Research 12:3973–3982
    [Google Scholar]
  10. Elleman T. C., Hoyne R. A., Dyall-Smith M. L., Holmes I. H., Azad A. A. 1983; Nucleotide sequence of the gene encoding the serotype-specific glycoprotein of U.K. bovine rotavirus. Nucleic Acids Research 11:4689–4701
    [Google Scholar]
  11. Ericson B., Graham D., Mason B., Estes M. K. 1982; Identification, synthesis, and modifications of simian rotavirus SA11 polypeptides in infected cells. Journal of Virology 42:825–839
    [Google Scholar]
  12. Ericson B., Graham D., Mason B., Hanssen H., Estes M. K. 1983; Two types of glycoprotein precursors are produced by the simian rotavirus SA11. Virology 127:320–332
    [Google Scholar]
  13. Estes M. K., Graham D., Ramig R. F., Ericson B. I. 1982; Heterogeneity in the structural glycoprotein (VP7) of simian rotavirus SA11. Virology 122:8–14
    [Google Scholar]
  14. Flewett T. H., Woode G. N. 1978; The rotaviruses. Archives of Virology 57:1–23
    [Google Scholar]
  15. Flores J., Greenberg H. B., Myslinski J. S., Kalica A. R., Wyatt R. G., Kapikian A. Z., Chanock R. M. 1982; Use of transcription probes for genotyping rotavirus reassortants. Virology 121:288–295
    [Google Scholar]
  16. Flores J., Boeggeman E., Purcell R. H., Sereno M., Perez L., White L., Wyatt R. G., Chanock R. M., Kapikian A. Z. 1983; A dot hybridization assay for detection of rotavirus. iMticet i:555–561
    [Google Scholar]
  17. Gaul S. K., Simpson T., Woode G. N., Fulton R. F. 1982; Antigenic relationships among some animal rotaviruses: virus neutralization in vitro and cross-protection in piglets. Journal of Clinical Microbiology 16:495–503
    [Google Scholar]
  18. Glass R., Keith J., Nakagomi O., Nakagomi T., Askaa J., Kapikian A. Z., Chanock R. M., Flores J. 1985; Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology 141:292–298
    [Google Scholar]
  19. Gorziglia M., Cashdollar L. W., Hudson G. R., Esparza J. 1983; Molecular cloning of a human rotavirus genome. Journal of General Virology 64:2585–2595
    [Google Scholar]
  20. Gorziglia M., Larrea C., Liprandi F., Esparza J. 1985; Biochemical evidence for the oligomeric (possibly trimeric) structure of the major inner capsid polypeptide (45K) of rotaviruses. Journal of General Virology 66:1889–1900
    [Google Scholar]
  21. Greenberg H., Flores J., Kalica A. R., Wyatt R. G., Jones R. 1983a; Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. Journal of General Virology 64:313–320
    [Google Scholar]
  22. Greenberg H., Valdesuso J., Van Wyke K., Midthun K., Walsh M., Mcauliffe V., Wyatt R. G., Kalica A. R., Flores J., Hoshino Y. 1983b; Production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus. Journal of Virology 47:267–275
    [Google Scholar]
  23. Gunn P., Sato F., Powell K., Bellamy R., Napier J., Harding D., Hancock W., Siegman L., Both G. 1985; Rotavirus neutralizing protein VP7: antigenic determinants investigated by sequence analysis and peptide synthesis. Journal of Virology 54:791–797
    [Google Scholar]
  24. Hoshino Y., Wyatt R. G., Scott F., Appell M. J. 1982; Isolation and characterization of a canine rotavirus. Archives of Virology 72:113–125
    [Google Scholar]
  25. Hoshino Y., Wyatt R. G., Greenberg H., Flores J., Kapikian A. Z. 1984; Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. Journal of Infectious Diseases 149:694–702
    [Google Scholar]
  26. Hoshino Y., Sereno M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. 1985; Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proceedings of the National Academy of Sciences, U.S.A. 82:8701–8704
    [Google Scholar]
  27. Hoshino Y., Sereno M. M., Midthun K., Flores J., Chanock R. M., Kapikian A. Z. 1986; Analysis of intertypic rotaviruses suggests that gene reassortment occurs in vivo. I. Studies by plaque reduction neutralization assay.. Journal of Clinical Microbiology (in press)
    [Google Scholar]
