1887

Abstract

SUMMARY

Two conditional transcriptase-negative mutants of vesicular stomatitis virus (VSV) serotype New Jersey, B1 and F1, their revertants B1/R1 and F1/R1 and the wild-type virus were dissociated into pellet, NS and L fractions and, after reconstitution of these in various combinations, the transcriptase activities were assayed at the permissive (31 °C) and restrictive (39 °C) temperatures. The pellet fractions contained the virion RNA-polypeptide N complexes, while the NS and L fractions were essentially pure preparations of these polypeptides. The synthesis of RNA by the reconstituted pellet and L fractions was inhibited at 39 °C only when the L fractions of B1 or F1 were used. Addition of the NS fractions to the reconstituted pellet and L fractions did not alter the rates of RNA synthesis. These results demonstrate that polypeptide L is the temperature-sensitive polypeptide of both mutants B1 and F1 and support previous observations that polypeptide L is the transcriptase itself. The fact that a second mutant of complementation group F, F2, is transcriptase-positive but replicase-negative suggests that polypeptide L is involved both in transcription and replication. Intracistronic complementations may account for the observation that the temperature-sensitive mutations affect polypeptide L in complementation groups B and F.

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/content/journal/jgv/10.1099/0022-1317-66-7-1507
1985-07-01
2021-10-26
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