1887

Abstract

SUMMARY

The lipid compositions of membrane vesicles from scrapie-infected and uninfected hamster brains were examined before and after detergent extraction. No differences were observed in polar lipids, glycolipids, gangliosides or neutral lipids examined by thin-layer chromatography. Analysis of detergent-extracted CsCl gradient fractions with high scrapie infectivity failed to reveal any glycerolphosphatides, although neutral lipids were demonstrated. The major neutral lipid associated with detergent-extracted membrane vesicles from both infected and uninfected brain was an unidentified lipid which was found to absorb u.v. radiation strongly from 250 to 300 nm wavelengths. Membrane neutral lipids that strongly absorb u.v. radiation at wavelengths normally used to inactivate viruses may protect a small nucleic acid essential for scrapie infectivity.

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1985-04-01
2024-05-13
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References

  1. Alper T., Cramp W. A., Haig D. A., Clarke M. C. 1967; Does the scrapie agent replicate without a nucleic acid?. Nature, London 214:764–765
    [Google Scholar]
  2. Annunziata P., Federico A. 1981; Brain glycosidases in Creutzfeldt-Jakob disease. Journal of the Neurological Sciences 49:325–328
    [Google Scholar]
  3. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 55:297–302
    [Google Scholar]
  4. Cho H. J. 1983; Inactivation of the scrapie agent by Pronase. Canadian Journal of Comparative Medicine 47:494–496
    [Google Scholar]
  5. Cooperstock M., Riegle L. 1981; Polymyxin B inactivation of lipopolysaccharide in vaccines of Gramnegative bacteria. Injection and Immunity 33:315–318
    [Google Scholar]
  6. Dees C., German T. L., Wade W. F., Marsh R. F. 1985; Characterization of proteins in membrane vesicles from scrapie-infected hamster brain. Journal of General Virology 66:851–859
    [Google Scholar]
  7. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of 131 I-labelled human growth hormone of high specific radioactivity. Biochemical Journal 89:114–123
    [Google Scholar]
  8. Jorgenson P. L. 1982; Mechanism of the Na+, K+ pump. Protein structure and conformation of the pure (Na+, K+)-ATPase. Biochimica et biophysica acta 694:27–68
    [Google Scholar]
  9. Kates M. 1975; Techniques of lipidology. In Laboratory Techniques in Biochemistry and Molecular Biology pp 435–444 Edited by Work T. S., Work E. Amsterdam: North-Holland;
    [Google Scholar]
  10. Kimberlin R. H. 1982; Reflections on the nature of the scrapie agent. Trends in Biochemical Science 1:393–394
    [Google Scholar]
  11. Kimberlin R. H., Marsh R. F. 1975; Comparison of scrapie and transmissible mink encephalopathy in hamsters. I. Biochemical studies of the brain during development of disease. Journal of Infectious Diseases 131:97–103
    [Google Scholar]
  12. Latarjet R. 1979; Inactivation of the agents of scrapie, Creutzfeldt-Jakob disease and kuru by radiations. In Slow Transmissible Diseases of the Nervous System vol 2: pp 397–407 Edited by Prusiner S. B., Hadlow W. J. New York: Academic Press;
    [Google Scholar]
  13. Lewin P. K. 1982; Infectious peptide: postulated mechanisms of protovirion replication in scrapie. Canadian Medical Association Journal 127:471–472
    [Google Scholar]
  14. Marsh R. F., Dees C., Castle B. E., Wade W. F., German T. L. 1984; Purification of the scrapie agent by density gradient centrifugation. Journal oj General Virology 65:415–421
    [Google Scholar]
  15. Millson G. C., Hunter G. D., Kimberlin R. H. 1971; An experimental examination of the scrapie agent in cell mixtures. II. Association of scrapie activity with membrane fractions. Journal of Comparative Pathology 81:255–265
    [Google Scholar]
  16. Millson G. C., Hunter G. D., Kimberlin R. H. 1976; The physico-chemical nature of the scrapie agent. In Slow Virus Diseases of Animals and Man pp 243–266 Edited by Kimberlin R. H. Amsterdam & Oxford: North-Holland;
    [Google Scholar]
  17. Nelson G. J. 1975; Isolation and purification of lipids from animal tissues. In Analysis of Lipids and Lipoproteins pp 1–22 Edited by Perkins E. G. Champaign: American Oil Chemists Society;
    [Google Scholar]
  18. Prusiner S. B. 1982; Novel proteinaceous infectious particles cause scrapie. Science 216:136–144
    [Google Scholar]
  19. Prusiner S. B., Cochran S. P., Groth D. F., Bowman K. A., Martinez H. M. 1981a; Measurement of the scrapie agent using an incubation time interval assay. Annals of Neurology 11:353–358
    [Google Scholar]
  20. Prusiner S. B., Mckinley M. P., Groth D. F., Bowman K. A., Mock N. I., Cochran S. P., Masiarz F. R. 1981b; Scrapie agent contains a hydrophobic protein. Proceedings of the National Academy of Sciences, U,. S,. A 78:6675–6679
    [Google Scholar]
  21. Rutter G., Asher D. M., Rohwer R. G., Gibbs C. J., Gajdusek D. C. 1981; Increased concanavalin A capping in cells from brains of scrapie-infected hamsters. Archives of Virology 68:129–133
    [Google Scholar]
  22. Semancik J. S., Marsh R. F., Geelen J. L., Hanson R. P. 1976; Properties of the scrapie agent-endomembrane complex from hamster brain. Journal of Virology 18:693–700
    [Google Scholar]
  23. Shands J. H., Noble R. C. 1980; Quantitation of lipid mass by liquid scintillation counting procedure following charring on thin-layer plates. Analytical Biochemistry 101:427–434
    [Google Scholar]
  24. Viret J., Dormont D., Molle D., Court L., Leterrier F., Cathala F., Gibbs C. J., Gajdusek D. C. 1981; Structural modifications of nerve membranes during experimental scrapie evolution in mouse. Biochemical and Biophysical Research Communications 101:830–836
    [Google Scholar]
  25. Vostroknutova G. N., Bulgakova V. G., Udalva T. P., Sepetov N. F., Sibel’dina L. A. 1981; Localization of gramicidins on the cytoplasmic membrane of Bacillus brevis and its effect on the activity of membrane enzymes. Biokhimiya 46:657–666
    [Google Scholar]
  26. Yu R. U., Manuelidis E. E. 1978; Ganglioside alterations in guinea pig brains at end stages of experimental Creutzfeldt-Jakob disease. Journal of the Neurological Sciences 6:15–23
    [Google Scholar]
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