1887

Abstract

SUMMARY

Protein kinase has been extracted in soluble form from virions of pseudorabies virus using 10% NP40, 0.6 -NaCl. Chromatographic analysis of the extract on DEAE-cellulose and on phosphocellulose showed it to contain more than one kinase. The activity responsible for the phosphorylation of the major phosphoproteins (mol. wts. 120000, 115000 and 72000) of virions was found to be similar in its properties to the host enzyme casein kinase II. Purified casein kinase II from ascites cells or from pig liver was able to phosphorylate heat-inactivated virions. In addition to the major phosphoproteins, active virion preparations were able to phosphorylate a minor low molecular weight phosphoprotein, incorporation into which could be stimulated by the addition of cyclic AMP to the assay. Purified host cyclic AMP-dependent protein kinase also phosphorylated this protein in heat-inactivated virions. Analysis of herpes simplex virus type 1 showed that the major phosphoproteins (VP12 and VP23) could be phosphorylated in heat-inactivated virions by added casein kinase II. One of these (VP12) together with a further minor phosphoprotein (VP14) could be phosphorylated by cyclic AMP-dependent protein kinase.

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/content/journal/jgv/10.1099/0022-1317-66-4-661
1985-04-01
2024-04-26
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References

  1. Chantler J. K., Stevely W. S. 1973; Virus-induced proteins in pseudorabies-infected cells. I. Acid-extractable proteins of the nucleus. Journal of Virology 11:815–822
    [Google Scholar]
  2. Flugel R. M., Darai G. 1982; Protein kinase and specific phosphate acceptor proteins associated with tupaia herpes virus. Journal of Virology 43:410–415
    [Google Scholar]
  3. Hathaway G. M., Traugh J. A. 1982; Casein kinases – multipotential protein kinases. Current Topics in Cellular Regulation 21:101–127
    [Google Scholar]
  4. Hathaway G. M., Lubben T. H., Traugh J. A. 1980; Inhibition of casein kinase II by heparin. Journal of Biological Chemistry 255:8038–8041
    [Google Scholar]
  5. Kaplan A. S., Vatter A. E. 1959; A comparison of herpes simplex and pseudorabies viruses. Virology 7:394–407
    [Google Scholar]
  6. Lemaster S., Roizman B. 1980; Herpes simplex virus phosphoproteins. II. Characterisation of the virion protein kinase and of the polypeptides phosphorylated in the virion. Journal of Virology 35:798–811
    [Google Scholar]
  7. Lodish H. F., Porter M. 1980; Specific incorporation of host cell surface proteins into budding vesicular stomatitis virus particles. Cell 19:161–169
    [Google Scholar]
  8. McGarvey M. J., Leader D. P. 1983; The ribosomal proteins phosphorylated in vitro by protein kinase activities from Krebs II ascites cells. Bioscience Reports 3:621–629
    [Google Scholar]
  9. McGrath B. M., Stevely W. S. 1980; The characteristics of the cell-free translation of mRNA from cells infected with the herpes virus pseudorabies virus. Journal of General Virology 49:323–332
    [Google Scholar]
  10. Randall C. C., ROGERS H. W., Downer D. N., Gentry G. A. 1972; Protein kinase activity in equine herpes virus. Journal of Virology 9:216–222
    [Google Scholar]
  11. Rosok M. J., Watson K. F. 1979; Fractionation of two protein kinases from avian myeloblastosis virus and characterisation of the protein kinase activity preferring basic phosphoacceptor proteins. Journal of Virology 29:872–880
    [Google Scholar]
  12. Rubenstein A. S., Gravell M., Darlington R. 1972; Protein kinase in enveloped herpes simplex virions. Virology 50:287–290
    [Google Scholar]
  13. Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. V. Purification and structural proteins of the herpes virion. Journal of Virology 9:143–159
    [Google Scholar]
  14. Stevely W. S. 1975; Virus-induced proteins in pseudorabies-infected cells. II. Proteins of the virion and nucleocapsid. Journal of Virology 16:944–950
    [Google Scholar]
  15. Takai Y., Kishimoto A., Iwasa Y., Kawahara Y., Mori T., Nishizuka Y. 1979; Calcium-dependent activation of a multifunctional protein kinase by membrane phospholipids. Journal of Biological Chemistry 254:3692–3695
    [Google Scholar]
  16. Tan K. B. 1975; Comparative study of the protein kinase associated with animal viruses. Virology 64:566–570
    [Google Scholar]
  17. Tyler S. J., Hay J., Stevely W. S. 1974; Nuclear protein synthesis in hamster kidney cells: the effect of pseudorabies virus infection. Archiv für die gesamte Virusforschung 44:84–91
    [Google Scholar]
  18. Weis J. H., Faras A. J. 1983; Purification and characterisation of two protein kinases associated with Rous sarcoma virus. Biochemistry 22:165–170
    [Google Scholar]
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