1887

Abstract

SUMMARY

The Australian isolate of infectious bursal disease (IBD) virus (002/73) was purified from infected bursae by rate-zonal and density-equilibrium centrifugation and characterized by polyacrylamide gel electrophoresis. Two major polypeptides having approximate mol. wt. of 32000 (32K) and 37K and three other polypeptides of approximate mol. wt. 29K, 41.5K and 91.5K were present in all preparations of virus having a buoyant density of 1.33 g/ml. Western blotting of the polypeptides of IBD virus showed that the initial antibody response of chickens infected with live virus or injected with an inactivated oil-emulsion vaccine was directed primarily towards the 32K polypeptide. Only sera obtained late in the response to live virus or following hyperimmunization contained antibodies recognizing the 29K, 37K and 41.5K polypeptides. An antibody response to the 91.5K polypeptide was not detected routinely by this technique. It was concluded that the 32K polypeptide is a major immunogen of IBD virus.

Keyword(s): IBD virus , immunogens and polypeptides
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/content/journal/jgv/10.1099/0022-1317-66-7-1479
1985-07-01
2024-10-06
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References

  1. Azad A. A., Barrett S. A., Fahey K. J. 1985; The characterization and molecular cloning of the double-stranded RNA genome of an Australian strain of infectious bursal disease virus. Virology (in press)
    [Google Scholar]
  2. Burnette W. N. 1981; ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  3. Cohen B. B., Moxley M., Crichton D., Deane D. L., Steel C. M. 1984; A mild procedure for separating polypeptide chains prior to immunoprecipitation and Western blotting analysis. Journal of Immunological Methods 75:99–105
    [Google Scholar]
  4. Dobos P. 1979; Peptide map comparison of the proteins of infectious bursal disease virus. Journal of Virology 32:1046–1050
    [Google Scholar]
  5. Firth G. A. 1974; Occurrence of an infectious bursal syndrome within an Australian poultry flock. Australian Veterinary Journal 50:128–130
    [Google Scholar]
  6. Higgins D. A. 1975; Physical and chemical properties of fowl immunoglobulins. Veterinary Bulletin 45:139–154
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. McMaster G. K., Carmichael G. C. 1977; Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proceedings of the National Academy of Sciences, U.S.A 74:4835–4838
    [Google Scholar]
  9. Müller H., Becht H. 1982; Biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their significance as structural elements for infectious virus and incomplete particles. Journal of Virology 44:384–392
    [Google Scholar]
  10. Müller H., Scholtissek C., Becht H. 1979; The genome of infectious bursal disease virus consists of two segments of double-stranded RNA. Journal of Virology 31:584–589
    [Google Scholar]
  11. Nick H., Cursiefen G., Becht H. 1976; Structural and growth characteristics of infectious bursal disease virus. Journal of Virology 18:227–234
    [Google Scholar]
  12. Todd D., McNulty M. S. 1979; Biochemical studies with infectious bursal disease virus: comparison of some of its properties with infectious pancreatic necrosis virus. Archives of Virology 60:265–277
    [Google Scholar]
  13. Wyeth P. J., Chettle N. 1982; Comparison of the efficacy of four inactivated infectious bursal disease oil emulsion vaccines. Veterinary Record 110:359–361
    [Google Scholar]
  14. Wyeth P. J., O’Brien J. D. P., Cullen G. A. 1981; Improved performance of progeny of broiler parent chickens vaccinated with infectious bursal disease oil-emulsion vaccine. Avian Diseases 25:228–241
    [Google Scholar]
  15. York J. J., Fahey K. J., Bagust T. J. 1983; Development and evaluation of an ELISA for the detection of antibody to infectious laryngotracheitis virus in chickens. Avian Diseases 27:409–421
    [Google Scholar]
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