1887

Abstract

SUMMARY

When RNA from particles of Indian peanut clump virus (IPCV) was translated using messenger-dependent reticulocyte lysate the largest product had a mol. wt. of about 195000 and the other main products had mol. wt. of 143000 and 24500. The 24500 mol. wt. polypeptide co-migrated with IPCV coat protein and was the only product that reacted with antiserum to IPCV particles. Translation of separated RNA species showed that the 195000 mol. wt. and 143000 mol. wt., as well as some smaller less abundant products, arose from translation of RNA-1 (mol. wt. 1.9 × 10) whereas RNA-2 (mol. wt. 1.6 × 10) was translated to give only the 24500 mol. wt. product Translation of RNA of mol. wt. 0.5 × 10 to 0.9 × 10 gave a 50000 mol. wt. product that was present and sometimes prominent in translation products of unfractionated RNA. A product of about 20000 mol. wt. was made by translation of RNA of about 0.2 × 10 mol. wt. IPCV resembles the viruses with bipartite genomes whose larger RNA species is translated into large polypeptides and whose coat protein gene is on the smaller RNA species. The other translation products may be those of subgenomic messenger, or of satellite, RNA species.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-66-6-1347
1985-06-01
2021-10-25
Loading full text...

Full text loading...

/deliver/fulltext/jgv/66/6/JV0660061347.html?itemId=/content/journal/jgv/10.1099/0022-1317-66-6-1347&mimeType=html&fmt=ahah

References

  1. Brandt T. L., Hackett P. B. 1983; Characterization of messenger RNA by direct translation from agarose gels. Analytical Biochemistry 135:401–408
    [Google Scholar]
  2. Estes A. P., Brakke M. K. 1966; Correlation of Polymyxa graminis with transmission of soil-borne wheat mosaic virus. Virology 28:772–774
    [Google Scholar]
  3. Hirth L., Richards K. E. 1981; Tobacco mosaic virus: model for structure and function of a simple virus. Advances in Virus Research 26:145–199
    [Google Scholar]
  4. Hsu Y. H., Brakke M. K. 1983; Translation of soil-borne wheat mosaic virus RNAs in rabbit reticulocyte lysate (abstract).. Phytopathology 73:790
    [Google Scholar]
  5. Hsu Y. H., Brakke M. K. 1985; Sequence relationships among soil-borne wheat mosaic virus RNA species and terminal structures of RNA II. Journal of General Virology 66:915–919
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  7. Murant A. F., Mayo M. A. 1982; Satellites of plant viruses. Annual Review of Phytopathology 20:49–70
    [Google Scholar]
  8. Putz C., Pinck M., Fritsch C., Pinck L. 1983; Identification of the 3′ -and 5′-ends of beet necrotic yellow vein virus RNAs. FEBS Letters 156:41–46
    [Google Scholar]
  9. Reddy D. R., Rajeshwari R., NZUKA N., Lesemann D. E., Nolt B. L., Goto T. 1983; The occurrence of Indian peanut clump, a soil-borne virus disease of groundnuts (Arachis hypogaea) in India. Annals of Applied Biology 102:305–310
    [Google Scholar]
  10. Richards K., Jonard G., Guilley H., Ziegler V., Putz C. 1985; In vitro translation of beet necrotic yellow vein virus RNA and studies of sequence homology among the RNA species using cloned cDNA probes. Journal of General Virology 66:345–350
    [Google Scholar]
  11. Brakke Shirako Y. 1984; Two purified RNAs of soil-borne wheat mosaic virus are needed for infection. Journal of General Virology 65:119–127
    [Google Scholar]
  12. Thouvenel J., Fauquet C. 1981a; Further properties of peanut clump virus and studies on its natural transmission. Annals of Applied Biology 97:99–107
    [Google Scholar]
  13. Thouvenel J. C., Fauquet C. 1981b; Peanut clump virus. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses no. 235
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-66-6-1347
Loading
/content/journal/jgv/10.1099/0022-1317-66-6-1347
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error