1887

Abstract

Summary

A panel of five monoclonal antibodies (MAbs) produced against the West Buxton isolate of infectious pancreatic necrosis virus was used to investigate viral antigens and to compare different aquatic birnavirus isolates antigenically. Reciprocal blocking ELISA and neutralization assays indicated that these MAbs identified four, and possibly five, structurally and/or functionally different epitopes on the virion. Western immunoblot analysis demonstrated that one epitope was present on VP2, the large (51000 mol. wt., 51K) capsid protein, and another epitope was located on the two smallest structural proteins, VP3 (32K) and VP4 (30K). Three MAbs did not react with any of the solubilized viral proteins; the epitopes recognized by these MAbs may have been altered when the virion was solubilized with SDS. Comparison of reactivity patterns of the five MAbs with various aquatic birnaviruses in ELISA and neutralization tests demonstrated that 14 isolates tested from four serotypes represented a minimum of nine antigenically distinct viruses; i.e., distinct patterns of reactivity were shown among several viruses within the same serotype. Two MAbs identified different epitopes that were highly conserved among, and largely restricted to, members of the West Buxton (U.S.A.) serotype, whereas two other MAbs recognized an epitope(s) present only on some members of this serotype. The other MAb defined an epitope that was more widely distributed among the aquatic birnaviruses and found on all representatives tested from two serotypes.

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1986-10-01
2022-01-28
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References

  1. Al Moudallal Z., Altschuh D., Briand J., Van Regenmortel M. H. V. 1984; Comparative sensitivity of different ELISA procedures for detecting monoclonal antibodies. Journal of Immunological Methods 68:35–43
    [Google Scholar]
  2. Dobos P., Roberts T. E. 1982; The molecular biology of infectious pancreatic necrosis virus: a review. Canadian Journal of Microbiology 29:377–384
    [Google Scholar]
  3. Dobos P., Hallett R., Kells D., Sorensen O., Rowe D. 1977; Biophysical studies of infectious pancreatic necrosis virus. Journal of Virology 22:150–159
    [Google Scholar]
  4. Dobos P., Hill B. J., Hallett R., Kells D. T. C., Bect H., Teninges D. 1979; Biophysical and biochemical characterization of five animal viruses with bisegmented double stranded RNA genomes. Journal of Virology 32:593–605
    [Google Scholar]
  5. Emini E., Kao S., Lewis A., Crainic R., Wimmer E. 1983; Functional basis of poliovirus neutralization determined with monospecific neutralizing antibodies. Journal of Virology 46:466–474
    [Google Scholar]
  6. Friguet B., Djavadi-Obaniance L., Paes J., Bussard A., Goldberg M. 1983; A convenient enzyme-linked immunosorbent assay for testing whether monoclonal antibodies recognize the same antigenic site: application to hybridomas specific for the β2-subunit of Escherichia coli tryptophan synthetase. Journal of Immunological Methods 60:351–358
    [Google Scholar]
  7. Hill B. J. 1976a; Molluscan viruses: their occurrence, culture, and relationships. In Proceedings of the 1st International Colloquium on Invertebrate Pathology pp 25–29 Kingston: Queens University Press;
    [Google Scholar]
  8. Hill B. J. 1976b; Comparative serology of IPN virus strains. Food and Agriculture Organization Aquaculture Bulletin 8:13
    [Google Scholar]
  9. Hill B. J., Way K. 1983; Serological classification of fish and shellfish bimaviruses. In Abstracts of First International Conference of the European Association of Fish Pathologists p 10 Plymouth
    [Google Scholar]
  10. Kärber G. 1931; Beitrag zur Kollektiven Behandlung Pharmakologischer Reihenversuche. Archiv für experimentelle Pathologie und Pharmakologie 162:480–483
    [Google Scholar]
  11. Kwan S. P., Yelton D. E., Sharff M. D. 1980; Production of monoclonal antibodies. In Genetic Engineering vol 2 pp 31–46 Edited by Setlow J. K., Hollaender A. New York: Plenum Press;
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Lannan C. N., Winton J. R., Fryer J. L. 1984; Fish cell lines: establishment and characterization of nine cell lines from salmonids. In Vitro 20:671–676
    [Google Scholar]
  14. Lubeck M. D., Gerhard W. 1981; Topological mapping of antigenic sites on the influenza A/PR/8/34 virus hemagglutinin using monoclonal antibodies. Virology 113:64–72
    [Google Scholar]
  15. Macdonald R. D., Gower D. A. 1981; Genomic and phenotypic divergence among three serotypes of aquatic birnavirus (infectious pancreatic necrosis virus). Virology 115:187–195
    [Google Scholar]
  16. Macdonald R. D., Yamamoto T. 1977; The structure of infectious pancreatic necrosis virus RNA. Journal of General Virology 34:235–247
    [Google Scholar]
  17. Nicholson B. L., Caswell P. 1982; Ezyme-linked immunosorbent assay for identification of infectious pancreatic necrosis virus. Journal of Clinical Microbiology 16:469–472
    [Google Scholar]
  18. Nicholson B. L., Pochebit S. 1981; Antigenic analysis of infectious pancreatic necrosis virus (IPNV) by neutralization kinetics. In International Symposium on Fish Biologies: Serodiagnostics and Vaccines pp 35–41 Edited by Anderson D. P., Hennersen W. Basel: S. Karger;
    [Google Scholar]
  19. Nicholson B. L., Thorne G. W., Janicki C., Hanson A. 1979; Studies on a host range variant from different isolates of infectious pancreatic necrosis virus (IPNV). Journal of Fish Diseases 2:367–379
    [Google Scholar]
  20. Okamoto N., Sano T., Hedrick R. P., Fryer J. L. 1983; Antigenic relationships of selected strains of IPNV and European eel virus. Journal of Fish Diseases 6:19–25
    [Google Scholar]
  21. Sano T. 1977; Viral diseases of cultured fishes in Japan. Fish Pathology 10:221–226
    [Google Scholar]
  22. Schulman M., Wilde G. D., Köhler G. 1978; A better cell line for making hybridomas secreting specific antibodies. Nature, London 276:269–270
    [Google Scholar]
  23. Sonstegaard R. A., McDermott L. A., Sonstegaard K. A. 1972; Isolation of infectious pancreatic necrosis virus from white suckers (Catastomus commersoni). Nature, London 236:174–175
    [Google Scholar]
  24. Stephens E. B., Nedsman M. W., Zachary A. L., Hetrick F. M. 1980; A viral aetiology for the annual spring epizootics in Atlantic menhaden, Brevoortia tyrannus, in the Chesapeake Bay. Journal of Fish Diseases 3:387–399
    [Google Scholar]
  25. Underwood B. O., Smale C. J., Brown F., Hill B. J. 1977; Relationship of a virus from Tellina tenuis to infectious pancreatic necrosis virus. Journal of General Virology 36:93–109
    [Google Scholar]
  26. Wolf K., Mann J. A. 1980; Poikilothermic vertebrate cell lines and viruses: a current listing of species. In Vitro 16:168–179
    [Google Scholar]
  27. Wolf K., Snieszko S. F., Dunbar C. E., Pyle E. 1960; Virus nature of infectious pancreatic necrosis in trout. Proceedings of the Society for Experimental Biology and Medicine 104:105–108
    [Google Scholar]
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