1887

Abstract

Summary

The production and testing of 10 rat monoclonal antibodies specific for strains of potato virus X (PVX) are described. Monoclonal antibodies (MAbs) with a variety of specificities were selected. At one extreme, MAb MAC 58 reacted with all 33 PVX isolates tested; in contrast, MAb MAC 67 reacted strongly with only two of 33 isolates, one of which was the resistance-breaking PVX-HB against which it was raised. The reactions of the PVX isolates with the MAbs were compared with the classification of PVX strains based on their effects in plants carrying different resistance genes; a preliminary serological classification is suggested. It is possible to distinguish PVX-HB from all other isolates tested using two MAbs (MAC 67 and MAC 59), and these, together with MAC 58, were produced in large quantities in ascitic fluids. Together they provide a valuable set of reagents for use in routine testing.

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/content/journal/jgv/10.1099/0022-1317-67-1-57
1986-01-01
2022-06-29
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References

  1. Bawden F. C., Sheffield F. M. L. 1944; The relationships of some viruses causing necrotic diseases of the potato. Annals of Applied Biology 31:33–40
    [Google Scholar]
  2. Beemster A. B. R., Rozendaal A. 1972; Potato viruses: properties and symptoms. In Viruses of Potatoes and Seed Potato Production pp 115–118 Edited by de Bokx A. J. Wageningen: Centre for Agricultural Publishing and Documentation;
    [Google Scholar]
  3. Briand J. P., Al Moudallal Z., Van Reoenmortel M. H. V. 1982; Serological differentiation of tobamoviruses by means of monoclonal antibodies. Journal of Virological Methods 5:293–300
    [Google Scholar]
  4. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  5. Cockerham G. 1955 Strains of potato virus X. Proceedings of the Second Conference on Potato Virus Diseases (Lisse-Wageningen, 1954) pp 89–92
    [Google Scholar]
  6. Cockerham G. 1970; Genetical studies on resistance to potato viruses X and Y. Heredity 25:309–348
    [Google Scholar]
  7. Fribourg C. E. 1975; Studies on potato virus X strains isolated from Peruvian potatoes. Potato Research 18:216–226
    [Google Scholar]
  8. Fribourg C. E. 1978; Antiserum production and present status of tests for viruses in the International Potato Center seed program. Proceedings, Planning Conference on Developments in the Control of Potato Virus Diseases (International Potato Center, Lima, Peru) pp 33–41
    [Google Scholar]
  9. Galfre G., Milstein C. 1981; Preparation of monoclonal antibodies: strategies and procedures. Methods in Enzymology 73B:3–46
    [Google Scholar]
  10. Galfre G., Milstein C., Wright B. 1979; Rat x rat hybrid myelomas and monoclonal anti-Fd portion of mouse IgG. Nature, London 211:131–133
    [Google Scholar]
  11. Jones R. A. C. 1982; Breakdown of potato virus X resistance gene Nx: selection of a group four strain from strain group three. Plant Pathology 31:325–331
    [Google Scholar]
  12. Jones R. A. C. 1985; Further studies on resistance-breaking strains of potato virus X. Plant Pathology 34:182–189
    [Google Scholar]
  13. Jones R. A. C., Fribourg C. E., Slack S. A. 1981; Potato virus and virus-like diseases. In Plant Virus Slide Series Set No. 2 Edited by Barnett O. W., Tollin S. A. South Carolina: Clemson University;
    [Google Scholar]
  14. Kilmartin J. V., Wright B., Milstein C. 1982; Rat monoclonal anti-tubulin antibodies derived by using a new non-secreting rat cell line. Journal of Cell Biology 93:576–582
    [Google Scholar]
  15. Matthews R. E. F. 1949; Studies on potato virus X. II.Criteria of relationships between strains. Annals of Applied Biology 36:460–474
    [Google Scholar]
  16. Moreira A., Jones R. A. C., Fribourg C. E. 1980; Properties of a resistance-breaking strain of potato virus X. Annals of Applied Biology 95:93–103
    [Google Scholar]
  17. Nakane P. K., Kawaoi A. 1974; Peroxidase-labelled antibody: a new method of conjugation. Journal of Histochemistry and Cytochemistry 22:1084–1091
    [Google Scholar]
  18. Van Regenmortel M. H. V. 1986; The potential for using monoclonal antibodies in the detection of plant viruses. In Developments and Applications in Virus Testing Edited by Jones C. R. A., Torrance L. Wellesboume: Association of Applied Biologists; (in press)
    [Google Scholar]
  19. Wright N. S. 1963; Detection of strain-specific serological activity in antisera to tobacco mosaic virus, clover yellow mosaic virus, and potato virus X, by complement fixation. Virology 20131–136
    [Google Scholar]
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