Cowpea protoplasts became infected following inoculation either with particles of cymbidium ringspot virus (CyRSV) or with CyRSV RNA. About 50% of the protoplasts were infected by inocula that contained polyethylene glycol, but fewer were infected by inocula that contained poly--ornithine. About 20 pg of virus accumulated per infected protoplast. Cordycepin and actinomycin D inhibited virus multiplication in protoplasts inoculated with virus particles or with RNA, lowering both the proportion of protoplasts becoming infected and the virus yield per infected protoplast. Infected protoplasts supported the replication of a satellite RNA and reacted cytologically to infection by forming multivesicular bodies presumably derived from peroxisomes.


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