  28. Imai M., Richardson M. A., Ikegami N., Shatkin A. J., Furuichi Y. 1983; Molecular cloning of doublestranded RNA virus genomes. Proceedings of the National Academy of Sciences, U.S.A. 80:373–377
    [Google Scholar]
  29. Kapikian A. Z., Wyatt R. G., Greenberg H. B., Kalica A. R., Kim H., Brandt C. D., Rodriguez W. J., Parrot R. H., Chanock R. M. 1980; Approaches to immunization of infants and young children against gastroenteritis due to rotavirus. Reviews of Infectious Diseases 2:459–469
    [Google Scholar]
  30. Killen H. M., Dimmock N. J. 1982; Identification of a neutralization-specific antigen of a calf rotavirus. Journal of General Virology 62:297–311
    [Google Scholar]
  31. Kouvelos K., Petric M., Middleton P. J. 1984a; Oligosaccharide composition of calf rotavirus. Journal of General Virology 65:1159–1164
    [Google Scholar]
  32. Kouvelos K., Petric M., Middleton P. J. 1984b; Comparison of bovine, simian and human rotavirus structural glycoproteins. Journal of General Virology 65:1211–1214
    [Google Scholar]
  33. Kozak M. 1986; Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eucaryotic ribosomes. Cell 44:283–292
    [Google Scholar]
  34. Kyte J., Doolittle R. F. 1982; A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157:105–132
    [Google Scholar]
  35. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  36. Lazdins I., Sonza S., Dyall-Smith M. L., Coulson B. S., Holmes I. H. 1985; Demonstration of an immunodominant neutralization site by analysis of antigenic variants of SA11 rotavirus. Journal of Virology 56:317–319
    [Google Scholar]
  37. Mccrae M., Mccorquodale J. G. 1982a; The molecular biology of rotaviruses. II. Identification of the proteincoding assignments of calf rotavirus genome RNA species. Virology 117:435–443
    [Google Scholar]
  38. Mccrae M., Mccorquodale J. G. 1982b; Molecular biology of rotaviruses. IV. Molecular cloning of the bovine rotavirus genome. Journal of Virology 44:1076–1079
    [Google Scholar]
  39. Mason B., Graham D., Estes M. K. 1980; In vitro transcription and translation of simian rotavirus SA11 gene products. Journal of Virology 33:1111–1121
    [Google Scholar]
  40. Matsuno S., Inouye S. 1983; Purification of an outer capsid glycoprotein of neonatal calf diarrhea virus and preparation of its antisera. Infection and Immunity 39:155–158
    [Google Scholar]
  41. Maxam A. M., Gilbert W. 1977; A new method for sequencing DNA. Proceedings of the National Academy of Sciences, U. S. A 74:560–564
    [Google Scholar]
  42. Novo E., Esparza J. 1981; Composition and topography of structural polypeptides of bovine rotavirus. Journal of General Virology 56:325–335
    [Google Scholar]
  43. Perlman D., Halvorson H. O. 1983; A putative signal peptide recognition site and sequence in eukaryotic and prokaryotic signal peptides. Journal of Molecular Biology 167:391–409
    [Google Scholar]
  44. Richardson M. A., Iwamoto A., Ikegami N., Nomoto A., Furuichi Y. 1984; Nucleotide sequence of the gene encoding the serotype-specific antigen of human (Wa) rotaviruses: comparison with the homologous genes from simian SA11 and UK bovine rotaviruses. Journal of Virology 51:860–862
    [Google Scholar]
  45. Rixon F., Taylor P., Desselberger U. 1984; Rotavirus RNA segments sized by electron microscopy. Journal of General Virology 65:233–239
    [Google Scholar]
  46. Sabara M., Barrington A., Babiuk L. A. 1985a; Immunogenicity of a bovine rotavirus glycoprotein fragment. Journal of Virology 56:1037–1040
    [Google Scholar]
  47. Sabara M., Gilchrist J., Hudson G. R., Babiuk L. A. 1985b; Preliminary characterization of an epitope involved in neutralization and cell attachment that is located on the major bovine rotavirus glycoprotein. Journal of Virology 53:58–66
    [Google Scholar]
  48. Wyatt R. G., Greenberg H. B., James H. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. Z. 1982; Definition of human rotavirus serotypes by plaque reduction assay. Infection and Immunity 37:110–115
    [Google Scholar]
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