1887

Abstract

SUMMARY

Simian foamy virus type 1 (SFV-1), the prototype of the Spumavirinae, was subjected to disruption and serial purification procedures. Separation of SFV-1 envelope components from viral cores was verified by electron microscopy, density gradient centrifugation and polyacrylamide gel electrophoresis. After affinity chromatography of the envelope polypeptides on a concanavalin A-Sepharose column, a highly purified 70000 mol. wt. protein was recovered. Glycosylation of this gp70 was confirmed by glucosamine labelling. Immunological studies with anti-SFV antisera confirmed the type-specificity of this envelope gp70.

Keyword(s): envelope , glycoprotein and SFV-1
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/content/journal/jgv/10.1099/0022-1317-66-7-1449
1985-07-01
2024-12-06
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References

  1. Achong B. G., Mansell P. W. A., Epstein M. A., Clifford P. 1971; An unusual virus in cultures from a human nasopharyngeal carcinoma. Journal of the National Cancer Institute 46:299–307
    [Google Scholar]
  2. Benzair A. B., Rhodes-Feuillette A., Emanoil-Ravicovitch R., Périés J. 1982; Reverse transcriptase from simian foamy virus serotype 1: purification and characterization. Journal of Virology 44:720–724
    [Google Scholar]
  3. Benzair A. B., Rhodes-Feuillette A., Emanoil-Ravicovitch R., Périés J. 1983; Characterization of RNase H activity associated with reverse transcriptase in simian foamy virus type 1. Journal of Virology 47:249–252
    [Google Scholar]
  4. Brenner S., Horne R. W. 1959; A negative staining method for high resolution electron microscopy of viruses. Biochimica et biophysica acta 34:103–110
    [Google Scholar]
  5. Brown P., Nemo G., Gajdusek D. C. 1978; Human foamy virus: further characterization, seroepidemiology and relationship to chimpanzee foamy viruses. Journal of Infectious Diseases 4:421–427
    [Google Scholar]
  6. Cavalieri F., Rhodes-Feuillette A., Benzair A. B., Emanoil-Ravicovitch R., Périés J. 1981; Biochemical characterization of simian foamy virus type 1. Archives of Virology 68:197–202
    [Google Scholar]
  7. Epstein M. A., Achong B. G., Ball G. 1974; Further observations on a human syncytial virus from a nasopharyngeal carcinoma. Journal of the National Cancer Institute 53:681–688
    [Google Scholar]
  8. Hooks I. J., Gibbs C. J. 1975; The foamy viruses. Bacteriological Reviews 39:169–185
    [Google Scholar]
  9. Hooks J. J., Hooks B. D. 1981; Spumavirinae: foamy virus group infections: comparative aspects and diagnosis. In Comparative Diagnosis of Viral Diseases vol 4: pp 599–618 Edited by Kurstak K., Kurstak C. New York: Academic Press;
    [Google Scholar]
  10. Johnston P. B. 1971; Taxonomic features of seven serotypes of simian and ape foamy viruses. Infection and Immunity 3:793–799
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Ouchterlony O. 1962; Diffusion in gel methods of immunological analysis. Progress in Allergy 6:30–35
    [Google Scholar]
  13. Périés J., Todaro G. J. 1977; A haemagglutination assay for the primate syncytium-forming (foamy) viruses. Journal of General Virology 34:195–199
    [Google Scholar]
  14. Rhodes-Feuillette A., Saal F., Lasneret J., Dubouch P., Périés J. 1979; Isolation and characterization of a new simian foamy virus serotype from lymphocytes of a Papiocynocephalus baboon. Journal of Medical Primatology 8:308–320
    [Google Scholar]
  15. Stephenson J. R. (editor) 1980 Molecular Biology of RNA Tumor Viruses New York: Academic Press;
    [Google Scholar]
  16. Strand M., August J. T. 1976; Structural proteins of ribonucleic acid tumor viruses. Journal of Biological Chemistry 251:559–564
    [Google Scholar]
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