1887

Abstract

SUMMARY

Fourteen mouse monoclonal antibodies raised against tick-borne encephalitis virus (TBEV) and polyclonal antisera raised against six other flaviviruses, Edge Hill (EHV), Japanese encephalitis (JEV), Langat (LGTV), louping ill (LIV), West Nile (WNV) and yellow fever (YFV), were tested for their ability to enhance the replication of TBEV in cells of the mouse macrophage-like line P388 D1, and for their reactivity in ELISA and haemagglutination inhibition (HI) tests. Irrespective of their specificity for either the 51K or 58K polypeptide present in TBEV-infected cells, 13 of the 14 monoclonal antibodies enhanced the replication of TBEV but not of WNV. The remaining monoclonal antibody, which immunoprecipitated the 58K polypeptide of TBEV enhanced WNV but not TBEV, although it reacted strongly with both viruses in ELISA and HI tests. Only polyclonal antisera against viruses within the tick-borne encephalitis virus complex (TBEV, LGTV and LIV) enhanced TBEV replication, although all the polyclonal antisera reacted with TBEV by ELISA; two (against JEV and WNV) also reacted by HI test and all enhanced the replication of WNV. These findings suggest that with TBEV, enhancement may be TBEV complex-specific rather than flavivirus-specific. Data derived from testing both polyclonal and monoclonal antibodies suggest further that not all antibodies that bind to the envelope glycoprotein of TBEV are able to enhance the replication of TBEV, and that enhancement is epitope-specific.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-8-1831
1985-08-01
2021-10-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/8/JV0660081831.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-8-1831&mimeType=html&fmt=ahah

References

  1. Bannatyne C. H., Wilson R. L., Reid H. W., Burton O., Pow I. 1980; Louping 111 virus infection of pigs. Veterinary Record 106:13
    [Google Scholar]
  2. Brandt W. E., Mccown J. M., Gentry M. K., Russell P. K. 1982; Infection enhancement of dengue type 2 virus in the U-937 human monocyte cell line by antibodies to flavivirus cross-reactive determinants. Infection and Immunity 36:1036–1041
    [Google Scholar]
  3. Burstin S. J., Brandriss M. W., Schlesinger J. J. 1983; Infection of a macrophage cell line, P388 D1 with reovirus: effects of immune ascitic fluids and monoclonal antibodies on neutralisation and on enhancement of viral growth. Journal of Immunology 130:2915–2919
    [Google Scholar]
  4. Cafruny W. A., Plagemann P. G. W. 1982; Immune response to lactate dehydrogenase-elevating virus: serologically specific rabbit neutralising antibody to the virus. Infection and Immunity 37:1007–1012
    [Google Scholar]
  5. Clarke D. H., Casals J. 1958; Techniques for hemagglutination and hemagglutination inhibition with arthropod borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  6. Clerx J. P. M., Horzinek M. C., Osterhaus A. D. M. E. 1978; Neutralization and enhancement of infectivity of non-salmonid fish rhabdoviruses by rabbit and pike immune sera. Journal of General Virology 40:297–308
    [Google Scholar]
  7. Daughaday C. C., Brandt W. E., Mccown J. M., Russell P. K. 1981; Evidence for two mechanisms of dengue virus infection of adherent human monocytes: trypsin-sensitive virus receptors and trypsin-resistant immune complex receptors. Infection and Immunity 32:469–473
    [Google Scholar]
  8. Detre K., White C. 1970; The comparison of two Poisson-distributed observations. Biometrics 26:851–854
    [Google Scholar]
  9. Halstead S. B. 1982; Immune enhancement of viral infection. Progress in Allergy 31:301–364
    [Google Scholar]
  10. Halstead S. B., Venkateshan C. N., Gentry M. K., Larsen L. K. 1984; Heterogeneity of infection enhancement of dengue 2 strains by monoclonal antibodies. Journal of Immunology 132:1529–1532
    [Google Scholar]
  11. Hambleton P., Stephenson J. R., Baskerville A., Wiblin C. N. 1983; Tick-borne encephalitis in primates, pathology, immune response and efficacy of vaccination. Infection and Immunity 40:995–1003
    [Google Scholar]
  12. Heinz F. X., Tuma W., Kunz C. H. 1981; Antigenic and immunogenic properties of defined physical forms of tick-borne encephalitis virus structural proteins. Infection and Immunity 33:250–257
    [Google Scholar]
  13. King A. A., Sands J. J., Porterfield J. S. 1984; Antibody-mediated enhancement of rabies virus infection in a mouse macrophage cell line (P388 Dl). Journal of General Virology 65:1091–1093
    [Google Scholar]
  14. Koren J. H., Handwerger B. S., Wunderlich J. R. 1975; Identification of macrophage-like characteristics in a cultured murine tumour line. Journal of Immunology 114:894–897
    [Google Scholar]
  15. Lowry O. H., Rosebrough N. J., Farr A. L., Randall R. J. 1951; Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry 193:265–275
    [Google Scholar]
  16. Madrid DE A. T., Porterfield J. S. 1969; A simple micro-cellular method for the study of group B arboviruses. Bulletin of the World Health Organization 40:113–121
    [Google Scholar]
  17. Madrid DE A. T., Porterfield J. S. 1974; The flaviviruses (group B arboviruses): a cross-neutralization study. Journal of General Virology 23:91–96
    [Google Scholar]
  18. Peiris J. S. M., Porterfield J. S. 1981; Antibody-dependent enhancement of plaque formation on cell lines of macrophage origin – a sensitive assay for antiviral antibody. Journal of General Virology SI119–125
    [Google Scholar]
  19. Peiris J. S. M., Porterfield J. S. 1982; Antibody-dependent plaque enhancement: its antigenic specificity in relation to Togaviridae. Journal of General Virology 58:291–296
    [Google Scholar]
  20. Peiris J. S. M., Gordon S., Unkeless J. C., Porterfield J. S. 1981; Monoclonal anti-Fc receptor IgG blocks antibody enhancement of viral replication in macrophages. Nature, London 289:189–191
    [Google Scholar]
  21. Porterfield J. S. 1982; Immunological enhancement and the pathogenesis of dengue haemorrhagic fever. Journal of Hygiene 89:355–364
    [Google Scholar]
  22. Prabhakar B. S., Nathanson N. 1981; Acute rabies death mediated by antibody. Nature, London 290:590–591
    [Google Scholar]
  23. Reid H. W., Duncan J. S., Phillips J. D. P., Moss R., Watson A. 1978; Studies on Louping 111 virus (flavivirus group) in wild red grouse (Lagopus logapus scoticus). Journal of Hygiene 81:321–330
    [Google Scholar]
  24. Stephenson J. R., Lee J. M., Wilton-smith P. D. 1984; Antigenic variation among members of the tick-borne encephalitis complex. Journal of General Virology 65:81–89
    [Google Scholar]
  25. Weiss R. W., Scott F. W. 1981; Antibody-mediated enhancement of disease in feline infectious peritonitis: comparisons with dengue haemorrhagic fever. Comparative Immunology, Microbiology and Infectious Diseases 4:175–189
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-8-1831
Loading
/content/journal/jgv/10.1099/0022-1317-66-8-1831
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error