1887

Abstract

SUMMARY

Density gradient fractions prepared from healthy or scrapie-infected hamster brain tissue enriched in plasma membrane vesicles were treated with nucleases prior to phenol extraction and ethanol precipitation. The recovered nucleic acids were 3′ end-labelled and run on one-dimensional polyacrylamide gels. Autoradiography revealed the presence of low molecular weight RNAs (4S) in both healthy and scrapie samples. Two-dimensional fingerprint analysis indicated that the RNAs isolated from scrapie-infected hamsters contained oligonucleotides that were not present in RNAs isolated from healthy hamsters.

Keyword(s): oligonucleotides , RNA and scrapie
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/content/journal/jgv/10.1099/0022-1317-66-4-839
1985-04-01
2024-10-13
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