Infective clones of the Nigerian isolate of cassava latent virus (CLV) have been obtained. The apparent molecular weight of the capsid protein of this isolate is slightly higher than that produced in plants infected with cloned DNAs of the Kenyan isolate of CLV. Pseudorecombinant experiments using heterologous combinations of cloned DNAs have confirmed that the physical properties of the capsid protein are encoded on DNA 1 and at least some determinants of symptom induction are also located on this DNA. Comparison between the nucleotide sequences of the open reading frames encoding the two capsid proteins shows several nucleotide differences which affect the amino acid composition but which do not significantly alter the potential molecular weight of the product. translation of poly(A) RNAs shows that the differences in electrophoretic mobility are not due to differences in host-directed post-translational processing.


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