dIII fragments D to P of DNA from a Chinese vaccine strain (Tian Tan) of vaccinia virus have been molecularly cloned into the plasmid pAT153 at the unique dIII site. The Chinese strain DNA differs from a non-vaccine American strain (WR) in having an additional dIII fragment (P). Twelve dIII D clones and 12 dIII F clones of the Chinese strain were analysed by digestion by RI, HI, I and I. Two forms of D (designated a and b) and of F (a and b) were demonstrated. In each the differences were detected as the presence of an additional RI site, in Da and Fa. The dIII Fa and Fb fragments of the Chinese strain were shown to differ significantly from the WR strain in their restriction site maps.


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